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Jin Wang, Yue Liu, Xueliang Chen, and Qiusheng Kong

Transcription factors (TFs) are extensively involved in the regulation of plant growth and development. MYB is an important TF gene family with the largest members ( Dubos et al., 2010 ). Its N-terminal region has a highly conserved DNA binding

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Hongmei Ma, Margaret Pooler, and Robert Griesbach

to DNA. The Z. mays anthocyanin regulatory genes Lc and C1 were the first plant MYC and MYB genes, respectively, to be isolated. Recently, WD40 repeat (WDR) proteins were also recognized as part of the anthocyanin regulatory network ( deVetten

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Hongmei Ma, Margaret Pooler, and Robert Griesbach

involved in anthocyanin regulation ( Grotewold et al., 2000 ; Lesnick and Chandler, 1998 ). Both the Myc and Myb gene families contain members that have arisen by gene duplication ( Hanson et al., 1996 ; Zhang et al., 2000 ). Structural gene

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Ruigang Wu, Yi Wang, Ting Wu, Xuefeng Xu, and Zhenhai Han

abiotic stress. In plants, TFs play vital regulatory roles in abiotic stress responses by binding to the promoters of abiotic stress-responsive genes ( Gujjar et al., 2014 ; Jung et al., 2008 ; Umezawa et al., 2006 ). Plant MYB TFs, which act as the

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Robert J. Griesbach, Ronald M. Beck, John Hammond, and John R. Stommel

mutation. Anthocyanin structural gene transcription requires the expression of at least one of each of three distinct transcription factor families: MYC, MYB, and WD40 ( Griesbach, 2005 ). The WD40 family encodes proteins characteristic of the β

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John R. Stommel, Gordon J. Lightbourn, Brenda S. Winkel, and Robert J. Griesbach

developmental-specific expression exhibited by anthocyanin structural genes is controlled by regulatory genes. A common set of proteins, comprised of MYB and bHLH MYC proteins plus WD40 repeat proteins, interact to form a regulatory complex that controls

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Qianqian Shi, Xiaoxiao Zhang, Xiang Li, Lijuan Zhai, Xiaoning Luo, Jianrang Luo, Lixia He, Yanlong Zhang, and Long Li

differential regulation of enzymes involved in anthocyanin biosynthesis, which has been extensively studied ( Espley et al., 2007 ; Koes et al., 2005 ). Furthermore, the structural genes involved in anthocyanin biosynthesis are regulated by the R2R3-MYB, basic

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John R. Stommel and Judith M. Dumm

spectrophotometer (ND1000; NanoDrop Technologies, Wilmington, DE) and integrity and quantification confirmed using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Real-time PCR was used to compare flavonoid gene expression ( Chs , Dfr , Ans , Myb, Myc

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Yingtao Ma, Mengmeng Zhao, Hongxia Wu, Congying Yuan, Huiyun Li, and Yanzhao Zhang

, DFR , and UFGT , and the three transcription factor–encoding genes, including bHLH3 , MYB10.1 , and MYB10.2 , were determined for bagged peach pericarps and control peach pericarps. The expressions of these four structural genes were

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Xiaoying Dou, Jinrong Bai, Huan Wang, Ying Kong, Lixin Lang, Fang Bao, and Hongzhong Shang

). Quantitative real-time PCR analysis of the expression levels of the anthocyanin biosynthesis genes ATCHS , ATCHI , DFR , and ANS ; the regulatory MYB TF gene ATPAP1 ; and the bHLH gene GL3 revealed that the average expression level of DFR in the