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The coat protein (CP) gene of lettuce big-vein associated virus (LBVaV) in sense or antisense orientation in a binary vector pBI121 was transformed via Agrobacterium tumefaciens (Smith and Towns.) Conn. mediated transformation into lettuce (Lactuca sativa L.) to generate LBVaV-resistant lettuce. Nineteen T1 lines were produced; five to 10 plants of each T1 line were inoculated with LBVaV using Olpidium brassicae (Wor.) Dang.; and LBVaV was not detected in eight plants derived from six lines. T2 seedlings from the eight plants were tested for LBVaV resistance, and one line (line A-2) with the CP gene in antisense orientation was resistant to LBVaV while the other lines were susceptible. The transgenic line A-2 was also resistant to mirafiori lettuce virus (MiLV) and big-vein expressions regardless of the presence or absence of LBVaV.

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58 Hayes, R.J. Wintermantel, W.M. Nicely, P.A. Ryder, E.J. 2006 Host resistance to Mirafiori lettuce big-vein virus and l ettuce big-vein associated virus and virus sequence diversity and frequency in

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by Mirafiori lettuce big-vein virus ), and tipburn (physiological disorder) ( Davis et al., 1997 ). Data were analyzed using JMP 6.0.3 (SAS Institute, Cary, NC) and Tukey's honestly significant difference test. Disease resistance. All plants

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-indeterminate, short guides with no ability to climb, not adapted to direct harvest, 42 days to 50% bloom. Resistance: rust (All known races except 108), Bean common mosaic virus (Races controlled by the ā€œIā€ gene). PVP#: 200600224. Big Red (09351r)ā€” Breeder and

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, D.M., R.W. Robinson, and C. Jeffrey (eds.). Biology and utilization of the cucurbitaceae. Comstock Publishing Associates, Ithaca, NY Gilbert, R.Z. Kyle, M.M. Munger, H.M. Gray, S.M. 1994 Inheritance of resistance to watermelon mosaic virus in

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; 4 University of Florida, Gainesville, FL; 5 FDACS-DPI, Gainesville, FL Whitefly-transmitted Squash vein yellowing virus (SqVYV) was shown in the mid-2000s to cause a watermelon vine decline in southwest and west-central Florida. More recently

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