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Ryan N. Contreras and John M. Ruter

as the Philippine Islands for Old World species and Cuba for New World species, the former comprising a much larger group ( Moldenke, 1936 ). Callicarpa was previously placed in the Verbenaceae; however, it was recently transferred into Lamiaceae

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Roberto F. Vieira, Peter Goldsbrough, and James E. Simon

Molecular markers were used to assess genetic diversity in basil (Ocimum L. spp., Lamiaceae). Using randomly amplified polymorphic DNA (RAPD) analysis, 11 primers generated 98 polymorphic bands, ranging from 300 to 2,000 base pairs, that discriminated among 37 accessions across nine Ocimum spp. Means of genetic similarities within Ocimum spp. showed that the domesticated species, O. minimum L. (0.887), O. basilicum L. (0.769), and O. ×citriodorum Vis. (0.711) had highest similarity indices within species, while the nondomesticated, O. americanum L. (0.580), O. gratissimum L. (0.408), and O. kilimandscharicum Guerke (0.559) showed the lowest similarity. RAPD results indicated that O. minimum should not be considered a distinct species but rather a variety of O. basilicum. Consistent clusters among all but one of the O. ×citriodorum spp., all containing citral as the major constituent, were identified using bootstrap analysis. RAPD analysis was useful in discriminating among Ocimum spp., although within species resolution will require a higher number of polymorphic bands.

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Joseph Tychonievich and Ryan M. Warner

(Lamiaceae) from Argentina Bot. J. Linn. Soc. 141 483 490 Bentham, G. 1848 Labiatae 27 603 De Candolle A. Prodomus systematis naturalis regni vegetabilis Vol. 12 Treuttel et Wortz Paris, France Bentham, G. 1876 Labiatae 1160 1196 Bentham G. Hooker J.D. Genera

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Cynthia B. McKenney, Sandra A. Balch, Victor Hegemann, and Susan P. Metz

sage, a member of the Lamiaceae family, is native to areas characterized by rocky outcroppings, limestone prairies, floodplains, and hillsides. This herbaceous perennial is valued for its violet–blue flower spikes and informal growth habit ( Arnold

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Roger G. Fuentes-Granados, Mark P. Widrlechner, and Lester A. Wilson

The inheritance of five allozymes was studied in anise hyssop (Agastache foeniculum) by analyzing the progeny of controlled crosses. The loci studied [Cat-1, Got-2, Pgm-2, Tpi-1, and Tpi-2] were scored by using starch gel electrophoresis. Segregation analyses of families polymorphic at each of these loci support the following hypotheses: Cat-1 is controlled by a single gene with codominant alleles; Got-2 is controlled by a single gene with codominant alleles coding for dimeric protein products; Pgm-2 is controlled by a single gene with codominant alleles coding for monomeric proteins; and Tpi-1 and Tpi-2 are each controlled by a single gene with codominant alleles coding dimeric protein products. Distorted segregation ratios were observed in some families segregating for Got-2 and Pgm-2. No linkages were detected among any of the cosegregating loci.

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A. Estilai, A. Hashemi, and K. Truman

Edible chia seeds, purchased from the local markets in Guatemala, Mexico, and southern California, were used for species identification, chromosome counts, karyotype construction, and meiotic analyses. Plants raised from those seeds had ovate leaves, dense racemose inflorescences, pale-blue flowers, and were identified from herbarium specimens as Salvia hispanica L. Mitotic analyses of root tips from 50 plants showed 2n = 12—the lowest chromosome number in the genus. Chromosomes were small, ranging from 2 to 3.5 pm. One pair of chromosomes was metacentric (with the long arm: short arm ratio, r = 1.5), four pairs were submetacentric (r = 2.6 to 3.6), and one pair was telocentric (r = 12). Meiosis was regular and six bivalents were observed at metaphase I. Ring and rod bivalents averaged 1.53 ± 1.05 and 4.47 ± 1.05, respectively;

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Renee G. Nation, Jules Janick, and James E. Simon

The rate of outcrossing in basil [eight accessions of Ocimum basilicum L. and one accession purported to be O. kilimandscharicum Guerke (`Juicy Fruit')] was estimated using a purple seedling marker in `Dark Opal' (O. basilicum). There were two patterns of outcrossing: `Picollo' and four sources of `Cinnamon' basil had outcrossing rates between 19.9% and 32.8%, while `Lemon', PI 368699, PI 174284, and a Purdue selection named `Juicy Fruit' had outcrossing rates between 1.6% and 3.4%.

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F. Todd Lasseigne, Stuart L. Warren, Frank A. Blazich, and Thomas G. Ranney

Eight taxa of Salvia L., representing broad geographic origin and diversity within the genus, were grown under long day conditions for 36 d at 15-h days of 20, 25, 30, 35, or 40 °C and 9-h nights of 15 or 25 °C. Taxa of European origin displayed broader tolerance to high day temperatures (DTs) with the lowest relative reduction in growth and net photosynthesis (Pn) occurring at DTs 30 °C or greater compared with those native to North and South America. Salvia splendens Sell. ex Roem. & Schult. (scarlet sage) was particularly intolerant of high temperatures with all plants dying at days of 40 °C. All plants of S. nemorosa L.‘Ostfriesland’ (‘Ostfriesland’ wood sage), S. pratensis L. (meadow sage), and S. × sylvestris L. ‘Mainacht’ (‘May Night’ salvia) survived at days of 40 °C with no visual signs of injury, whereas all other taxa except S. splendens exhibited stunted, contorted growth with foliar chlorosis and necrosis at 40 °C. Day temperature exerted the primary effect on top growth, root growth, and Pn of all taxa. Night temperature effects were significant for some taxa but were of less importance than day temperature.

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Luping Qu and Mark P. Widrlechner

-pollination such as dioecy and self-incompatibility. However, many plant species vary between these two extremes in their reproductive biology ( Goodwillie et al., 2005 ). Prunella vulgaris L. (Lamiaceae), commonly known as selfheal, is a low-growing perennial

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Kelly J. Vining, Q Zhang, C.A. Smith, and T.M. Davis

We are using Mentha longifolia (Lamiaceae) as a diploid model species with relevance to the polyploid commercial mints ( Mentha L. spp.) and to the study of plant resistance to vascular wilt diseases. Verticillium wilt, incited by the fungus