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Kai-Shu Ling and Amnon Levi

, all other cucurbits, including bottlegourd [ Lagenaria siceraria (Mol.) Standl.], are vulnerable to ZYMV infection. Natural infection of bottlegourd by ZYMV has been reported in Hawaii ( Ullman et al., 1991 ), India ( Verma et al., 2004 ), and Serbia

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Jacob Mashilo, Hussein Shimelis, Alfred Odindo, and Beyene Amelework

Bottle gourd [ Lagenaria siceraria (Molina) Standl.] belongs to the Cucurbitaceae family. It is a diploid (2 n = 2 x = 22) vine crop widely grown in rural communities in South Africa ( Achigan-Dako et al., 2008 ; Beevy and Kuriachan, 1996 ). The

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Jeung-Sul Han* and Chang Kil Kim

A procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 carrying a binary vector pCAMBIA3301, which contains glufosinate ammonium-resistant (bar) and the reporter (gus) genes, is describe. Infection was the most effective (highest infection frequency and index) when explants were co-cultivated with Agrobacterium for 6-8 days on co-cultivation medium supplemented with 0.001-0.1 mg/L L-a-(2-aminoethoxyvinyl) glycine (AVG). Transgenic plants were obtained with frequencies of about 0.2% when the explants were cultured on selection medium (MS medium supplemented with 3.0 mg/L BAP, 0.5 mg/L AgNO3, 500 mg/L cefotaxime, 2.0 mg/L DL-phosphinothricin, 0.3% sucrose and 0.8% Plant Agar. A histochemical gus assay, PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progenies showed that the transgenes were inherited in a Mendelian fashion. To our knowlege, this study represents the first report for Agrobacterium-mediated transformation in bottle gourd, rootstock for watermelon and other cucurbit crops in many countries.

Open access

Nebahat Sari, Emily Silverman, Danny Reiland, and Todd C. Wehner

., 2007 ). There is a considerable interest in bottle gourd seeds because of their high nutritional quality, mainly in terms of protein and oil content ( Pradhan et al., 2013 ). Lagenaria siceraria can also be used as a rootstock for watermelon to

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Chandrasekar S. Kousik, Amnon Levi, Kai-Shu Ling, and W. Patrick Wechter

Bottle gourd [ Lagenaria siceraria (Mol.) Standl.], which belongs to the Cucurbitaceae family, is considered to be one of the earliest species of plants to be domesticated by humans. Bottle gourds are regularly grown and consumed in various parts

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Matthew A. Cutulle, Howard F. Harrison Jr., Chandresakar S. Kousik, Phillip A. Wadl, and Amnon Levi

Lagenaria siceraria (Mol.) Standl., a viny species of the Cucurbitaceae family, is thought to be among the first domesticated plant species ( Cutler and Whitaker, 1967 ). Its dry, mature fruit is used by people throughout the world for making

Open access

Asma Mohammed Saeed Al-Kubati, Baoshan Kang, Liming Liu, Aqleem Abbas, and Qinsheng Gu

cropping ( Colla et al., 2010 ). For cucurbits, the bottle gourd ( Lagenaria siceraria ) is an essential rootstock used to improve the disease resistance of grafted plants (also known as a scion), especially watermelon or melon ( Gaion et al., 2017

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Benjamín Moreno, Cristián Jacob, Marlene Rosales, Christian Krarup, and Samuel Contreras

. Lee, S. Ko, K. 2012 Development of fusarium wilt-resistant F1 hybrids of bottle gourd ( Lagenaria siceraria Standl.) for watermelon rootstocks. Cucurbitaceae 2012. Proc. Xth EUCARPIA Mtg. Genet. Breeding Cucurbitaceae, Antalya, Turkey, 15–18 Oct. 2012

Open access

Abigail Attavar, Lydia Tymon, Penelope Perkins-Veazie, and Carol A. Miles

, depending on the cultivar and on the number of seeds purchased ( Lewis et al., 2014 ). The rootstock species that are most commonly used for watermelon grafting are bottle gourd [ Lagenaria siceraria (Mol.) Standl.], interspecific hybrids between Cucurbita

Open access

Pinki Devi, Penelope Perkins-Veazie, and Carol A. Miles

-resistance. The most commonly used rootstocks for watermelon grafting are Lagenaria siceraria (bottle gourd), Cucurbita moschata (winter squash or pumpkin), and C. moschata × C. maxima (interspecific squash hybrid). Although watermelon grafted onto