Search Results

You are looking at 1 - 10 of 106 items for :

  • Illumina sequencing x
Clear All

., 2012 )]. RNA-seq is an important tool for obtaining transcripts from certain plant tissues under specific physiological conditions or at specific developmental stages ( Strickler et al., 2012 ; Wang et al., 2009 ). Illumina (San Diego, CA) sequencing

Free access

and their potential targets in P. orientalis , as well as a corresponding metabolic pathway involved in stress response. We constructed an independent P. orientalis sRNA library by high-throughput Solexa Illumina sequencing. The conserved miRNAs

Open Access

using a Covaris S220 ultrasonicator (Covaris, Inc., Woburn, MA) and following the standard protocol of the Illumina MiSeq Reagents kit v2 (Illumina, San Diego, CA). Illumina sequencing was conducted on the MiSeq system (Illumina) with 150 bp paired

Free access

/280 ratio between 1.8 and 2.0 as well as an RNA integrity number >8.0 were used for cDNA library preparation. Six small RNA (sRNA) libraries were constructed and subjected to single-end sequencing using the Illumina HiSeq 2500 platform (Illumina, San Diego

Free access

-normalized cDNA libraries synthesized from leaf and stem tissues of garlic. Sun et al. (2012) completed Illumina (San Diego, CA) sequencing of cDNAs from garlic buds to generate over 127,000 assembled sequences, of which about one-third showed significant

Free access

preparation consisted of: DNA sonication [Bioruptor XL (BR_XL); Diagenode, Denville, NJ]; DNA strand end repair; addition of an adenine base to the 3′ ends; ligation of a custom Illumina ® sequencing adapter (Illumina, San Diego, CA) containing a 4-bp barcode

Free access

accession RB14 ‘Timperley Early’ was one of eight Illumina ® sequencing libraries that were pooled and sequenced in one lane with the Illumina ® Genome Analyzer II at the Oregon State University Center for Genome Research and Biocomputing (CGRB). Each

Free access

-Madison Biotechnology Center for the 16S Metagenomic Sequencing Library Preparation Protocol, Part # 15044223 Rev. B (Illumina Inc., San Diego, CA). Modifications to the protocol included amplification of the 16S rRNA gene V3/V4 variable region with fusion primers

Free access

FLX (Roche, Penzberg, Germany), and Illumina Genome Analyzer (Illumina, San Diego, CA) can facilitate high-throughput genome sequencing of both noncoding and coding regions, including large-scale resequencing in well-characterized species or de novo

Free access

) for future DNA extraction and identification of all Phytophthora species present through Illumina MiSeq (Illumina, San Diego, CA) sequencing. Illumina MiSeq is a high-throughput sequencing platform that allows for identification of individual species

Open Access