In this study, the nuclear ribosomal DNA internal transcribed spacer region (ITS) of six Rubus cultivars were sequenced, then compared with sequences of three Rubus species in Genbank. DNA sequencing revealed little genetic variation among blackberry cultivars, but ably revealed distinctions between blackberry and red raspberry genotypes. Analysis by maximum-parsimony and pairwise genetic distances confirmed the small variation among blackberry cultivars. The resulting sequences were analyzed for useful restriction sites and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted on a total of six cultivars to establish genetic variation. Digests were difficult to interpret due to heterogeneity at restriction sites.
Iron-deficiency symptoms are observed on some genotypes of potato (Solanum tuberosum L.) grown on high-pH and calcareous soils. seven potato cultivars differing in response to iron deficiency chlorosis (FeDC) were grown on high-pH (8.1), calcareous (38% calcium carbonate equivalent in surface 20 cm) and silty clay soil in the field (Beka'a Valley, Lebanon), to determine the effects of FeDC on tuber yield of cultivars sprayed with Fe. A significant interactions between cultivars and Fe spray treatment were noted for visual FeDC ratings and tuber yield. Even though only slight FeDC was noted on some cultivars receiving no Fe spray, tuber yields were significantly increased when sprayed with Fe. Some cultivars with moderate FeDC ratings did not show a significant increase in yield when sprayed with Fe while other cultivars did. Sprayed cultivars generally produced higher tube; yields than unsprayed ones. Indicating that Fe-deficiency chlorosis in the Mediterranean region may be a serious limitation to potato tuber yield.
repeat analysis was also used to authenticate several populations of D. officinale ( Shen et al., 2006 ) and to evaluate the genetic diversity of 31 Dendrobium species ( Wang et al., 2009 ). The ITS region (ITS1-5.8S-ITS2) is highly repeated, up to
Caribbean. Cluster B, which had its greatest diversity in the Upper Amazon region according to diversity estimates ( Tables 2 and 4 ), was the most geographically diverse and predominant group, extending from Central America in the north to the Guianas
total sales, except in the Pacific region (13%). In some regions, forward-contracting accounted for a significantly higher share of total sales, perhaps indicating greater aversion to market risk. The Mountain region stands out for its high level of
affects its quality and acceptance. Table 3 shows the results obtained in multiple regression analyses. This table shows that in the northern region of the Antioquia, the DM exhibits a direct proportional relation concerning time predictor variables and
Horseradish is a hardy perennial that is a member of the Brassicaceae family and is grown for its white, thickened, and pungent roots ( Rubatzky and Yamaguchi, 1997 ). The large primary roots are fleshy with a smooth or corky surface. The intense
Ornamental sunflowers have become increasingly popular as cut flowers the world over ( Blacquière et al., 2002 ; Devecchi, 2005 ). Despite its popularity as a cut flower, growers of cut sunflowers have long complained about the susceptibility of
has received minimal attention in ornamental species, repeated isolations of this fungus from dogwood roots and the crown region in field plantings suggest a need to evaluate its impact on flowering dogwood seedlings. Common cultural practices used in
identification in cases of fish mislabeling, among others ( Cywinska et al., 2006 ; Di Pinto et al., 2015 ; Hebert et al., 2004 ; Rolo et al., 2013 ). In plants, the use of the COI region is not recommended for DNA barcoding studies due to its low