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James F. Harbage and Dennis P. Stimart

Involvement of pH and IBA on adventitious root initiation was investigated with Malus domestica Borkh. microcuttings. The pH of unbuffered root initiation medium (RIM) increased from 5.6 to 7 within 2 days. Buffering with 2[N-morpholino] ethanesulfonic acid (MES) adjusted to specific pHs with potassium hydroxide prevented pH changes and resulted in a 2-fold higher root count at pH 5.5 compared to pH 7 or unbuffered medium. As pH decreased, lower concentrations of IBA were required to increase root counts. Colorimetric measurement of IBA in buffered RIM showed greater IBA loss and higher root count were associated with lower pH levels in all cultivars. This suggests that IBA loss from RIM depends on medium pH, which affects root count. Root count differences between easy-to-root through difficult-to-root cultivars were not consistent with amount of IBA loss from RIM. Cultivar differences in root count could not be explained solely by IBA loss from RIM.

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Benjamin E. Deloso, Anders J. Lindström, Frank A. Camacho, and Thomas E. Marler

, and 100% for the 3, 8, or 30 mg·g −1 plants. Table 1. The response of Zamia furfuracea and Zamia integrifolia stem cuttings to indole-3-butyric acid concentrations of 0, 3, 8, 16, or 30 mg·g −1 . N = 25. The Z. integrifolia IBA study lasted 356

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Simone da Costa Mello, Jéssika Angelotti-Mendonça, Lucas Baiochi Riboldi, Luigi Tancredi Campo Dall’Orto, and Eduardo Suguino

1. Effect of indole-3-butyric acid (IBA) concentration (0, 30, 60, and 90 mg·L −1 ) and cuttings type (softwood and semihardwood) on cutting survival (CS), rooting, number of roots (NOR), dry weight of roots (DWR), and root length (RL) of ‘Yabukita

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Benjamin D. Taylor and Benjamin K. Hoover

least square means data for wall germander cuttings receiving indole-3-butyric acid (IBA) treatments of 0, 1000, or 3000 ppm applied as a foliar spray or talc dip ( n = 7). Primary root count . In the Fall 2016 experiment, there was a significant

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Bryan J. Peterson, Gregory J.R. Melcher, Ailish K. Scott, Rebecca A. Tkacs, and Andrew J. Chase

cuttings from the first collection date that received 15,000 mg·L −1 K-IBA were diminished. Fig. 1. Mean rooting percentages, root ratings, and root lengths of sweetgale in response to potassium salt of indole-3-butyric acid (K-IBA) concentration and

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Todd J. Rounsaville, Darren H. Touchell, Thomas G. Ranney, and Frank A. Blazich

.59 + 0.064 (IBA), r 2 = 0.25, P < 0.05. Vertical lines = ± 1 se . IBA = indole-3-butyric acid. Regression analysis performed on ex vitro rooting data revealed no significant linear or quadratic trends. The highest concentration of K-IBA (41.4 μM

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Xiuli Shen, Guochen Yang, and Zhongge (Cindy) Lu

on MS medium supplemented with 5 μM 6-benzylaminopurine (BAP). ( G ) Single root formation on MS medium supplemented with 5 μM indole-3-butyric acid (IBA). ( H ) Double root formation on the same medium. ( I ) multiple root formation on the same

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Xiuli Shen, William S. Castle, and Frederick G. Gmitter Jr

obtained on Murashige and Skoog (MS) medium ( Murashige and Skoog, 1962 ) supplemented with 6-benzylaminopurine (BA) at 0 to 35.6 μM and root induction on MS medium supplemented with indole-3-butyric acid (IBA) from 4.3 to 17.4 μM ( Shen et al., 2009b

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Jalil Dejampour, Islam Majidi, Solmaz Khosravi, Sevil Farhadi, and Atena Shadmehr

intensity and subsequent 8-h dark condition in a growth chamber at 23 ± 1 °C. They were sub-cultured every 3 weeks. Table 1. Proliferation treatments containing different concentrations of benzyl amino purine (BAP) and indole butyric acid (IBA) for the HS314

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Milorad Vujičić, Aneta Sabovljević, Jasmina Šinžar-Sekulić, Marijana Skorić, and Marko Sabovljević

among treatment according to least significant difference ( lsd ) test ( lsd 0.05 =11.74, P < 0.05) IBA = indole-3-butyric acid; BA = N 6 -benzyladenine; MS = Murashige and Skoog. Auxins and cytokinins have basic functions in the regulation of normal