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Keri D. Jones, Sandra M. Reed, and Timothy Rinehart

Dichroa febrifuga (Hydrangeaceae) is an evergreen shrub with metallic blue fruit that persists throughout winter. Phylogenetic studies have indicated that D. febrifuga is closely related to Hydrangea macrophylla. The objective of this study was to produce hybrids between these two ornamental species. Reciprocal crosses were made between D. febrifuga and both diploid and triploid cultivars of H. macrophylla. Two-thirds of the seed capsules produced from these hybridizations were allowed to mature on the plant, after which time seed were collected and sown in a greenhouse. Embryo rescue was used with the remaining seed capsules. Ovules were excised from ovaries about 9 weeks after pollination and plated on Gamborg's B-5 media with 2% sucrose. Both germination techniques resulted in vigorous seedlings. Hybridity was verified in plants obtained from ovule culture using simple sequence repeat (SSR) markers. Reciprocal hybrids were obtained using both diploid and triploid forms of H. macrophylla. This study confirms the close relationship between D. febrifuga and H. macrophylla and provides valuable material for future breeding efforts.

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William G. Hembree, Thomas G. Ranney, Nathan P. Lynch, and Brian E. Jackson

hybrids and cultivars has continued since then. The taxonomic history of Deutzia has seen it placed within both the Saxifragaceae and the Philadelphaceae families, although it is currently accepted as a member of Philadelpheae within Hydrangeaceae

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Sandra M. Reed

Oakleaf hydrangea ( Hydrangea quercifolia Bartr.; family Hydrangeaceae Dumort.) is an ornamental shrub that is native to the southeastern United States ( McClintock, 1957 ). Most plants grow 2 m or taller in height with an equal to wider spread

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Sandra M. Reed and Lisa W. Alexander

Oakleaf hydrangea ( Hydrangea quercifolia Bartr.; Hydrangeaceae) is a woody shrub native to the southeastern United States, where it grows in calcareous soils in the understory of open woodlands ( McClintock, 1957 ). There are ≈70 species in the

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Timothy A. Rinehart, Brian E. Scheffler, and Sandra M. Reed

The genus Dichroa Lour., which is a member of the Hydrangeaceae, includes 12 species native to eastern Asia and adjacent islands ( Shumei and Bartholomew, 2001 ). Dichroa febrifuga Lour. is one of the 50 fundamental herbs in Chinese herbology

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Sandra M. Reed, Keri D. Jones, and Timothy A. Rinehart

Dichroa febrifuga is an evergreen native to Nepal, southern China, and Southeast Asia, and it is cultivated both as an ornamental and a medicinal plant ( Hinkley, 2005 ; Shumei and Bartholomew, 2001 ). A member of the Hydrangeaceae, it grows 1

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Joshua H. Kardos, Carol D. Robacker, Michael A. Dirr, and Timothy A. Rinehart

introduced new wild-collected H. macrophylla germplasm (personal communication). Although interspecific and intergeneric hybridizations have been attempted within the Hydrangeaceae, most of the resultant hybrids were weak, sterile, or had reduced fertility

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Ming Cai, Ke Wang, Le Luo, Hui-tang Pan, Qi-xiang Zhang, and Yu-yong Yang

that are ornamentally attractive and commonly cultivated ( Kudo et al., 2008 ). Bigleaf hydrangea ( H . macrophylla ) is the most commonly cultivated member of the Hydrangeaceae family and is native to southern China and Japan ( McClintock, 1957

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Youping Sun, Genhua Niu, Haijie Dou, Christina Perez, and Lisa Alexander

Hydrangea and allied genera of tribe Hydrangeeae (Hydrangeaceae) Bot. J. Linn. Soc. 137 139 187 Isaac, R.A. Johnson, W.C. 1975 Collaborative study of wet and dry ashing techniques for the

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Qiang Liu, Youping Sun, Genhua Niu, James Altland, Lifei Chen, and Lijuan Jiang

Asia. Hydrangea macrophylla (‘Smhmtau’ and ‘Smnhmsigma’) is a species of flowering plant in the family of Hydrangeaceae native to Japan. Parthenocissus quinquefolia , native to eastern and central North America, is a species of flowering plant in the