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Maedza K. Vuwani, Mpumelelo Nkomo, Wonder Ngezimana, Nokwanda P. Makunga and Fhatuwani N. Mudau

were kept in sealed glass vials and placed in the dark at 2 to 8 °C until further use (LC–MS). For gas chromatography linked to mass spectrometry (GC–MS), samples consisting of a single leaf per replication per plant were picked 24 h before being tested

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Shao-chang Qin, Juan-ling Li, Abdul Kareem and Yong Wang

strong surface of a fiber in an infusion needle ( Pragst, 2007 ). Because HS-SPME is nonpolluting to fiber-contrasted and DI-SPME, it was eventually chosen over the SD and SE methods. Using gas chromatography-mass spectrometry (GC-MS) has the advantage of

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Jian-rong Feng, Wan-peng Xi, Wen-hui Li, Hai-nan Liu, Xiao-fang Liu and Xiao-yan Lu

cultivated in Xinjiang, China were identified and determined using HP-SPME with GC-MS. The differences in the aroma composition and content among cultivars were analyzed and the main characteristic aroma volatiles in these cultivars were ascertained by using

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Zhongshuai Gai, Yu Wang, Jutang Jiang, Hui Xie, Zhaotang Ding, Shibo Ding and Hui Wang

the metabolite profiles using GC-MS and LC-MS/MS. This study not only established an evaluation system for tea germplasm appraisal but also promoted germplasm utilization for tea breeding. Materials and Methods Plant materials. The tea germplasm

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Ying Kong, Ming Sun, Hui-tang Pan and Qi-xiang Zhang

; PerkinElmer) coupled to a MS (Clarus 600T; PerkinElmer). The GC was fitted with a DB-5MS fused-silica capillary column (30 m × 0.25 mm × 0.25 mm; Agilent Technologies, Santa Clara, CA). The GC oven temperature was programmed at 40 °C for 2 min, then the

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Chaowei Song, Qi Wang, Jaime A. Teixeira da Silva and Xiaonan Yu

scent of 21 herbaceous peony cultivars identified 34 volatile compounds using headspace solid-phase microextraction (HS-SPME) coupled with GC-MS ( Feng et al., 2016 ). In related research of tree peony (section Moutan of the genus Paeonia ), Li et al

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Mostafa Farajpour, Mohsen Ebrahimi, Amin Baghizadeh and Mostafa Aalifar

1 m 2 plots in a sandy-loam soil. The results of soil analysis of the filed are present in Table 2 . Accessions were harvested at 10–20% flowering stage to obtain essential oils. For GC-MS analysis, the plants from the three replications were mixed

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Ying Kong, Jinrong Bai, Lixin Lang, Fang Bao, Xiaoying Dou, Huan Wang and Hongzhong Shang

chromatography–mass spectrometry (GC/MS) analysis. The tubes containing the volatiles were desorbed in an automatic thermal desorber (Turbo Matrix 650; PerkinElmer, Waltham, MA) at 260 °C for 10 min. Helium flow rate was 1.5 mL·min −1 . The desorbed volatile

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Xiaohua Yang, Susan K. Brown and Peter J. Davies

identified using GC-MS. Metabolism of [ 14 C]GA 12 was compared between dwarf and standard plants as well as self-rooted plants and plants on vigorous rootstocks. Materials and Methods Plant material. Vigorous apple shoots were collected in June 2011 from

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David R. Rudell, Sara Serra, Nathanael Sullivan, James P. Mattheis and Stefano Musacchi

analyzed daily for all LC-MS and GC-MS analyses. The response of a selection of mass spectral tags (MSTs) representing both identified and unidentified metabolites was monitored to assure consistent extraction and instrument performance throughout the