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Sanalkumar Krishnan, Kevin Laskowski, Vijaya Shukla, and Emily B. Merewitz

species. Therefore, evaluating the function of plant metabolites such as GABA that may promote the drought tolerance of perennial ryegrass and other turfgrasses is needed. GABA is a four-carbon non-protein amino acid conserved from prokaryotes to

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Tomohiro Okada and Yoh-ichi Matsubara

0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, sucrose 30 g, agar 8 g·L −1 (pH 5.8). The conidia were further subcultured (10 6 conidia/mL) in liquid Czapec-Dox media with or without addition of filter-sterilized amino acids (arginine, GABA; 0

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Yehoshua Saranga, David Rhodes, and Jules Janick

Abbreviations: EPM, embryo production medium; GABA, γ -aminobutyrate; PAR, photosynthetically active radiation. Journal paper no. 12,636 of the Purdue Univ. Agricultural Experiment Station. We thank A. Altman, Faculty of Agriculture, Hebrew Univ. of

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Zhou Li, Yan Peng, and Bingru Huang

recent years, a nonprotein amino acid, GABA, has been found to exhibit PGR effects due to its central roles in maintaining carbon and nitrogen balance and its involvement in carbohydrate and amino acid metabolism ( Barbosa et al., 2010 ; Bouché and Fromm

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Zhongjie Ji, James J. Camberato, Cankui Zhang, and Yiwei Jiang

plant growth and salinity tolerance. Other chemical or signaling molecule such as GABA and NO also demonstrated a role in regulating plant responses to salinity stress ( Ahmad et al., 2016 ; Liu et al., 2016 ; Wang et al., 2017 ; Ziogas et al., 2017

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Victor Gaba, Chassia Elman, Abed A. Watad, and Dennis J. Gray

Cotyledonary explants of melon (Cucumis melo L. cv. Galia) regenerate primordia and buds in vitro induced by benzyladenine. The anti-gibberellin ancymidol can stimulate the rate of regeneration on melon explants in the presence of benzyladenine. Concentrations of benzyladenine plus ancymidol that are individually ineffective can act synergistically to stimulate regeneration. Gibberellic acid reduces the rate of regeneration induced by benzyladenine or benzyladenine plus ancymidol. Chemical names used: N-(phenylmethyl)-1H-purine-6-amine (benzyladenine); alpha-cyclopropyl-alpha-(4-methoxy-phenyl)-5-pyrimidine methanol (ancymidol).

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A.A. Watad, K.G. Raghothama, M. Kochba, A. Nissim, and V. Gaba

Explant growth and shoot multiplication of Spathiphyllum and Syngonium were compared on agar-solidified medium and interfacial membrane rafts floating on liquid medium. After 25 d of culture, greater shoot multiplication and fresh mass gain were achieved by plant material grown on rafts. Shoot multiplication of Spathiphyllum and Syngonium on membrane rafts reached a maximum at 25 d, whereas the fresh mass increased throughout the culture period (40 d). The number of shoots of Spathiphyllum and Syngonium material grown on membrane rafts remained constant between 25 and 40 d of culture. The plants grown on membrane rafts also developed more roots.

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Sebahattin Curuk, G. Ananthakrishnan, Sima Singer, Xiaodi Xia, Chassia Elman, David Nestel, Selim Cetiner, and Victor Gaba

Hypocotyl explants of three cultivars of melon (Cucumis melo L.) (cvs. Revigal, Topmark and Kirkagac), and a cucumber (C. sativus L. cv. Taoz) rapidly directly regenerated multiple shoots on Murashige and Skoog medium augmented with 4.4 μm benzyladenine. Regeneration from the hypocotyl resulted in nearly 100% diploid shoots, whereas regeneration from the cotyledons resulted in 40% to 70% polyploid regenerants. Regeneration from cotyledon explants of melon cv. Revigal required light, whereas regeneration from hypocotyl explants of melon cv. Revigal occurred in both light and darkness. Direct regeneration also occurred from the hypocotyl of cucumber cv. Taoz in both light and darkness, even though cotyledonary explants did not regenerate buds or shoots under the same conditions. This is the first report of regeneration from the Cucumis genus producing a fully diploid plant population.

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D.J. Gray, E. Hiebert, C.M. Lin, K.T. Kelley, M.E. Compton, and V.P. Gaba

Agrobacterium-mediated transformation (AMT) was compared with a particle bombardment (PB) for stable transformation of `Eden Gem', a melon with high embryogenic potential. Pretreated cotyledonary explants were either wounded via particle bombardment prior to Agrobacterium infection or were bombarded with plasmid-coated gold microparticles, using a modified particle inflow-type gun. Although similar numbers of embryos initially were obtained with each method, most produced via AMT became abnormal, possibly due to the growth-regulatory effects of the antibiotic mefoxine, which was used to inhibit Agrobacterium. Stably transformed plants and progeny were obtained only with PB, as determined by detection of the NPTII gene in R0 plant by Southern hybridization and, in progeny, by PCR amplification.

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Hongmei Du, Zhaolong Wang, Wenjuan Yu, and Bingru Huang

differences between days of treatment for hybrid bermudagrass based on least significant difference test ( P = 0.05). The amino acids exhibiting decline during the early phase of drought stress (6 d) included serine (Ser), GABA, and isoleucine (Ile) ( Fig. 3A