min followed by 50% (v/v) bleach [2.5% (v/v) sodium hypochlorite solution] plus 2 drops of Tween-80 for 10 min and rinsing four times with sterile-distilled water. Explants were placed on a modified DKW medium ( Driver and Kuniyuki, 1984 ) supplemented
Driver and Kuniyuki Walnut medium (DKW) ( Driver and Kuniyuki, 1984 ) compared with MS, Gamborg, and Babi media. Among carbon sources, sucrose has been the most widely used in hemp micropropagation studies ( Boonsnongcheep and Pongkitwitoon, 2020 ; Lata
Branch tips (30 to 40 cm long) of adult black walnut were forced in a half-strength solution of Long and Preece medium (LP) salts (minus iron) plus 1 mM 8-hydroxyquinoline citrate (8-HQC). The resulting softwood shoots were surface-disinfested and cut into 1.5-cm-long nodal segments. Explants were placed on two media: Driver and Kuniyuki Walnut medium (DKW) or LP with four plant growth regulator combinations: 5 μM BA with 0.05 μM IBA, 10 μM BA, 1 nM TDZ, or 10 nM TDZ in a factorial arrangement. Gelrite was used as the gelling agent. Explants were transferred to fresh medium on days 1, 3, 5, and 7 after initiation, then weekly. Data recorded 60 days after culture initiation showed more and longer shoots and leaves, greater explant diameter, more green (living) tissue, and less exudation per explant on LP than on DKW. Greatest explant and shoot length were observed when the medium contained 10 nM TDZ. BA (10 μM) and LP were best for long-term maintenance of cultures
rinsed three times with sterile distilled water and cut as single nodes in sterile petri dishes. The DKW medium with vitamins ( Driver and Kuniyuki, 1984 ) (Product ID: D2470, PhytoTechnology, Shawnee Mission, KS) was supplemented with 3% sucrose and 0
axillary shoot development. Next, we used these PGR levels to compare axillary shoot proliferation in three nutrient salt formulations: WPM ( Lloyd and McCown, 1980 ), MS, and DKW medium ( Driver and Kuniyuki, 1984 ). For ‘Washington Navel’ orange only, we
groups: 1) broadleaf tree basal (BLT), 2) Driver and Kuniyuki walnut (DKW), 3) L&M and McCown have the same formulation and were designed for woody plants by Lloyd and McCown (1981 ), and 4) MS medium. MS (M0404) and DKW were chosen as benchmarks and
and Skoog 1962 )] has been widely used in studies of hemp micropropagation ( Monthony et al. 2021c ). Page et al. (2021 ) found that Driver and Kuniyuki Walnut medium [DKW ( Driver and Kuniyuki 1984 )] was more suitable for micropropagation of hemp
thidiazuron in DKW medium basal salts ( Driver and Kuniyuki, 1984 ) while López-Baez et al. (2000) used 2,4-D or 2,4,5-T (1 mg·L −1 ) and Kin or 2 Isopentyladenine (2-ip) (0.25 mg·L −1 ) in a modified Murashige and Skoog (MS) salts ( Murashige and Skoog
; Quoirin and Lepoivre, 1977 ), DKW ( Driver and Kuniyuki, 1984 ), and Woody Plant Medium (WPM; Lloyd and McCown, 1980 ) media were used in the second experiment. All media were supplemented with BAP 0.5 mg/L, on the basis of the previous results, sucrose 3
-white fluorescent lamps. Expt. 1: Media composition and phenolic binding agents. Effect of basal salt compositions and vitamins (MS, half-strength MS, WPM, Blaydes, and DKW) in factorial combination with PBAs (control, 1 g·L −1 AC, or 1 g·L −1 PVP) was examined