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Eliezer Louzada, Omar Vazquez, Sandy Chavez, Mamoudou Sétamou, and Madhurababu Kunta

Candidatus Liberibacter asiaticus (CLas), the supposed causal agent of Huanglongbing (HLB), is a deadly bacterial pathogen responsible for devastation of the Florida citrus industry. CLas has been detected in nearly all citrus-producing areas in

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Ed Etxeberria, Pedro Gonzalez, Ariel Singerman, and Timothy Ebert

Dis. 97 1235 1243 Aritua, V. Achor, D. Gmitter, F.G. Albrigo, L.G. Wang, N. 2013 Transcriptional and microscopic analysis of citrus stem and root response to Candidatus Liberibacter asiaticus infection PLoS One, doi: 10.1371/journal.pone.0073742

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Ute Albrecht and Kim D. Bowman

until recently ( Sechler et al., 2009 ). Three Candidatus spp. of the pathogen are currently known. The most widespread Asian species, Ca . Liberibacter asiaticus, is found in all HLB-affected countries except Africa. The African species, Ca . L

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Ed Stover and Greg McCollum

isolation and Candidatus Liberibacter asiaticus quantification. Nucleic acid was extracted from citrus leaf laminar discs (3 mm diameter, ≈11 mg) using REDExtract-N-Amp (Sigma, St. Louis, MO) according to the manufacturer's instructions. Copy number of CLas

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Ed Stover, David G. Hall, Robert G. Shatters Jr., and Gloria A. Moore

following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing huanglongbing in Florida Plant Sci. 175 291 306 Ammar, E.D. Walter, A.J. Hall, D.G. 2013 New excised-leaf assay method to test inoculativity of Asian citrus psyllid

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Madhurababu Kunta, John V. da Graça, Nasir S.A. Malik, Eliezer S. Louzada, and Mamoudou Sétamou

number of samples of each type from the cultivars was random and a completely randomized design was used in the analysis. Table 1. Different plant parts used in the quantification of Candidatus Liberibacter asiaticus by quantitative polymerase chain

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Ute Albrecht, David G. Hall, and Kim D. Bowman

.S. Exteberria, E. Wang, N. Folimonova, S.Y. Chung, K.R. Albrigo, L.G. 2010 Sequence of anatomical symptom observations in citrus affected with huanglongbing disease Plant Pathol. J. 9 56 64 Albrecht, U. Bowman, K.D. 2009 Candidatus Liberibacter asiaticus and

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E.W. Stover and T.G. McCollum

analysis of variance, so means were compared using Kruskal-Wallis nonparametric analyses. Results and Discussion Candidatus Liberibacter asiaticus. Of the 90 samples of each tissue type analyzed for the presence of CLas, 88% of mature leaves, 69% of flowers

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Shahrzad Bodaghi, Bo Meyering, Kim D. Bowman, and Ute Albrecht

associated with Candidatus Liberibacter asiaticus (CLas), a phloem-limited bacterium ( Garnier et al., 1984 ), which has not yet been successfully grown in pure culture ( Merfa et al., 2019 ), and so remains elusive as the undisputed causal agent. Typical

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Shahrzad Bodaghi, Gabriel Pugina, Bo Meyering, Kim D. Bowman, and Ute Albrecht

Candidatus Liberibacter asiaticus (CLas), which is transmitted by the Asian citrus psyllid Diaphorina citri , a small insect that feeds on the phloem of citrus trees ( Halbert and Manjunath, 2004 ; Hall et al., 2013 ). Most commercially important citrus