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Douglas V. Shaw, John Hansen and Greg T. Browne

One hundred-eighty six strawberry genotypes from the Univ. of California strawberry (Fragaria ×ananassa Duch.) breeding program were evaluated for resistance to Phytophthora cactorum Schroet. in trials conducted over 6 years; 60 of these genotypes were tested in 2 years or more. Mother plants of each genotype were grown in a propagation nursery beginning in June, and runner plants were set into soil infested with inoculum from a mix of four P. cactorum isolates in August or September of the same year. Runner plants of each genotype were harvested from the inoculated nursery, transferred to a fruiting field location, and evaluated for disease symptoms during the winter and spring following inoculation using a disease severity score. Significant variation for the disease severity score was detected due to years, genotypes, and their interaction. Differences among genotypes were responsible for 60.6% of the phenotypic variance, whereas years and year × genotype interactions contributed relatively little to this variance, 8.2% and 9.3%, respectively. A separate analysis conducted using a balanced subset of six cultivars that were present in all trial years detected variance components due to years and year × genotype interaction slightly smaller than those estimated for the complete trial, 5.0% and 3.9%, respectively. These results highlight the utility of the screening system and suggest that stable resistance to P. cactorum is obtainable in California strawberry breeding populations and production systems.

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J.M. Quintana, H.C. Harrison, J. Nienhuis, J.P. Palta, K. Kmiecik and E. Miglioranza

To understand the genetics that control pod Ca concentration in snap beans, two snap bean (Phaseolus vulgaris L.) populations consisting of 60 genotypes, plus 4 commercial cultivars used as checks, were evaluated during Summers 1995 and 1996 at Hancock, Wis. These populations were CA2 (`Evergreen' × `Top Crop') and CA3 (`Evergreen' × `Slimgreen'). The experimental design was an 8×8 double lattice repeated each year. No Ca was added to the plants grown in a sandy loam soil with 1% organic matter and an average of 540 ppm Ca. To ensure proper comparison for pod Ca concentration among cultivars, only commercial sieve size no. 4 pods (a premium grade, 8.3 to 9.5 mm in diameter) were sampled and used for Ca extractions. After Ca was extracted, readings for Ca concentration were done via atomic absorption spectrophotometry. In both populations, genotypes and years differed for pod Ca concentration (P = 0.001). Several snap bean genotypes showed pod Ca concentrations higher than the best of the checks. Overall mean pod Ca concentration ranged from a low of 3.82 to a high of 6.80 mg·g-1 dry weight. No differences were detected between the populations. Significant year×genotype interaction was observed in CA2 (P = 0.1), but was not present in CA3. Population variances proved to be homogeneous. Heritability for pod Ca concentration ranged from 0.48 (CA2) to 0.50 (CA3). Evidently enhancement of pod Ca concentration in beans can successfully be accomplished through plant breeding.

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Harbans L. Bhardwaj and Anwar A. Hamama

Tepary bean (Phaseolus acutifolius A. Gray), a native of southwestern U.S., is a promising plant for crop diversification and for production in short rotations with wheat. However, protein and mineral concentrations in tepary bean seed produced outside the southwestern U.S. are largely unknown. We evaluated concentrations of protein and various minerals in seed produced by eight tepary bean genotypes planted at three different dates each during 1997 and 1998 at Ettrick, Virginia. Significant year × planting date and year × genotype interactions existed for protein and other traits. Protein and zinc concentrations increased and calcium concentrations decreased with later plantings during both years. Mid-June planting had 14% higher protein concentration (24.5%) than late-May planting (21.4%) and mid-July planting had 6% higher protein concentration (25.9%) than mid-June planting. Color of seedcoat was not associated with concentrations of protein or minerals. The average concentrations of boron, calcium, copper, iron, potassium, magnesium, manganese, phosphorus, sulfur, and zinc (mg/100g) were: 1, 184, 1, 11, 1531, 192, 3, 451, 311, 4, respectively. Tepary bean seeds contained 24% protein as compared to reported average values of 22.3% in navy, 22.5% in red kidney, and 20.9% in pinto bean. The average iron concentration (mg/100g) in tepary bean seed (10.7) was higher than that in navy (6.4), red kidney (6.7), and pinto (5.9) bean. Based on protein and mineral concentrations tepary bean seed compared well with seeds of navy, red kidney, or pinto bean.

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Javier Fernandez-Salvador, Bernadine C. Strik, Yanyun Zhao and Chad E. Finn

’ ( Table 5 ). There was a significant year × genotype interaction on each fruit quality characteristic measured at harvest ( Table 5 ). ‘Obsidian’ and ORUS 2635-1 produced the largest fruit, particularly in 2013, whereas ‘Onyx’ produced among the smallest

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Ossama Kodad and Rafel Socias i Company

because most genotypes present kernel weights higher than 1 g, considered an objective in almond breeding programs. In addition to the year-to-year fluctuation of these physical traits, there is a significant year × genotype interaction ( Table 3