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Kaitlin J. Palla, Rochelle R. Beasley, and Paula M. Pijut

for variety testing. In vitro propagation of common persimmon would be valuable for the development of commercial rootstocks for the nursery industry. The objective of this study was to develop an in vitro propagation protocol for four mature cultivars

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Rochelle R. Beasley and Paula M. Pijut

immature embryo at seed set ( Vandstone and LaCroix, 1975 ), and complex stratification and germination requirements ( Benedict and David, 2003 ). In vitro culture is a feasible tool for conserving, propagating, and genetically improving threatened and

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Jessica D. Lubell-Brand, Lauren E. Kurtz, and Mark H. Brand

et al., 2002 ). Micropropagation also requires substantially fewer mother plants to be maintained compared with traditional stem cutting propagation, and in vitro cultures can be stored for longer in a smaller area than mother plants. There are few

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Freddi A. Hammerschlag, Ghazala Hashmi, Robin Huettel, Dennis Werner, and David Ritchie

134 WORKSHOP 20 Reevaluating Tissue Culture Techniques for Generating Useful Variation and for in Vitro Screening

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Cristian Silvestri, Gianmarco Sabbatini, Federico Marangelli, Eddo Rugini, and Valerio Cristofori

adulteration of commercial products cannot be excluded, and morphological, histological, and molecular differentiations are necessary ( Potterat 2010 ; Sze et al., 2008 ; Zhang et al., 2001 ). Large-scale propagation of woody plant species through in vitro

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W.L. Chen and D.M. Yeh

contamination ( Kunisaki, 1980 ). Internal contamination can be minimized or eliminated using antibiotics incorporated into the culture media ( Kneifel and Leonhardt, 1992 ) or with pretreatment of explants before in vitro culture ( Kritzinger et al., 1998

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Alexandre Bosco de Oliveira, Wagner A. Vendrame, and Luciana Cardoso Nogueira Londe

). In jatropha, in vitro seed cryopreservation protocols have been developed, but some problems related to this procedure have been reported, such as decrease in seed germination percentage ( Salomão et al., 2016 ), low development of explants in vitro

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Patrick J. Conner

of artificial pollination and pollen storage protocols. Germination tests have generally been considered to be the best in vitro indicator of pollen usefulness ( Galleta, 1983 ). The in vitro germination test assesses the viability of a pollen sample

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J. Kevin Parris, Darren H. Touchell, Thomas G. Ranney, and Jeffrey Adelberg

. In vitro propagation procedures may be an efficient means for rapid, large-scale production. Previous micropropagation studies on Magnolia sp. focused on conservation efforts and included M. acuminata var. cordata (Michx.) Sarg. ( Merkle and

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José R. Bautista-Aguilar, Lourdes G. Iglesias-Andreu, Jaime Martínez-Castillo, Marco A. Ramírez-Mosqueda, and Matilde M. Ortiz-García

strategies for the conservation of plant germplasm is based on the use of plant tissue culture techniques to help establish an in vitro germplasm bank ( Kendon et al., 2017 ). In vitro conservation in the medium term, allows conserving aseptically, in a small