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Jack E. Staub, Philipp W. Simon, and Hugo E. Cuevas

source from which they may develop elite germplasm possessing high levels of β-carotene as a natural source of vitamin A to the human diet (i.e., improve nutritional value of cucumber). This germplasm is fixed for genes controlling the expression of β

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John P. Navazio and Philipp W. Simon

Three orange-mesocarp derivatives of the xishuangbannan cucumber (Cucumis sativus L. var. xishuangbannanesis Qi et Yuan), P100, P101, and P104; and NPI (P105), an unrelated cucumber (Cucumis sativus L.) reported to have orange flesh, were selected as parents for a diallel experiment to evaluate inheritance of orange cucumber mesocarp pigment over 3 years. Visual color intensity and carotenoid content were closely related. A preponderance of additive genetic effects for cucumber mesocarp pigmentation was observed in grade size 2 fruit (immature fruit used for pickling). Both additive and nonadditive genetic effects were important in grade size 4 fruit (mature). Years and yea× genotype interactions were highly significant for pigmentation of size 2 fruit, indicating the importance of environment on the expression of pigmentation in this size class. In contrast, color development was stable among years for size 4 fruit. P104 exhibited high general combining ability (GCA) estimates for size 4 fruit pigmentation across years, while P101 had high GCA estimates for size 2 fruit. The diallel analysis illustrated high fruit carotene content of parents per se. However, most hybrid combinations of the diallel reduced carotenoid content relative to parents, indicating both dominance for low carotenoid content for both fruit sizes and lack of genetic complementation among parents to enhance fruit color. Genetic control of pigmentation in size 2 fruit appeared to be independent of that for size 4 fruit.

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John R. Stommel, Judith A. Abbott, and Robert A. Saftner

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Thomas C. Koch and Irwin L. Goldman

Carotenoids and tocopherols are health-functional phytochemicals that occur in a wide range of fruit and vegetable crops. These two classes of compounds are synthesized from a common precursor, geranyl-geranyl pyrophosphate, and are typically analyzed separately via high-performance liquid chromatography (HPLC) techniques. Because carotenoids and tocopherols are present in many edible horticultural crops, it would be advantageous to measure them simultaneously in plant tissues. Herein we report a one-pass reverse-phase HPLC method for extraction and analysis of carotenoids and tocopherols in carrot that can be extended to other high-moisture plant organs. Elution times ranged from 5 minutes for α-tocopherol to 24 minutes for β-carotene. This method improves the efficiency of analyzing these compounds by up to 50%, and should increase the efficiency of assessing carotenoid and tocopherol profiles in horticultural crops.

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Muttalip Gündoğdu, Tuncay Kan, and Mustafa Kenan Gecer

phenolic content is found to be correlated with reduced incidences of heart diseases in epidemiological studies ( Criqui and Ringel, 1994 ; Renaud and de Lorgeril, 1992 ). The anticarcinogenic and antioxidant properties of vitamins A, C, E, β-carotene, and

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Silver Tumwegamire, Regina Kapinga, Patrick R. Rubaihayo, Don R. LaBonte, Wolfgang J. Grüneberg, Gabriela Burgos, Thomas zum Felde, Rosemary Carpio, Elke Pawelzik, and Robert O.M. Mwanga

for decades ( Ezell and Wilcox, 1958 ; Hagenimana et al., 1999 ; Purcell, 1962 ; Purcell and Walter, 1968 ), and currently OFSP is considered a complementary food approach to alleviate vitamin A deficiency (VAD) in the world ( Low et al., 2001

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Michael Pillay and Robert Fungo

vitamins A, C, and B 6 ( Marriott and Lancaster, 1983 ; Robinson, 1996 ), they are largely deficient of Fe, iodine, and Zn. It was observed that when cooking, banana was served as the sole weaning food for children in banana growing regions of Uganda

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Neda Keyhaninejad, Richard D. Richins, and Mary A. O’Connell

; these pigments are important for attracting pollinators and seed dispensers. In humans and other animals, specific carotenoids provide essential vitamin precursors; β-carotene and β-cryptoxanthin are pro-vitamin A forms of carotenoids ( Yeum and Russell