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Ben-Hong Wu, Ning Niu, Ji-Hu Li, and Shao-Hua Li

may be regulated by this ratio. The aim of the present study was to assess the effect of different LF treatments on protein variation in grape berry skin using two-dimensional gel electrophoresis (2-DE). Materials and Methods Plant materials and

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Ren-jun Feng, Li-li Zhang, Jing-yi Wang, Jin-mei Luo, Ming Peng, Jun-feng Qi, Yin-don Zhang, and Li-fang Lu

Corporation, Kyoto, Japan). Two-dimensional gel electrophoresis (2-DE). The 2-DE was carried out as previously described ( Zhang et al., 2012 ). Protein concentration was normalized to 1 g·L −1 in rehydration buffer [8 mol·L −1 urea, 4% (w/v) CHAPS, 10% (v

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Carole L. Bassett

Levels of the large subunit (LSU) of rubisco (ribulose 1,5 bisphosphate carboxylase/oxygenase) were measured in vegetative and floral organs of `Violet' Japanese morning glory (Ipomoea [Pharbitis] nil Roth). Identification of the LSU in polypeptides separated by two-dimensional gel electrophoresis allowed estimation of the relative abundance of this polypeptide in the organs examined. Further quantitation was achieved by immunoblotting protein extracts either alone or in combination with various amounts of extracts from other organs. The amount of LSU decreased in the order leaves > cotyledons > sepals > corollas > androecium, gynoecium. The relative abundance of LSU in sepals (74%) compared to photosynthetically competent organs [leaves (100%) and cotyledons (81%)] suggests that sepals may be photosynthetically competent in supporting development of the other floral organs.

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Stephen P. Lee, Paul M. Chen, Tony H.H. Chen, Diane M. Varga, and Eugene A. Mielke

A proportion of `d'Anjou' pear fruit (Pyrus communis L.) developed a disorder, “black speck” or “skin speckling”, after prolonged controlled atmosphere (CA) storage (1% O2, - 0.5 C). A comparative study of biochemical components revealed that there was no significant difference in succinic, citric, fumaric, and pyruvic acids between the speckled' and normal skin tissues. The content of malic acid in the affected tissue was almost three times lower than that in the normal tissue. The specific activity of NADP-malic enzyme (EC 1.1.1.40) in the affected tissue was also lower, but the total activities were similar. The affected tissue contained higher percentages of dry matter and soluble proteins than the normal tissue. Two-dimensional gel electrophoresis of proteins showed that two groups of novel polypeptides appeared only in the affected skin tissue. This study indicated that a certain proportion of `d'Anjou' pear fruit might have been exposed to unfavorable preharvest environmental stresses, and, therefore, could no longer tolerate the subsequent semi-anaerobic and chilling stresses during prolonged CA storage.

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Chenping Xu, Zhongchun Jiang, and Bingru Huang

. Protein concentration was determined according to Bradford (1976) using a commercial dye reagent (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin as a standard. Two-dimensional gel electrophoresis. An IPGPhor apparatus (GE Healthcare

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Choun-Sea Lin, Nien-Tzu Liu, De-Chih Liao, Jau-Song Yu, Chuang-Hwei Tsao, Chao-Hsiung Lin, Chih-Wen Sun, Wann-Neng Jane, Hsing Sheng Tsay, Jeremy Jian-Wei Chen, Erh-Min Lai, Na-Sheng Lin, Wei-Chin Chang, and Chung-Chih Lin

under light conditions and resolved by two-dimensional-gel electrophoresis. About 250 protein spots were detected on two-dimensional-gels within the pH range of 4 to 7 and a MW range of 17 to 76 kDa ( Fig. 3 ). Three protein spots, with pI values and MW

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Fan Cao, Xinwang Wang, Zhuangzhuang Liu, Yongrong Li, and Fangren Peng

a modified trichloroacetic acid–acetone precipitation method ( Zhou et al., 2016 ), and the two-dimensional gel electrophoresis procedure was referenced from differential protein analysis of pecan graft union formation ( Mo et al., 2017 ). The two-dimensional

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Patrick Burgess and Bingru Huang

separation were performed using the acetone/trichloroacetic acid extraction and two-dimensional gel electrophoresis method of Xu et al. (2008) . Following 21 d of drought stress, second and third fully expanded leaves were collected, immediately frozen in

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Tatsiana Espevig, Chenping Xu, Trygve S. Aamlid, Michelle DaCosta, and Bingru Huang

, H. Klose, J. Gobom, J. 2005 Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization–time of flight mass spectrometry Proteomics 5 1902 1913 Griffith, M. Antikanen, M. Hon, W

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Jinyu Wang, Bo Yuan, Yi Xu, and Bingru Huang

.A. 1998 Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis Electrophoresis 19 837 844 Oliver, D.J. Neuburger, M. Bourguignon, J. Douce, R. 1990 Glycine metabolism by plant mitochondria