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Elliot H. Norden, Paul M. Lyrene, and Jose X. Chaparro

-incompatible. The number of hybrid plants we obtained by pollinating 4301 flowers of tetraploid highbush blueberries with pollen from diploid V. elliottii (≈18 triploids and three tetraploids) was extremely low compared with the results of tetraploid × diploid

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Paul M. Lyrene

Several hundred hybrid seedlings were produced by pollinating flowers of tetraploid highbush blueberry cultivars with pollen from 13 plants of Vaccinium stamineum that were selected as tetraploids following colchicine treatment. The hybrids were intermediate between the parents in many characteristics. They were less vigorous than the parents, but 46 plants flowered when 1.3 years old from seed. The F1 hybrids produced pollen abundantly, but only 30% of the microspores appeared potentially viable when viewed at 250X. F1 flowers that were pollinated with pollen from either parent taxon or with pollen from a different F1 hybrid produced thousands of well-developed seeds. The F1 hybrids were less successful when used as male parents in backcrosses to highbush, but 4790 well-developed seeds were obtained by pollinating 3250 highbush flowers. Flowers on F1 plants had long peduncles and pedicels, giving an open raceme. The flowers were open in the bud and had anther awns, two characteristics from V. stamineum. Berries on the F1 plants had black skins, and the ripe berries of 11 F1 plants had red to purple pulp like their V. stamineum parent. Berries on the hybrids were juicy. They had little or no bitterness typical of V. stamineum, and most had a pleasing balance of sugar and acid.

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Paul M. Lyrene

s of 0.057, 0.035, and 0.023. Mean berry weight for 23 wild tetraploid highbush blueberry plants ( V. fuscatum ) from Alachua County, FL, ranged from 0.14 to 0.54 g, with an overall mean for the 23 plants of 0.25 g ( Lyrene and Sherman, 1980

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Dario J. Chavez and Paul M. Lyrene

). Obtaining fertile tetraploid hybrids by crossing V. darrowii with tetraploid highbush blueberry depends on 2n gamete production in V. darrowii , which is infrequent and confined to certain V. darrowii genotypes. Several methods have been used to

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Hirotoshi Tsuda, Hisato Kunitake, Mai Yamasaki, Haruki Komatsu, and Katsunori Yoshioka

have successfully produced several hybrids in Vaccinium ( Ballington, 1980 , 2001 ; Darrow and Camp, 1945 ; Lyrene, 2011 ; Rousi, 1963 ), but our previous attempts to cross diploid shashanbo with tetraploid highbush blueberry cultivars have not

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Paul M. Lyrene

. This study had two goals: 1) to see if hybrids could be obtained by crossing tetraploid highbush blueberry cultivars with tetraploid V. arboreum produced using colchicine and 2) to see if the hybrids would be sterile or fertile. Materials and Methods

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Dario J. Chavez and Paul M. Lyrene

of V. darrowii (2x) × V. corymbosum (4x) ranged from 2.76% to 64.04% ( Table 5 , data from individual crosses not shown). When used to pollinate tetraploid highbush blueberry, pollen from tetraploid V. darrowii resulted in higher fruit set

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Paul M. Lyrene

blueberry cross ((highbush cultivar × Vaccinium arboreum ) × highbush cultivar) HortScience 48 146 149 Lyrene, P.M. 2016 Phenotype and fertility of intersectional hybrids between tetraploid highbush blueberry and colchicine-treated Vaccinium stamineum

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Mark K. Ehlenfeldt, Allan W. Stretch, Nicholi Vorsa, and Arlen D. Draper

'Hannah's Choice' is an early-ripening, tetraploid, highbush blueberry (Vaccinium corymbosum L.) that was developed by the cooperative breeding program of the Agricultural Research Service of the U.S. Department of Agriculture (USDA) and the New Jersey Agricultural Experiment Station (NJAES). It was named because it represents an improvement in sweetness, firmness, and flavor over currently grown early cultivars.

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Patricio A. Brevis, Nahla V. Bassil, James R. Ballington, and James F. Hancock

among SHB cultivars; and 3) to estimate the impact of wide hybridization on genetic relatedness and heterozygosity of highbush blueberry. Materials and Methods Pedigree information. Pedigrees of 107 tetraploid highbush blueberry cultivars