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Kurt Knoblauch and A.E. Einert

Iris rhizomes were dug and graded by size. One hundred rhizomes, avg. wt. 23.4 g., were singly potted in 6 inch plastic standard pots. The pots were plunged, to the pot rim, into fresh sawdust, in an open coldframe.

After 4 weeks in the coldframe, and at subsequent two week intervals, pots were brought into a greenhouse for forcing. Half of each group was forced under long day conditions by night break, following a natural daylength. The other half received continuous lighting. Forcing studies were terminated when irises in the field bloomed.

Bloom dates were similar under long days and continuous light treatments. The highest percentage of plants bloomed under long days. Stalk heights at anthesis averaged 12 to 18 in. Stalk height decreased as natural cooling time increased in plants under long days, but not under continuous light. Plants with shorter flower stalks also had shorter foliage.

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Yuexin Wang, Zoran Jeknić, Richard C. Ernst and Tony H.H. Chen

A protocol was developed for efficient plant regeneration of Iris germanica L. `Skating Party' from suspension cultures. Suspension cultures were maintained in Murashige and Skoog (MS) basal medium (pH 5.9) supplemented with 290 mg·L–1 proline, 50 g·L–1 sucrose, 5.0 μm 2,4-D, and 0.5 μm Kin. Suspension-cultured cells were transferred to a shoot induction medium (MS basal medium supplemented with 10 mg·L–1 pantothenic acid, 4.5 mg·L–1 nicotinic acid, 1.9 mg·L–1 thiamine, 250 mg·L–1 casein hydrolysate, 250 mg·L–1 proline, 50 g·L–1 sucrose, 2.0 g·L–1 Phytagel, 0.5 μm NAA, and 12.5 μm Kin). Cell clusters that proliferated on this medium differentiated and developed shoots and plantlets in about 5 weeks. Regeneration apparently occurred via both somatic embryogenesis and shoot organogenesis. A series of experiments was conducted to optimize conditions during suspension culture to maximize subsequent plant regeneration. Parameters included 2,4-D and Kin concentrations, the subculture interval, and the size of cell clusters. The highest regeneration rate was achieved with cell clusters ≤280 μm in diameter, derived from suspension cultures grown for 6 weeks without subculturing in liquid medium containing 5 μm 2,4-D and 0.5 μm Kin. Up to 4000 plantlets with normal vegetative growth and morphology could be generated from 1 g of suspension-cultured cells in about 3–4 months. Chemical names used: 2,4-dichlorophenoxyacetic acid (2,4-D); kinetin (Kin); 1-naphthaleneacetic acid (NAA).

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Xiaojie Zhao, Guihong Bi, Richard L. Harkess, Jac J. Varco and Eugene K. Blythe

). Table 1. Plant height, leaf SPAD reading, and number of rhizomes (with diameter >1cm) in 2013 of container-grown ‘Immortality’ tall bearded iris fertigated with five different nitrogen (N) rates. Spring flowering of ‘Immortality’ iris occurred from late

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Xiaojie Zhao, Guihong Bi, Richard L. Harkess, Jac J. Varco, Tongyin Li and Eugene K. Blythe

Because of their showy, colorful flowers and sword-shaped leaves, TB iris ( Iris germanica L.) has potential as a specialty cut flower. Tall bearded iris plants are composed of four parts: basal sword-shaped leaves (usually called fans

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Xiaojie Zhao, Guihong Bi, Richard L. Harkess and Eugene K. Blythe

tall bearded iris. Plants were treated with different NH 4 :NO 3 ratios from Apr. to Sept. 2013. Rhizomes were planted in Aug. 2012 and data were collected weekly in 2013. ns , *Nonsignificant or significant at P ≤ 0.05, respectively. It is well

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Li Xu, Suzhen Huang, Yulin Han and Haiyan Yuan

., 2003a , 2011 ; Kim et al., 2009 ). I. germanica L., which is regarded as one of the most horticulturally important tall bearded irises, has been cultivated ever since ancient time with hundreds of commercially valuable cultivars ( Huang et al., 2003

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Zhuping Fan, Yike Gao, Ling Guo, Ying Cao, Rong Liu and Qixiang Zhang

stimulate floral initiation in tall bearded iris HortScience 45 S296 S297 Harlan, H.V. Pope, M.N. 1922 The use and value of back-crosses in small-grain breeding J. Hered. 13 319 322 Jeknic, Z. Jeknic, S. Jevremovic, S. Subotic, A. Chen, T.H.H. 2014