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Ying Qu, Xue Bai, Yajun Zhu, Rui Qi, Geng Tian, Yang Wang, Yonghua Li and Kaiming Zhang

generation of reactive oxygen species (ROS), such as hydroxyl radicals (·OH), superoxide anions (O 2 ·- ), and hydrogen peroxide. Although well known for the damage they cause to cells, ROS are considered signalling molecules in plant stress perception ( Zhou

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Chen Chen, Meng-Ke Zhang, Kang-Di Hu, Ke-Ke Sun, Yan-Hong Li, Lan-Ying Hu, Xiao-Yan Chen, Ying Yang, Feng Yang, Jun Tang, He-Ping Liu and Hua Zhang

disproportionation (a type of redox reaction) to produce molecular oxygen and H 2 O 2 , which is further catalyzed by peroxidase and catalase ( Vallino et al., 2009 ). SOD activity is usually induced along with overproduction of superoxide anions ( Benes et al., 1995

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Shuai-Ping Gao, Kang-Di Hu, Lan-Ying Hu, Yan-Hong Li, Yi Han, Hui-Li Wang, Kai Lv, Yong-Sheng Liu and Hua Zhang

three times. Determination of malondialdehyde, hydrogen peroxide, and superoxide anion. The contents of MDA, H 2 O 2 and • O 2 − were determined by the procedures described by Zhang et al. (2010a) . Fruit samples (5.00 ± 0.05 g) were ground in 3 mL

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Ting-Ting Li, Zhi-Rong Li, Kang-Di Hu, Lan-Ying Hu, Xiao-Yan Chen, Yan-Hong Li, Ying Yang, Feng Yang and Hua Zhang

prepared by using glucose and bovine serum albumin as the standard, respectively. The contents of reducing sugar and soluble protein were expressed as mg·g −1 FW. Determination of hydrogen peroxide (H 2 O 2 ), superoxide anion (·O 2 − ), and MDA. For the

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Lu Gan, Xunzhong Zhang, Silu Liu and Shuxia Yin

demonstrate that GB pretreatment could reduce cell membrane damage under drought stress and contribute to repairing the damage resulting from drought stress. Fig. 4. Effects of glycinebetaine (GB) on leaf ( A ) malondialdehyde (MDA) and ( B ) superoxide anion

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Wenting He, Weiming Guo and Zhongchun Jiang*

Effects of two pretreatments, i.e., ultrasonic wave (UW) and ultrasonic wave plus preservative solution (UW+PS), on water conditions of flower stem and membrane stability of petals in Nymphaea tetragona during 6-d cold wet storage. Compared with no pretreatment control, the two pretreatments prolonged the vase life and improved water conditions of the cut flower during cold storage to different degrees. Fresh weight of flower stems and relative water content of petals increased during cold storage. The water utilization efficiency of flower stem and water potential in different parts of flower stem were improved significantly as a result of the pretreatments. Although both pretreatments helped the cut flowers maintain favorable water relations, the effects of UW + PS combined pretreatment were better than UW pretreatment alone. In addition, UW and UW+PS inhibited the increase in the contents of lipid peroxidation product malondialdehyde (MDA) and superoxide anion in petals. UW + PS promoted superoxide dismutase (SOD) and catalase (CAT) activities in petals during cold storage to a greater degree than did UW.

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Zhengrong Hu, Erick Amombo, Margaret Mukami Gitau, Aoyue Bi, Huihui Zhu, Liang Zhang, Liang Chen and Jinmin Fu

; Nanjing Jiancheng Bioengineering Institute). For the anti O 2 .− activity determination, the electron transport material and chromogenic agent were added to react with superoxide anion free radical, which produced by the system of xanthine and xanthine

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Chang-Xia Du, Huai-Fu Fan, Shi-Rong Guo and Takafumi Tezuka

(pH 6.0; 2925 μL). The reaction was started by adding 50 μL of the enzyme solution at room temperature. One unit of LOX activity was defined as the amount of enzyme required to increase 0.1 absorbance at 234 nm·min −1 . The superoxide anion (O 2

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Sukhvinder Pal Singh and Zora Singh

Postharvest oxidative stress is a phenomenon caused by an imbalance in the production and scavenging of reactive oxygen species (ROS) in the fruit during postharvest handling and storage ( Hodges et al., 2004 ). ROS such as superoxide anion (O 2

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Zhen Shu, Yimin Shi, Hongmei Qian, Yiwei Tao and Dongqin Tang

.S. Chen, J.C. 2007 Flower senescence 256 277 Gan S.S. Senescence processes in plants Blackwell Publishing Oxford, UK ` Rich, P. Bonner, W.D. 1978 The sites of superoxide anion generation in