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Yulan Xiao and Toyoki Kozai

A photoautotrophic or sugar-free medium micropropagation system (PAM) using five large culture vessels (volume = 120 L each) with a forced ventilation unit for supplying CO2-enriched air was developed and applied to commercial production of calla lily (Zantedeschia elliottiana) and china fir (Cunninghamia lanceolata) plantlets. The culture period of calla lily plantlets in the PAM was reduced by 50%, compared with that in a conventional, photomixotrophic micropropagation system (PMM) using small vessels each containing a sugar-containing medium. Percent survival ex vitro of calla lily plantlets from the PAM was 95%, while that from the PMM was 60%. The production cost of calla lily in the PAM was reduced by about 40%, compared with that in the PMM, and the initial investment per plantlet for the PAM was ≈10% lower than that for the PMM. The sales price of ex vitro acclimatized calla lily plantlet was increased by 25% due to its higher quality, compared with plantlets produced in the PMM.

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How-Chiun Wu and Chun-Chih Lin

affecting chlorophyll content. Their results revealed that a relatively high concentration of BAP concentration (0.5 mg·L −1 ) reduced the chlorophyll content in statice plantlets cultured on sugar-free medium enriched with 1500 μmol·mol −1 CO 2 . It was

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Shanshan Seng, Jian Wu, Jiahui Liang, Fengqin Zhang, Qiuyan Yang, Junna He, and Mingfang Yi

at 22 °C in growth chamber with 16/8 h light/dark photoperiod, with light intensity of 54 μmol·m −2 ·s −1 . For abiotic stress, cormels were placed in a petri plate in sugar-free medium containing 200 m m mannitol, 200 m m glucose, 200 m m sucrose

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Zengqiang Ma, Shishang Li, Meijun Zhang, Shihao Jiang, and Yulan Xiao

Institute of Chinese Medicine Taiwan Kozai, T. 2005 Introduction 1 5 Kozai T. Afreen F. Zobayed S.M.A. Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant

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How-Chiun Wu, Mei-Ling Kuo, and Chia-Min Chen

. Afreen, and S.M.A. Zobayed (eds.). Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system. Springer, Dordrecht, The Netherlands Langford, P.J. Wainwright, H. 1987 Effects of sucrose concentration on the

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Meijun Zhang, Duanduan Zhao, Zengqiang Ma, Xuedong Li, and Yulan Xiao

production. The losses during acclimatization accounted for 20% to 50% of the plantlets ex vitro. Photoautotrophic micropropagation (PA), using a sugar-free medium and leafy explant, in which plantlets use CO 2 in the air as the sole carbon source, has

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Maren E. Veatch-Blohm and Lindsay Morningstar

salinity of wastewater treated by aquatic plants Rev. Environ. Health 23 149 166 Kozai, T. Xiao, W. Nguyen, Q.T. Afreen, F. Zobayed, S.M.A. 2005 Photoautotrophic (sugar-free medium) micropropagation systems for large scale commercialization Prop. Ornam