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Yiran Li, Akiha Abe, Takuichi Fuse, Tomonari Hirano, Yoichiro Hoshino and Hisato Kunitake

crude protein extract treatment ( Li et al., 2015 ). It has also been demonstrated that stylar crude protein extracts induced SI response in apple pollen tubes, as well as purified S-RNase protein in vitro ( Meng et al., 2014 ). In this study, we used

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Yiran Li, Asuka Uchida, Akiha Abe, Akihiro Yamamoto, Tomonari Hirano and Hisato Kunitake

pollen culture system to provide a stable environment for pollen grain growth and succeeded in simulating SI-like responses in vitro using stylar crude protein extracts ( Uchida et al., 2012 ). A high inhibition rate was observed after incompatible