percentage of ovaries that exhibit swelling ( Aloni et al., 1999 ; Polowick and Sawhney, 1985 ) and the extent of ovary swelling ( Cruz-Huerta et al., 2011 ) increase. Increased source-sink ratio also increases the proportion of swollen ovaries. Flower fresh
Rebecca L. Darnell, Nicacio Cruz-Huerta, and Jeffrey G. Williamson
Guohai Xia, Lailiang Cheng, Alan Lakso, and Martin Goffinet
exemplifies the central role of the source-sink relationship in determining apple fruit growth, which also provides a very useful framework for understanding the effect of many other factors on apple fruit growth and final fruit size. Increasing nitrogen (N
Rui Wang, Yuqing Gui, Tiejun Zhao, Masahisa Ishii, Masatake Eguchi, Hui Xu, Tianlai Li, and Yasunaga Iwasaki
photosynthesis and nutrients, or high sink activity to compete for more photoassimilates, which were mainly found in fruits such as oilseed rape, grape, tomato, and cucumber ( Li et al., 2015 ; Marion et al., 2014 ). In fact, the mechanism of source–sink
Ted M. DeJong, Romeo Favreau, Mitch Allen, and Przemyslaw Prusinkiewicz
Modeling source–sink interactions and carbohydrate partitioning in plants requires a detailed model of plant architectural development, in which growth and function of each organ is modeled individually and carbohydrate transport among organs is modeled dynamically. L-PEACH is an L-system-based graphical simulation model that combines supply/demand concepts of carbon partitioning with an L-system model of tree architecture to create a distributed supply/demand system of carbon allocation within a growing tree. The whole plant is modeled as a branching network of sources and sinks, connected by conductive elements. An analogy to an electric network is used to calculate the flow and partitioning of carbohydrates between the individual components. The model can simulate multiple years of tree growth and be used to demonstrate effects of irrigation, crop load, and pruning on architectural development, tree growth, and carbon partitioning. Qualitative model outputs are viewed graphically as the tree “grows” on the computer screen while quantitative output data can be evaluated individually for each organ or collectively for an organ type using the MatLab software.
Rui Zhou and Bruno Quebedeaux
Photosynthesis and carbohydrate metabolism in apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] source leaves were monitored during a 7-day period after source-sink manipulations by girdling or partial defoliation treatments. In the girdling treatment, sorbitol, sucrose, glucose, and starch accumulated in leaves, and net photosynthetic rates (Pn) at 350 μL·L-1 CO2 decreased during a 7-day period. Pn measured at 1000 μL·L-1 [CO2] was also decreased but the changes were less. Stomatal conductance and intracellular CO2 concentration decreased markedly in leaves of girdled shoots. When shoots were partially defoliated, starch and glucose concentrations in remaining source leaves declined steadily during the 7-day study period. Sorbitol and sucrose concentrations decreased during the first 2 days after defoliation, then increased the following 5 days. Pn of the remaining leaves measured at ambient and elevated CO2 levels were enhanced markedly. Aldose-6-phosphate reductase activity in source leaves increased markedly from 27.5 to 39.2 μmol·h-1·g-1 fresh weight (FW) after partial defoliation but remained unchanged in leaves after girdling. Selective and maximum sucrose phosphate synthase (SPS) activities increased following partial defoliation and decreased following girdling. ADP-glucose pyrophosphorylase activity remained relatively unchanged in the partial defoliation treatments but increased markedly in the girdled-shoot leaves. These results suggested that girdling-induced photosynthetic inhibition is mainly due to stomatal limitation, however, the photosynthesis enhancement by partial defoliation may be due primarily to acceleration of photosynthetic capacity per se. These studies showed that the metabolism of sorbitol, sucrose and starch, three photosynthetic end products in mature apple leaves, was coordinately regulated in source leaves in response to source-sink manipulations.
Desmond R. Layne and J.A. Flore
The source-sink ratio of l-year-old, potted `Montmorency' sour cherry (Prunus cerasus) trees was manipulated by partial defoliation (D) or continuous lighting (CL) to investigate the phenomenon of end-product inhibition of photosynthesis. Within 24 hours of D, net CO2 assimilation rate (A) of the most recently expanded source leaves of D plants was significantly higher than nondefoliated (control) plants throughout the diurnal photoperiod. Between 2 and 7 days after D, A was 30% to 50% higher and stomatal conductance rate (g,) was 50% to 100% higher than in controls. Estimated carboxylation efficiency(k) and ribulose-1,5-bisphosphate (RuBP) regeneration rate increased significantly within 2 days and remained consistently higher for up to 9 days after D. Leaf starch concentration and dark respiration rate decreased but sorbitol and sucrose concentration increased after D. The diurnal decline in A in the afternoon after D may have been due to feedback inhibition from accumulation of soluble carbohydrates (sucrose and sorbitol) in the cytosol. This diurnal decline indicated that trees were sink limited. By 9 days after D, photochemical efficiency was significantly higher than in control plants. In the long term, leaf senescence was delayed as indicated by higher A and gs in combination with higher chlorophyll content up to 32 days after D. CL resulted in a significant reduction of A, gs, k, variable chlorophyll fluorescence (Fv), photochemical efficiency, and estimated RuBP regeneration rate of the most recently expanded source leaves within 1 day. During the exposure to CL, A was reduced 2- to 3-fold and k was reduced up to 4-fold. The normal linear relationship between A and gs was uncoupled under CL indicating that A was not primarily limited by gs and since internal CO2 concentration was not significantly affected, the physical limitation to A imposed by the stomata was negligible. The decrease in Fv and photochemical efficiency indicated that leaves were photoinhibited within 1 day. The decrease in instantaneous chlorophyll fluorescence after at least 1 day of CL indicated that there was a reversible regulatory mechanism whereby the damage to photosystem II reaction centers was repaired. Leaf chlorophyll content was not altered by 1,2, or 3 days of exposure to CL, indicating that photooxidation of chlorophytl did not occur. The time to full photosynthetic recovery from CL increased as the duration of exposure increased. CL plants that were photoinhibited accumulated significant starch in the chloroplast in a companion study (Layne and Flore, 1993) and it is possible that an orthophosphate limitation in the chloroplast stroma was occurring. D plants that were continuously illuminated were not photosynthetically inhibited. After 7 days of CL, plants that were then partially defoliated yet remained in CL photosynthetically recovered within 5 days to pre-CL values. Under the conditions of this investigation, end-product inhibition of A occurred in young, potted sour cherry trees but the mechanism of action in D plants was different than in CL plants.
Eliezer E. Goldschmidt
. Synthetic growth regulators were exploited in every possible way to solve practical problems. The emergence of the source-sink concept (≈1980) partially replaced the hormonal hypothesis. The source-sink hypothesis claims that carbohydrate levels control the
Jingwei Dai and Robert E. Paull
The inflorescence of Protea neriifolia B. Br. was two-thirds of the total cut floral stem fresh weight and significantly influenced blackening of the attached 20 to 30 leaves. Floral stems harvested at five developmental stages were characterized for inflorescence diameter, fresh and dry weights, respiration, and nectar production. Inflorescence diameter and fresh and dry weights increased from stage 1 (very tight bud) to stage 5 (bracts reflexed). Respiration rate was high in stages 1 and 3. Nectar production began at stage 4 (open, cylindrical flower) and increased from 2.7 to 9.8 ml per flower with 15% to 23.5% total soluble solids as the flower opened. Postharvest inflorescence diameter, respiration rate, and nectar production increased and leaf blackening decreased when floral stems were placed in 5% (w/v) sucrose solution. Application of 14C-sucrose to a leaf subtending the inflorescence lead to >50% of the radioactivity being found in the nectar within 24 hours. These data indicate that leaf blackening in protea is the result of depletion of carbohydrate by the inflorescence, and that this depletion is primarily due to the sugar demand for nectar production.
G. Fernandez and M. Pritts
Seasonal changes in growth, photosynthetic rates, temperature, and light response curves of `Titan' red raspberry (Rubus idaeus L.) were obtained from potted plants grown under field conditions. Primocane dry weight accumulation underwent two phases of linear growth at the beginning and the end of the season, but growth slowed during fruiting. This slower rate of dry weight accumulation also coincided with an increase in root dry weight. Primocane NAR and SLA were highest early in the season. Light response curves differed depending on cane type and time of year. Floricane photosynthetic rates (A) were high during the fruiting period, while primocane A rates remained steady throughout the season. Both primocane and floricane leaflets displayed a midday drop in A rate, with a partial recovery in late afternoon. Photosynthetic rates of both primocane and floricane leaves were very sensitive to high temperatures. Temporal convergence of sink demand from fruit, primocanes, and roots occurs when plants experience high temperatures. This may account for low realized yields in raspberry and the high level of yield component compensation typical of source-limited plants.
Justine E. Vanden Heuvel*
Fruiting and vegetative greenhouse-grown cranberry uprights (Vaccinium macrocarpon Ait.) were subjected to four defoliation levels (0%, 25%, 50%, 75%) on one of three dates during the growing season. Seven days following defoliation, vines were destructively harvested and carbohydrate concentration was quantified using HPLC. Prior to new growth, defoliation did not affect the concentration of total non-structural carbohydrates (TNSC) in the uprights, or the partitioning of water-soluble (i.e., sucrose, glucose, fructose) to ethanol-insoluble (i.e., starch) carbohydrates, even though uprights with lower leaf areas had higher net CO2 assimilation rates (A). At 2 weeks post-bloom, TNSC concentration was reduced in defoliated vines, although A was not affected by defoliation. Prior to harvest, TNSC concentration was reduced in vines subjected to defoliation while A was unaffected, although the positive relationship between soluble carbohydrate concentration and leaf area per upright reached an asymptote, while the direct relationship between starch concentration and leaf area remained linear. Carbohydrate production and partitioning of an upright was unaffected by the presence of a single fruit throughout the experiment. These results suggest that carbohydrate production in cranberry uprights may be sink-limited prior to fruiting, and then becomes source-limited as the growing season progresses.