highest yield of pigment and polyphenols (colorants) in Ya-nang with 15.5, marigold ( Tagetes erecta L.) with 8.4, and ebony tree with 7.7. Overall, results of the solvent extraction can be explained by the relationship between the polarity of solvents
Panthip Boonsong, Natta Laohakunjit, Orapin Kerdchoechuen and Frank B. Matta
Denys J. Charles and James E. Simon
Essential oils were extracted from leaves, flowers, and stems of Ocimum basilicurn, O. kilimandscharicum, and O. micranthum by solvent extraction, hydrodistillation, and steam distillation for essential oil content and the oil analyzed by GC and GC/MS for composition. While the yield of essential oil was consistently higher from steam distillation than hydrodistillation, a similar number of compounds was recovered from both hydrodistillation and steam distillation. Though the relative concentration of the major constituents was similar by both methods, the absolute amounts were higher with steam distillation. Essential oil content and composition varied by plant species and plant part. Essential oil content was highest in flowers for O. basilicum and in leaves for O. micranthum. No significant differences were observed in essential oil yield and relative concentration of major constituents using fresh or dry samples and using samples from 75 g to 10 g of dry plant tissue. While minor differences between hydrodistillation and steam distillation were observed, both methods resulted in high yields and good recovery of essential oil constituents. Hydrodistillation is a more-rapid and simpler technique than steam and permits the extraction of essential oil where steam is not accessible.
T. R. Hamilton-Kemp, J. H. Loughrin and R. A. Andersen
Two methods for collecting headspace vapors produced by plant samples are presented. The first involves entraining volatiles in a stream of air and trapping the entrained compounds on a porous polymer such as Tenax. The volatiles are recovered from the trap by solvent extraction or heat desorption and analysed by gas chromatography. A second method entails removing headspace vapor above plant material with a gas-tight syringe and injecting the sample directly into the gas chromatograph. An evaluation of the usefulness of these techniques will be presented.
Liangli Yu, Denys J. Charles, Amots Hetzroni and James E. Simon
The volatiles of muskmelon (Cucumis melo L. reticulatis cv. Mission) were sampled by dichloromethane extraction and dynamic headspace methods and analyzed by gas chromatography (GC) and GC–mass spectroscopy (MS). A total of 34 constituents were identified, with esters contributing 8%–92% of the total volatiles. Butyl propionate, ethyl 3-methylpentanoate, hexadecanoic acid, methyl (methylthio)acetate, propyl butyrate, phenylpropyl alcohol, and vanillin, were recovered only by solvent extraction, while hexanal was only detected using dynamic headspace sampling. Methyl butyrate 35.2%, ethyl acetate 17.1%, butyl acetate 11.6%, ethyl propionate 8.3%, and 3-methylbutyl acetate 6.3% were the major constituents by solvent extraction sampling method. Butyl acetate 35.5%, 3-methylbutyl acetate 20.9%, ethyl acetate 7.3%, 2-butyl acetate 5.6%, and hexyl acetate 3.8% were the major constituents recovered by headspace sampling. Fruit tissue was also separated into five layers (exocarp, outer mesocarp, middle mesocarp, inner mesocarp, and seed cavity) and the volatile constituents differed significantly in content and composition by tissue.
Niels O. Maness, Jamal Bizri, Michael W. Smith, C. Zhang and Gerald H. Brusewitz
Partial oil extraction is being investigated as a means to increase oxidative stability and provide reduced fat pecan halves. Supercritical extraction with carbon dioxide provided a means to extract twenty to thirty percent of resident oil, with little to no kernel damage and leaving no harmful residues in the kernel or the extracted oil. Variances in extraction time, temperature, pressure and total carbon dioxide volume used for extraction with a continuous flow extractor will be discussed. Fatty acid composition of oils extracted using supercritical carbon dioxide was essentially the same as oils obtained by solvent extraction and by cold press. Fatty acid yield in the oils was greater for supercritical extraction compared to the other two methods. Oxidative stability for extracted and unextracted pecans, determined using an accelerated aging technique, will be compared. Supported by USDA grant 92-34150-7190 and the Oklahoma Agricultural Experiment Station.
Niels O. Maness, Michael W. Smith, C. Zhang and Gerald H. Brusewitz
Techniques to reduce the oil content of shelled pecans using supercritical CO2 have been developed, and the effect of partial oil extraction on kernel quality is being investigated. Extraction conditions induce little kernel damage and allow for up to 30% oil reduction. Extraction temperature, at 40 or 80C, influenced kernel color. Regardless of temperature, extracted nut meat was lighter in color. Testa color increased in redness for kernels extracted at 80C compared to kernels extracted at 40C. Extracted oil was amber. Fatty acid composition of oil obtained with supercritical CO2 was essentially the same as oil obtained by organic solvent extraction and by cold press. Investigations to determine the effect of oil reduction on pecan shelf life are described. This research was supported by U.S. Department of Agriculture grant 92-34150-7190, Oklahoma Center for Advancement of Science and Technology grant AR4-044, and the Oklahoma Agricultural Experiment Station.
Bhaskar Bondada, Peter D. Petracek and Jim Syvertsen
Recent interest in reducing nitrate levels in ground water has stimulated the re-examination of foliar application of urea on citrus trees. Because the cuticle is the primary barrier to foliar uptake, we examined the diffusion of 14C-urea through isolated citrus leaf cuticles. Cuticles were enzymatically isolated from leaves of the four youngest nodes (1 month to 1 year old) of pesticide-free grapefruit trees. The diffusion system consisted of a cuticle mounted on a receiver cell containing stirred buffer solution. Urea (1 μL) was pipetted onto the cuticular surface, and buffer solution was sampled periodically through the side portal of the receiver cell. The time course of urea diffusion was characterized by lag (time to initial penetration), quasi-linear (maximum penetration rate), and plateau (total penetration) phases. Apparent drying time was less than 30 min. Average lag time was about 10 min. The maximum penetration rate occurred about 40 min after droplet application and was about 2% of the amount applied per hour. Rewetting stimulated further penetration. The total penetration averaged about 35% and tended to decrease with leaf age. Dewaxing the second node cuticles by solvent extraction significantly increased maximum penetration rates (30% of the amount applied per hour) and total penetration (64%).
Jane E. Lancaster, Julie Farrant and Martin L. Shaw
Three onion (Allium cepa L.) cultivars, `Southport White Globe', `Grano', and `Pukekohe Longkeeper' were grown at low to high S (at 0.5, 1.8, 3.0 or 4.0 meq·L-1) in hydroponic culture. Differential solvent extractions of bulbs were used to isolate quantitatively cell contents, cell wall proteins, and cell wall residue. The weight of the cell fractions, their S content, and the S content of intact bulbs were determined. Bulb characteristics of fresh weight (FW), firmness, soluble solids concentration (SSC), and soluble sugars were also determined. For all three cultivars, bulb FW increased with S from 0.5 to 4.0 meq·L-1. Sulfur had a significant effect on bulb firmness. Onion bulbs grown with S at 0.5 meq·L-1, the lowest S concentration, were significantly softer than onion bulbs grown at the highest concentration of 4.0 meq·L-1. Varying the S supply had a major effect on dry weight (DW) allocation to the cell wall residue. Bulbs of all three cultivars grown at the lowest S had significantly less DW in the cell walls compared to S at 3.0 or 4.0 meq·L-1. In contrast to the effect of S supply on DW allocation, varying S supply had no effect on total bulb S, free SO4 -2, and on the S content of the cell contents and the cell wall residue and only a minor effect on cell wall proteins. There was no significant effect of S supply on either SSC or soluble sugars. At low S nutrition, which is limiting to the growth of onion bulbs, cell wall deposition is reduced, with a consequent decrease in bulb firmness. The S composition of the cellular components is maintained at the expense of bulb growth.
Weiguang Yi and Hazel Y. Wetzstein
40 °C dried tissues were similar and significantly higher that fresh and 70 °C dried samples. The highest TEAC value (TE 382.5 μM·g −1 ) was observed with sun-dried tissues followed by 40 °C, 70 °C, and fresh samples. The effect of solvent extraction
L. Carolina Medina, Jerry B. Sartain and Thomas A. Obreza
accelerating their natural release mechanism in a laboratory setting. Various increasingly aggressive solvent extraction procedures performed on an unground 30-g sample were used to characterize N release properties of SRF products. Each extraction was designed