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Zuguo Cai, Wenfang Zeng, Liang Niu, Zhenhua Lu, Guochao Cui, Yunqin Zhu, Lei Pan, Yifeng Ding, and Zhiqiang Wang

. Simple sequence repeat (SSR) marker name and linkage group of the 78 SSRs used for peach related species identification. z PCR amplification. PCR amplification was performed in 20-µL reaction volumes containing 20 ng of genomic DNA, 10 µL of 2× Taq PCR

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Xiaoli Wang, Zhiyong Wang, Li Liao, Xinyi Zhang, and Changjun Bai

carpetgrass accessions from China ( Table 1 ). Table 2. Amplification information of simple sequence repeat primers in carpetgrass accessions. Primer screening and DNA amplification. Fourteen primer pairs with clear gel bands, reproducible fragments, and

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Margaret Pooler and Hongmei Ma

ultimately to the field. Table 1. Cross, parents, and simple sequence repeat (SSR) profiles of Prunus parents and hybrids tested in this study. z DNA extraction, simple sequence repeat primers, and polymerase chain reactions. Total genomic DNA was extracted

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Qijing Zhang and Dajun Gu

subgenus Lithocerasus , and 22 accessions (seven species and two hybrids) to subgenus Cerasus ( Table 1 ). Table 1. Forty Prunus accessions used in genetic relationship analysis among 10 rootstock species from China by simple sequence repeat. Genomic

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Phillip A. Wadl, Xinwang Wang, John K. Moulton, Stan C. Hokanson, John A. Skinner, Timothy A. Rinehart, Sandra M. Reed, Vincent R. Pantalone, and Robert N. Trigiano

Simple sequence repeat (SSR), also called microsatellites, are sections of DNA consisting of tandemly repeated mono-, di-, tri, tetra-, or pentanucleotide units that occur in abundance within the genomes of most eukaryotes ( Powell et al., 1996

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Qing Shen, Hua Bian, Hai-yan Wei, Li Liao, Zhi-yong Wang, Xiao-yan Luo, Xi-peng Ding, Zhenbang Chen, and Paul Raymer

additional four cultivars from the University of Georgia ( Table 1 ). Table 1. The origins of 58 Paspalum vaginatum accessions and four cultivars used in genetic diversity analysis revealed with simple sequence repeat markers. All materials were propagated

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Benard Yada, Phinehas Tukamuhabwa, Bramwell Wanjala, Dong-Jin Kim, Robert A. Skilton, Agnes Alajo, and Robert O.M. Mwanga

polymorphic loci (SAMPL) ( Tseng et al., 2002 ), and simple sequence repeat markers (SSRs) ( Gichuru et al., 2006 ). Microsatellites developed for genotyping sweetpotato ( Buteler et al., 1999 ; Hu et al., 2004 ) have been widely used for diversity analysis

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Kadir Uğurtan Yılmaz, Sezai Ercişli, Bayram Murat Asma, Yıldız Doğan, and Salih Kafkas

marker system to use for a particular application depends on its ease of use and the particular objectives of the investigation ( Rafalski et al., 1996 ). Recently, inter-simple sequence repeat (ISSR) markers have emerged as an alternative system with the

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Eiichi Inoue, Lin Ning, Hiromichi Hara, Shuan Ruan, and Hiroyuki Anzai

; Morimoto et al., 1997 ), inter-simple sequence repeat markers ( Casasoli et al., 2001 ), restriction fragment length polymorphism markers ( Morimoto et al., 1997 ), and amplified fragment length polymorphism markers ( Yamamoto et al., 1998 ), have been used

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Troy D. Carson, Donald B. White, and Alan G. Smith

one-, two-, or three-base tag attached to the 3′ or 5′ end of the repeat. The tag assures that the primer anneals to the end of the simple sequence repeat and thus provides greater specificity. The benefit of using ISSR PCR over other methods include