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Margaret Pooler and Hongmei Ma

ultimately to the field. Table 1. Cross, parents, and simple sequence repeat (SSR) profiles of Prunus parents and hybrids tested in this study. z DNA extraction, simple sequence repeat primers, and polymerase chain reactions. Total genomic DNA was extracted

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Eiichi Inoue, Lin Ning, Hiromichi Hara, Shuan Ruan and Hiroyuki Anzai

; Morimoto et al., 1997 ), inter-simple sequence repeat markers ( Casasoli et al., 2001 ), restriction fragment length polymorphism markers ( Morimoto et al., 1997 ), and amplified fragment length polymorphism markers ( Yamamoto et al., 1998 ), have been used

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Daofeng Liu, Jing Ma, Jianfeng Yang, Tien V. Nguyen, Huamin Liu, Renwei Huang, Shunzhao Sui and Mingyang Li

dicotyledenous plants such as Arabidopsis ( Lawson and Zhang, 2006 ) and Cucurbita pepo ( Blanca et al., 2011 ). Thus, AG and AAG motifs may be common features of SSRs in dicotyledenous plants. Table 1. Summary of simple sequence repeat types in the

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John McCallum, Susan Thomson, Meeghan Pither-Joyce, Fernand Kenel, Andrew Clarke and Michael J. Havey

large genome size typical of Allium species ( Bennett and Leitch, 1995 ). Fischer and Bachmann (2000) reported development of a set of genomic dinucleotide simple sequence repeat (SSR) markers from onion. As a result of complex amplification

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P. Boccacci, A. Akkak, D. Torello Marinoni, G. Bounous and R. Botta

Microsatellite or simple sequence repeat (SSR) markers show many characteristics of the ideal molecular marker, and recent studies have shown that loci developed in one species may allow analysis in taxonomically related species. In this study, 52 primer pairs developed in two oak species—Quercus robur L. and Quercus petraea (Matt.) Lieb.—were used to amplify DNA of 5 chestnut cultivars; 28 of them yielded amplicons and 12 polymorphic loci were selected and used to fingerprint 12 european chestnut (Castanea sativa Mill.) cultivars grown in the Piedmont region of northwestern Italy. The number of alleles per locus ranged from 3 to 8, mean expected heterozygosity was 0.592 (range: 0.288 to 0.868), and mean observed heterozygosity was 0.667 (range: 0.333 to 1.000). The results demonstrate the usefulness of some SSR markers isolated in Quercus for the fingerprinting and genetic mapping of Castanea cultivars.

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Zuguo Cai, Wenfang Zeng, Liang Niu, Zhenhua Lu, Guochao Cui, Yunqin Zhu, Lei Pan, Yifeng Ding and Zhiqiang Wang

. Simple sequence repeat (SSR) marker name and linkage group of the 78 SSRs used for peach related species identification. z PCR amplification. PCR amplification was performed in 20-µL reaction volumes containing 20 ng of genomic DNA, 10 µL of 2× Taq PCR

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Xiaoli Wang, Zhiyong Wang, Li Liao, Xinyi Zhang and Changjun Bai

carpetgrass accessions from China ( Table 1 ). Table 2. Amplification information of simple sequence repeat primers in carpetgrass accessions. Primer screening and DNA amplification. Fourteen primer pairs with clear gel bands, reproducible fragments, and

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Qijing Zhang and Dajun Gu

subgenus Lithocerasus , and 22 accessions (seven species and two hybrids) to subgenus Cerasus ( Table 1 ). Table 1. Forty Prunus accessions used in genetic relationship analysis among 10 rootstock species from China by simple sequence repeat. Genomic

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Phillip A. Wadl, Xinwang Wang, John K. Moulton, Stan C. Hokanson, John A. Skinner, Timothy A. Rinehart, Sandra M. Reed, Vincent R. Pantalone and Robert N. Trigiano

Simple sequence repeat (SSR), also called microsatellites, are sections of DNA consisting of tandemly repeated mono-, di-, tri, tetra-, or pentanucleotide units that occur in abundance within the genomes of most eukaryotes ( Powell et al., 1996

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Benard Yada, Phinehas Tukamuhabwa, Bramwell Wanjala, Dong-Jin Kim, Robert A. Skilton, Agnes Alajo and Robert O.M. Mwanga

polymorphic loci (SAMPL) ( Tseng et al., 2002 ), and simple sequence repeat markers (SSRs) ( Gichuru et al., 2006 ). Microsatellites developed for genotyping sweetpotato ( Buteler et al., 1999 ; Hu et al., 2004 ) have been widely used for diversity analysis