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Takahiro Tezuka, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, and Masayuki Oda

). This method enables in vivo adventitious shoot regeneration from stumps after decapitation of the primary shoot and all lateral branches. Harada et al. (2005) reported that 79 shoots were regenerated from the cut surface of primary shoots and lateral

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Xiaojuan Zong, Brandon J. Denler, Gharbia H. Danial, Yongjian Chang, and Guo-qing Song

incorporate target genes of interest for its further improvement. To achieve this, an efficient in vitro shoot regeneration system is needed. Successful in vitro shoot regeneration from seed-derived or leaf explants has been reported for several Prunus

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Fatemeh Haddadi, Maheran Abd Aziz, Hossein Kamaladini, and Seyed Ali Ravanfar

shoot regeneration in cultivated strawberry was demonstrated using several types of in vitro cultured explants. However, the highest regeneration success was obtained from leaf explants for most of the cultivars analyzed ( Passey et al., 2003

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Cary J. Hebert, Darren H. Touchell, Thomas G. Ranney, and Anthony V. LeBude

sp. in subsection Edgeworthia or subsection Maddenia . In vitro shoot regeneration from leaves of Rhododendron sp. is most commonly stimulated by a combination of the cytokinins, 6-(γ,γ-dimethylallylamino) purine (2iP) or zeatin, and auxins

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L. Xu, G.F. Liu, and M.Z. Bao

essential. We had adopted the regeneration method described by Brand and Lineberger (1988) for shoot regeneration from leaves of Formosan sweetgum using woody plant medium (WPM; Lloyd and McCown, 1980 ) containing 11.1 μ m 6-benzyladenine (BA) and 0.54 μ

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Carrie A. Radcliffe, James M. Affolter, and Hazel Y. Wetzstein

). Similar protocols have been developed for economically important members of the Ericaceae, including blueberry, cranberry, lingonberry, and rhododendron in which TDZ was used alone or in combination with 2iP or IAA to promote shoot regeneration ( Cao and

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Elisabeth M. Meyer, Darren H. Touchell, and Thomas G. Ranney

shown 2,4-D to be ineffective for in vitro shoot regeneration ( Touchell et al., 2008 ). Efficient in vitro regeneration systems provide a tool to manipulate ploidy and improve ornamental features. Polyploidy in some plants may result in desirable

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Samir C. Debnath and Danny L. Barney

; Qu et al., 2000 ). Additionally, a shoot regeneration system can be used to identify and/or induce somaclonal variants and to develop transgenic plants following genetic transformation of plant cells. To produce superior Vaccinium clones more

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Mahmoud B. Arif and Houchang Khatamian

Surface sterilized stem nodal sections of western soapberry (Sapindus drummondii Hook. & Arn.) were cultured on Murashige and Skoog (MS) medium. The basal media consisted of one half and full strength MS medium each supplemented with the following (mg-1): Nicotinic acid 0.5, pyridoxine Hcl 0.5, Glycine 2.0, myo-inositol 100, sucrose 30,000 and agar 8000. Each medium also was supplemented with either 0, 0.01, 0.1, 1.0 and 10 mg/l Thidiazuron (TZD) or 0, 0.5, 2.0 and 5 mg/l 6-Benzyladenine (BA). The pH of all media was adjusted to 5.8 ± 0.1. The culture media were autoclaved at 120°C at 1.5 Kgcm-1 pressure for 15 min.

The highest percentage of nodal sections resulting in shoot regeneration occurred on 1/2 MS with TZD at 0.01 mg/l and MS medium containing 0.5 mg/l of BA Increasing the TZD concentration above 0.1 mg/l resulted in callus formation on cut surfaces.

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Mohsen Hesami and Mohammad Hosein Daneshvar

improvement of genus Ficus via transgenic engineering ( Sharma et al., 2015 ). According to the study that is about shoot regeneration via indirect organogenesis through callus phase conducted by Jaiswal and Narayan (1985) , the maximum 50% regeneration