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Jose Martínez-Calvo, Gerardo Llácer, and Marisa Luisa Badenes

dwarf ilarvirus , Prunus necrotic ringspot ilarvirus , Apple chlorotic leafspot closterovirus , and Apple mosaic ilarvirus . Literature Cited Egea, J. Burgos, L. Martínez-Gómez, P. Dicenta, F. 1999 Apricot breeding for sharka resistance at CEBAS

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Jose Martínez-Calvo, Gerardo Llácer, and Maria Luisa Badenes

. Dicenta, F. 1999 Apricot breeding for sharka resistance at CEBAS-CESIC, Murcia (Spain) Acta Hort. 121 207 210 Kölber, M. 2001 Workshop on Plum pox Acta Hort. 550 249 255 Llácer, G. Cambra, M. 1998 Thirteen years of sharka disease in Valencia, Spain Acta

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José Egea, Manuel Rubio, José A. Campoy, Federico Dicenta, Encarna Ortega, María D. Nortes, Pedro Martínez-Gómez, Antonio Molina, Antonio Molina Jr, and David Ruiz

breeding for sharka resistance at CEBAS-CSIC, Murcia (Spain) Acta Hort. 488 153 157 Egea, J. Dicenta, F. Burgos, L. 2004 ‘Rojo Pasión’ apricot HortScience 39 1490 1491 Egea, J. Ruiz, D. Dicenta, F. Burgos, L. 2005 ‘Murciana’ apricot HortScience 40 254 255

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Maria L. Badenes, Jose Martínez-Calvo, Helena Gómez, and Elena Zuriaga

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Jean-Michel Hily, Michel Ravelonandro, Vern Damsteegt, Carole Bassett, Cesar Petri, Zongrang Liu, and Ralph Scorza

Constructs with self-complementary sequences separated by an intron produce “hairpin” RNA [intron-hairpin-RNA (ihpRNA)] structures that efficiently elicit posttranscriptional gene silencing (PTGS). In the current study, the authors use this technology to confer resistance to plum pox virus (PPV) in herbaceous and woody perennial plants by silencing the PPV–coat protein (CP) gene. The authors confirmed the high capacity of ihpRNA constructs for inducing RNA silencing in Nicotiana benthamiana Domin., as more than 75% of the transformants displayed PTGS as evaluated by specific small interfering RNA (siRNA) production. The authors demonstrated that ihpRNA constructs provided PPV resistance, and they found a correlation between the length of the PPV sequence introduced in the ihpRNA constructs and the frequency of transgenic-resistant plants. Plants transformed with the full-length sequence produced a higher percentage of resistant lines. The authors further demonstrated for the first time that ihpRNA technology is applicable to a woody perennial species. A transgenic plum (Prunus domestica L.) PPV-CP ihpRNA line showed gene silencing characteristics (hypermethylation of the transgene sequence and specific siRNA production) and resistance to PPV infection 16 months after inoculation.

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J. Egea, D. Ruiz, F. Dicenta, and L. Burgos

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J. Egea, F. Dicenta, and L. Burgos

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J. Egea, P. Martínez-Gómez, F. Dicenta, and L. Burgos

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David Ruiz, Manuel Rubio, Pedro Martínez-Gómez, Jesús López-Alcolea, Federico Dicenta, Encarna Ortega, María Dolores Nortes, Antonio Molina, Antonio Molina Jr., and Jose Egea

apricot cultivars HortScience 28 148 150 Egea, J. Burgos, L. Martínez-Gómez, P. Dicenta, F. 1999 Apricot breeding for sharka resistance at CEBAS-CSIC, Murcia (Spain) Acta Hort. 488 153 157 Egea, J. Dicenta, F. Burgos, L. 2004 ‘Rojo Pasión’ apricot

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José Egea, Jose A. Campoy, Federico Dicenta, Lorenzo Burgos, Jose L. Patiño, and David Ruiz

Egea, J. Burgos, L. Martínez-Gómez, P. Dicenta, F. 1999 Apricot breeding for sharka resistance at CEBAS-CSIC, Murcia (Spain) Acta Hort. 488 153 157 Hough, L.F. Bailey, C.H. 1982 30 years of apricot breeding in New Jersey Acta Hort. 121 207 210 Kölber, M