information for precision irrigation and N management. Water deficit has an adverse effect on transpiration, which has been confirmed in sap flow studies for many woody tree species such as olive [ Olea europaea ( Fernández et al., 2001 ; Tognetti et al
Rangjian Qiu, Taisheng Du, Shaozhong Kang, Renqiang Chen and Laosheng Wu
Ismail A. Hussein and Marshall J. McFarland
Two-year-old, greenhouse-grown, potted `Granny Smith' apple (Malus domestica Borkh.) trees grafted on seedling, M.7 EMLA, or Mark rootstock were subjected to water stress by withholding irrigation for three successive days. Sap flow rates were measured with steady-state, heat-balance stem gauges; daily water use was measured with an electronic balance; and instantaneous transpiration was measured with a diffusion porometer. Differences in the sap flow among the three rootstocks were observed by the third day of stress treatment. The cumulative sap flow over the 3-day stress period was higher for trees on seedling and M.7 EMLA rootstocks than for those on Mark. Cumulative sap flow was reduced on the second and third days of water stress compared to sap flow in the control. The diurnal peak of sap flow rate was flattened on the second and third days of stress compared to the pattern for the control and first day of stress treatment. In general, the trees on the standard rootstock (seedling) were least affected by the water stress; trees on the full dwarf rootstock (Mark) were the most affected. Good agreement between cumulative sap flow measurements and gravimetric measurements indicates that the steady-state, heat-balance method is practical and accurate.
Mark A. Rose
New electronic biosensors that directly monitor plant physiological and morphological processes are now being developed for use in research and commercial applications. Although methods for measuring sap flow by applying heat to stems have been used for more than 20 years, they have usually been intrusive, required empirical calibrations and conversions, and been too fragile for rugged commercial environments. A more-promising method for monitoring sap flow is balancing the thermal fluxes into and out of a stem segment using heat sources wrapped around a stem. Constant heat-balance sap-flow gauges have been used for the direct, accurate, non-invasive, and continuous measurement of sap flow rate in many herbaceous and woody plants, including forest and fruit trees, vines, landscaping shrubs, and numerous agronomic plants. The performance of sap-flow gauges has steadily improved as they have been used in a wider range of basic and applied research. Research is now being conducted to use these gauges as on-line sensors to schedule irrigations, monitor plant stress, and even control the greenhouse environment.
Patricia R. Knight, J. Roger Harris and Jody K. Fanelli
Root severance during field harvesting alters the water status of a tree, resulting in water stress and reduced post-transplant growth. Two experiments, using Acer rubrum L. (red maple), determined the influence of root severance at harvest on sap flow and xylem embolism. Trees 1.5–1.8 m tall (4 years old) were utilized in the first experiment, and trees 1.2–1.5 m tall (2 years old) were utilized in the second. Sap flow sensors were installed on the 4-year-old trees prior to root severance and remained on the trees until 1 week after harvest. Within 1 day after root severance sap flow was reduced and remained lower than nontransplanted (control) trees for the remainder of the experiment. Leaf stomatal conductance (Cs) of transplanted trees 1 week after root severance was lower than that of control trees, but leaf water potentials (ψ) were similar. In the second experiment, sap flow was reduced relative to control trees within 2 h after root severance. Although Cs was reduced 4 hours after root severance, ψ was not. Embolism increased within 24 hours of root severance. These results indicate that root severance quickly induces increased levels of embolism, which is associated with reduced sap flow.
Kenneth A. Shackel, R. Scott Johnson, Charles K. Medawar and Claude J. Phene
The heat balance method was used to estimate transpirational sap flow through 60- to 75-mm-diameter stems (trunks) of 3-year-old peach [Prunus persica (L.) Batsch. cv. O'Henry] trees under field conditions. On rare occasions, heat balance estimates agreed well with independent lysimetric measurements, but on most occasions, heat balance estimates of sap flow were unrealistic in both direction and magnitude. In some cases, the errors in sap flow approached two orders of magnitude and were always the result of a calculation involving division by a very small and sometimes negative temperature differential between the stem surface temperature above and below the gauge heater. The occurrence of negative temperature differentials under positive transpiration conditions may be inconsistent with a fundamental assumption in the heat balance model, namely that temperature differentials are solely a consequence of the dissipation of energy supplied to the gauge heater. In the absence of heating power applied to the gauge, temperature differentials exceeding - 1C were correlated with the rate of change in stem temperature, indicating that ambient conditions themselves can impose a bias in gauge signals and, hence, influence gauge accuracy. Our results suggest that the effect of ambient conditions on gauge signals should be critically evaluated before considering heat balance estimates of sap flow as reliable under any given conditions.
J.M. Tarara and J.C. Ferguson
Management strategies like “deficit irrigation” in wine grapes require accurate, reliable information on vine water use, making direct measurements of vine transpiration highly desirable. The heat-balance sap flow method has the advantages of being non-invasive and requiring no other calibration beyond a zero-flow set. Potential violations of the method's assumptions were dealt with and the heat balance method successfully applied to mature grape vines under conditions of extremely high sap flow. Greenhouse studies suggested that vines transpire at night, up to 9.5% of the total 24-h water loss, thus violating the zero-flow assumption for setting the gauge constant. Using a predetermined gauge constant caused smaller errors than using daily, pre-dawn constants set in situ. The steady-state assumption was violated only in early and late hours of the day, and the inclusion of a term to account for the change in heat stored by the stem only marginally improved daily estimates of water use. The assumption of radially uniform temperature across the heated stem segment is violated at very high flows (e.g., >700 g·h–1), but can be corrected for by using wider heaters and adjusting the placement of thermocouples. For a mature, potted vine in the greenhouse, the maximum absolute error in cumulative daytime water use between a sap gauge and a precision load cell was about –10%, with the gauge almost exclusively underestimating water loss. A custom-built, 20-gauge system was run continuously in the field for 90 days. Vine-to-vine variability in water use was not accounted for by normalizing sap flow by leaf area, suggesting that it is critical to include in any field study the largest number of gauges that are technically feasible.
Said A. Hamido, Kelly T. Morgan and Davie M. Kadyampakeni
objective was to determine how HLB may affect water relations of citrus, in particular water use, sap flow, and stem water potential. Materials and methods Experimental setup and greenhouse measurements. The experiment was conducted from 7 Jan. 2014 to 31
Said A. Hamido, Kelly T. Morgan, Robert C. Ebel and Davie M. Kadyampakeni
measurements at selected commercial sites. Sap flow. Sap flow measurements were conducted four times between Feb. 2014 and Aug. 2015 including dry and wet seasons ( Table 1 ) as measured by the heat balance method using an automated Flow32-1K flow system
Kristof Vermeulen, Kathy Steppe, Katrien Janssen, Peter Bleyaert, Jan Dekock, Jean-Marie Aerts, Daniel Berckmans and Raoul Lemeur
segment for which the sap-flow rate is determined. This so-called “heat-balance” technique has already been examined for potential use in a wide range of crops, including cotton ( Gossypium hirsutum ) ( Dugas, 1990 ), cucumber ( Cucumis sativus ) ( Kitano
Bruce W. Wood
sap during the late winter to early spring transition period before and during budbreak likely influence floral initiation and evocation. Positive pressure within a tree’s above-ground stem structure leads to late winter xylem sap flow. This pressure