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Xiuli Shen, William S. Castle, and Frederick G. Gmitter Jr

obtained on Murashige and Skoog (MS) medium ( Murashige and Skoog, 1962 ) supplemented with 6-benzylaminopurine (BA) at 0 to 35.6 μM and root induction on MS medium supplemented with indole-3-butyric acid (IBA) from 4.3 to 17.4 μM ( Shen et al., 2009b

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Chao Dong, Xue Li, Yue Xi, and Zong-Ming Cheng

. The number of proliferated microshoots was counted after 4 weeks. Rooting induction. Microshoots, equal to or longer than 1.5 cm in length, were excised from cultures and used to study root induction. The treatments were one-fourth MS basal medium

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James F. Harbage, Dennis P. Stimart, and Carol Auer

The influence of root initiation medium pH on root formation was investigated in relation to uptake and metabolism of applied IBA in microcuttings of Malus ×domestica Borkh. `Gala' and `Triple Red Delicious'. Root formation and uptake of H 3-IBA were related inversely to root initiation medium pH. Maximum root count (10.3 roots) and IBA uptake were observed at pH 4.0. Regardless of pH, overall root count of `Gala' was higher (13.5 roots) than `Triple Red Delicious' (4 roots). Uptake of IBA was highest at pH 4.0 for `Gala' (1.7% uptake) and at pH 4 and 5 for `Triple Red Delicious' (0.75% uptake). Metabolism of IBA was the same regardless of root initiation medium pH or cultivar examined. One-half of the IBA taken up was converted to a compound that coeluted with IBAsp during high-performance liquid chromatography. Apparently, pH regulates root formation by affecting IBA uptake but not metabolism. The level of auxin in tissue appeared unrelated to root formation between genotypes. Chemical names used: 1H-indole-3-butyric acid (IBA); 5-H 3-indole-3-butyric acid (H 3-IBA); indole-3-butrylaspartic acid (IBAsp).

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Ling Yu, Hongwei Chen, Peipei Hong, Hongli Wang, and Kefeng Liu

illumination of 300 μmol·m −2 ·s −1 at 25 ± 2 °C, 60% RH. The medium contained 1/4 MS medium that was supplemented with various concentrations of NAA (0, 2.23, 4.46, 11.15, or 22.29 µM). Root induction frequency was recorded and calculated: Root induction

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Mohamed S. Elmongy, Xiuyun Wang, Hong Zhou, and Yiping Xia

( Elmongy et al., 2018a , 2018b ). HA-mediated root induction has been studied in several plants under in vitro and in vivo conditions ( Mora et al., 2012 ). Remarkable improvements were shown in various morphological, physiological, and biochemical aspects

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Alvine Ornella Tchouga, Vincent Deblauwe, Stephanie Astride Mouafi Djabou, Giovanni Forgione, Rachid Hanna, and Nicolas Niemenak

) vitamins and 3.0% sucrose. For shoot growth, media were solidified with 0.3% (w/v) gelrite (Duchefa Biochemie B.V., Haarlem, The Netherlands), whereas for root induction 0.7% Bacto agar (Liofilchem s.r.l., Roseto degli Abruzzi, Italy) was used. In each

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Xiaoxin Shi, Lili Yang, Guijun Yan, and Guoqiang Du

d after subculture. Effect of medium pH on adventitious root induction. New shoots longer than 1.5 cm were inoculated onto the rooting medium containing ½-strength MS, indoleacetic acid 1.0 mg·L −1 , IBA 0.4 mg·L −1 , sucrose 25.0 g·L −1 , and agar 6

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Jalil Dejampour, Islam Majidi, Solmaz Khosravi, Sevil Farhadi, and Atena Shadmehr

for aeration. The coverings were removed completely after 1 month. Every 2 weeks, the plantlets were fertilized with 0.1% (w/v) N:P:K solution (1:1:1). Table 2. Rooting induction treatments including various indole butyric acid (IBA) concentrations for

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Ningguang Dong, Qingmin Wang, Junpei Zhang, and Dong Pei

biological characteristics of adventitious roots after application of exogenous auxin. Although these studies have provided important information concerning the action of this hormone, the mechanism of auxin in adventitious root induction has not been clearly

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Zhenghui Tang, Honghui Lin, Lei Shi, and Weilun Chen

leaf and basal leaf explants, followed by successful greenhouse establishment of regenerated plants, and discusses the conditions required for efficient shoot elongation and root induction. Materials and Methods Plant material was provided by the