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John Clemens and R. Hugh Morton

Containerized plants of Heliconia psittacorum L.f. × H. spathocircinata Aristeguieta `Golden Torch' were grown in a greenhouse for 8 months from early summer to winter under selected combinations of N, P, and K. Fertilizer rates ranged from zero to rates that exceeded those reported in the literature by 50% to 100%. Biomass variables (vegetative and inflorescence dry weight, and leaf area) were predicted to be maximized at high N and high N to P, and N to K ratios corresponding to N-P-K application rates of 1.2, 0.5, and 0.6 kg·m-3, respectively (≈2:1:1). However, the number of shoots and flowers produced per rhizome were maximal at lower N to K ratios (1:1). Flower yield could therefore be optimized with appropriate fertilization, provided attention was paid to the N to K ratio so that the size of plants and their flowers was not compromised by efforts to increase shoot and flower number. The heavier the rhizome planted, the shorter the time for shoot emergence and flowering to occur, and the greater the number of flowers harvested. However, rhizome weight had no effect on number of shoots to emerge. The probability of shoots flowering declined markedly with order of shoot emergence, although this could be increased with appropriate mineral nutrition. The maximum number of leaves subtending the inflorescence (seven) was obtained at high N and P rates. Flower production was probably limited by declining solar radiation in autumn, and by within-plant competition for rooting space.

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Jacob George, Harsh Pal Bais, G.A. Ravishankar and P. Manilal

Response surface methodology was utilized in statistical optimization of three quality factors (the number of multiple shoots, shoot length, and number of leaves) pertaining to regeneration of plantlets from leaf calli of Decalepis hamiltonii Wight. & Arn. (swallow root). The variables evaluated were the levels of sucrose, BA, and NAA each at two different concentrations. Response surfaces for shoot length and multiple shoot number were useful in achieving optimal levels of media constituents and in understanding their interactions, but response surfaces for number of leaves were not. The data indicate that sucrose, BA, and NAA levels may be manipulated to increase or decrease quality factors chosen. This approach may be useful in developing a micropropagation protocol for D. hamiltonii. Chemical names used: benzyladenine (BA); napthaleneacetic acid (NAA).

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Rizwan Maqbool, David Percival, Qamar Zaman, Tess Astatkie, Sina Adl and Deborah Buszard

the second objective was to recommend fertilizer rates that optimize these same factors. We chose to use response surface methodology and canonical analysis as aids in modeling and examining the relationships between fertilizer rates and plant

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Le Luo, Yichi Zhang, Yingnan Wang, Tangren Cheng, Huitang Pan, Jia Wang and Qixiang Zhang

surface methodology for laccase production by Peniophora sp. hpF04 J. Microbiol. Biotechnol. 2 46 56 Sun, Y.L. Mu, C.H. Chen, Y. Kong, X.P. Xu, Y.C. Zheng, H.X. Zhang, H. Wang, Q.C. Xue, Y.F. Li, Z.X. 2016 Comparative transcript profiling of maize

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Maedza K. Vuwani, Mpumelelo Nkomo, Wonder Ngezimana, Nokwanda P. Makunga and Fhatuwani N. Mudau

. 2013 Optimisation of kaffir lime leaves ( Citrus hystrix ) volatile oil extraction by pressurised liquid extraction (PLE) using response surface methodology (RSM). Universiti Teknologi MARA, PhD Diss Haukioja, E. Ossipov, V. Koricheva, J. Honkanen, T

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Isaac Zipori, Arnon Dag, Yizhar Tugendhaft and Reuven Birger

Acta Hort. 824 337 348 Segovia-Bravo, K.A. Garcia-Garcia, P. Lopez-Lopez, A. Garrido-Fernandez, A. 2012 Effect of inert atmosphere on postharvest browning of Manzanilla olives and optimization by response surface methodology of the aqueous treatments J

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Jiaqi Yan, Megan M. Dewdney, Pamela D. Roberts and Mark A. Ritenour

371 378 Nevarez, L. Vasseur, V. Debaets, S. Barbier, G. 2010 Use of response surface methodology to optimize environmental stress conditions on Penicillium glabrum , a food spoilage mould Fungal Biol. 114 490 497 Paul, I. van Jaarsveld, A.S. Korsten

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Anna Marín, Anne Plotto, Lorena Atarés and Amparo Chiralt

as biocontrol agents in plants Biocontrol Sci. Technol. 26 1451 1470 Ghafoor, K. Park, J. Choi, Y. 2010 Optimization of supercritical fluid extraction of bioactive compounds from grape ( Vitis labrusca B.) peel by using response surface methodology

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Wendy A. Johnson, Raymond A. Cloyd, James R. Nechols, Kimberly A. Williams, Nathan O. Nelson, Dorith Rotenberg and Megan M. Kennelly

response surface methodology Ecology 74 442 454 Dallal, G.E. 2007 The little handbook of statistical practice. USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA. Apr. 2011. < http://www.jerrydallal.com/LHSP/LHSP.HTM > Dudt, J

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Yan Li, Hongyan Qi, Yazhong Jin, Xiaobin Tian, Linlin Sui and Yan Qiu

lipase-catalyzed hexyl acetate in n-hexane by response surface methodology J. Agr. Food Chem. 49 1203 1207 Song, J. Bangerth, F. 2003 Fatty acids as precursors for aroma volatile biosynthesis in pre-climacteric and climacteric apple fruit Postharvest Biol