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Jingwei Dai and Robert E. Paull

The inflorescence of Protea neriifolia B. Br. was two-thirds of the total cut floral stem fresh weight and significantly influenced blackening of the attached 20 to 30 leaves. Floral stems harvested at five developmental stages were characterized for inflorescence diameter, fresh and dry weights, respiration, and nectar production. Inflorescence diameter and fresh and dry weights increased from stage 1 (very tight bud) to stage 5 (bracts reflexed). Respiration rate was high in stages 1 and 3. Nectar production began at stage 4 (open, cylindrical flower) and increased from 2.7 to 9.8 ml per flower with 15% to 23.5% total soluble solids as the flower opened. Postharvest inflorescence diameter, respiration rate, and nectar production increased and leaf blackening decreased when floral stems were placed in 5% (w/v) sucrose solution. Application of 14C-sucrose to a leaf subtending the inflorescence lead to >50% of the radioactivity being found in the nectar within 24 hours. These data indicate that leaf blackening in protea is the result of depletion of carbohydrate by the inflorescence, and that this depletion is primarily due to the sugar demand for nectar production.

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M. Ahmedullah

The extent of translocation of 14C-labeled photosynthates from the senescent leaf to the parent vine before leaf abscission and the short-term effects of premature leaf removal on the carbohydrate balance of the vine were studied by using autoradiography and trapping 14CO2 respired from the treated leaf. The treated leaf abscissed 1.5 days after administering the label. The plant was harvested after natural leaf abscission. The radioactivity recovered from the plant, excluding the treated leaf, was 20% of the input. Radioactivity was detected in the roots, trunk, shoot, and leaves. Most of the radioactivity remained in the trunk and the young and old roots. The implications of premature leaf removal by mechanical harvesting on the carbohydrate balance of the vine are discussed.

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Karine Fouldrin, Anis Limami, and Thierry Lamaze

During forcing, the witloof chicory taproot produces an etiolated bud, the chicon. The axis of this organ often is brown as a consequence of a disorder associated with a localized Ca deficiency. The effect of the main anions (NO3 -, Cl-, SO4 2) in the nutrient solution on Ca (45Ca) absorption and translocation in the chicon was investigated. Although the amount of Ca that accumulated in the chicon was not affected by nutrient solution composition, Ca (45Ca) mobility was modified. The amount of radioactivity in the chicon increased slightly when the main anion in the solution was sulfate and decreased markedly when the main anion was chloride, compared to nitrate. Calculations of the specific radioactivity of Ca reaching the chicon and in root tissue suggest that, when slowed down, Ca transport consists of a homogeneous flow in all root tissues, whereas, in other cases, Ca moves along a preferential pathway, such as the xylem vessels. Modifying the anionic composition of the nutrient solution to avoid a localized Ca deficiency is discussed.

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Margrethe Serek and Arne Skytt Andersen

We thank Henrik Saaby Johansen for radioactivity-measurement assistance, Michael S. Reid for critical review, Peggy Hale and Carol Adams for data analysis advice, and Ove Nielsen for the rose plants. The experiments were supported by a grant

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J.M. Goatley Jr., A.J. Powell Jr., M. Barrett, and W.W. Witt

Laboratory studies were conducted to determine the basis for chlorsulfuron selectivity between Kentucky bluegrass (Poa pratensis L. cv. Kenblue) and tall fescue (Festuca arundinacea Schreb. cv. Rebel). Tall fescue absorbed and translocated more foliar-applied [14C]-labeled chlorsulfuron from the treated leaf than Kentucky bluegrass. The two species absorbed similar amounts of chlorsulfuron from nutrient solution into the roots, but tall fescue translocated more of the absorbed radioactivity to the shoots. Tall fescue metabolized chlorsulfuron in the shoots slightly more slowly than Kentucky bluegrass. Allof these factors apparently contributed to the higher tolerance of Kentucky bluegrass than of tall fescue to chlorsulfuron. Chemical name used: (2-chloro-N-[[4-methoxy-6-methyl-1,3,5 -triazin-2-yl)amino]-carbonyl] benzenesulfonamide) (chlorsulfuron).

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Hanling Yu and Claude Willemot

We examined the relationship between reduced galactolipid content in tomato fruit at 4C and chilling injury. Galactolipid biosynthesis from 14C-acetate was compared in pericarp discs of cold-tolerant `New York 280' (`NY') and -sensitive `Early Cherry' (`EC') at 4C and 20C. Labeled lipids were separated by 2D-TLC. Labeled monogalactosyldiglyceride (MGDG) molecular species were hydrolyzed using a position-specific lipase; the fatty acids released were hydrogenated and separated according to chain length by reverse-phase TLC. At 4C, the relative amount of radioactivity was reduced in MGDG and enhanced in phosphatidylcholine (PC) in both cultivars, in comparison with labeling at 20C. In discs from fruit chilled for 6 h, labeling was similar in `NY' and `EC'. In fruit held at 4C for 8 days, labeling of MGDG was reduced and that in PC was enhanced to a greater extent in chilling-sensitive `EC' than in `NY'. The proportion of the MGDG label in eukaryotic species (i.e., the ratio in C18/C16 fatty acids in position sn-2), was less in `EC' at 4C than at 20C, even for fruit held at 4C for only 6 h. The ratio was little affected in `NY'. The data indicate that biosynthesis of eukaryotic MGDG was inhibited in tomato fruit at chilling injury-inducing temperatures.

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Justine E. Vanden Heuvel, Evangelos D. Leonardos, John T.A. Proctor, K. Helen Fisher, and J. Alan Sullivan

Potted `Chardonnay' grapevines (Vitis vinifera L.) with either two or three shoots were grown in a greenhouse for one month and then transferred to a phytotron room, where either one or two shoots were shaded. Twenty-four days after transfer, leaves at the fifth node of either the light-adapted or shade-adapted shoot were exposed to a 2-hour pulse of 14CO2. Both light environment and number of shade shoots on the vine had a significant effect on photosynthate partitioning within the plant following a 22-hour chase. Leaves fed with 14CO2 on a light-adapted shoot translocated 26.1% and 12.7% more radioactivity to the roots and trunk, respectively, than leaves from shade-adapted shoots. Photosynthates were exported from light-adapted leaves to shade-adapted shoots (1.3% of total 14C in plant). The number of shaded shoots and the light environment of the fed leaf had a large effect on partitioning of photosynthates among ethanol-insoluble, water-soluble, and chloroform-soluble fractions within the leaf. Recovered 14C in the water-soluble fraction of the fed leaf appeared to be affected more by number of shoots than by light environment of the fed leaf. The data suggest that there is a sink effect on initial carbon partitioning patterns in grapevine leaves. Sink strength may have a greater role than light environment. A large proportion of interior leaves versus exterior leaves may be costly with respect to the carbohydrate budget of a vine.

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Juan Valenzuela-Valenzuela and Norman K. Lownds

Resistance to the auxin-like herbicide picloram has been reported in a yellow starthistle population growing in Washington. In addition, this population is cross resistant to clopyralid, another auxin-like herbicide. To understand the mechanism of resistance to clopyralid, studies were was conducted to determine uptake and translocation and to characterize clopyralid-induced ethylene production in the susceptible (S) and resistant (R) biotypes. R and S yellow starthistle plants were grown under ambient greenhouse conditions until full rosette stage and then transferred to a growth chamber (14-hour photoperiod, 25°C, 200 mol·m–2·s–1) 48 hours before treatment. Radiolabel solutions were prepared from 12C and 14C-clopyralid. Each treatment in the uptake experiment contained 0.009 Ci (20,000 dpm) and in the translocation experiment 0.225 Ci (500,000 dpm). Clopyralid 11.7 mm (420 g a.e./ha) solutions were applied as six 0.5-L droplets to the adaxial surface of completely expanded leaves using a microsyringe. Radioactivity was quantified by Liquid Scintillation Spectrometry. Uptake was determined at specified times after treatment. Almost all clopyralid uptake occurred within the first 2 hours, with no significant differences between the two biotypes. The amount of picloram translocated was 2.4%, 40.2%, and 50.7% of that absorbed at 2, 24, and 96 hours after treatment, respectively, but was not different between biotypes. Clopyralid induced about ten times greater ethylene production in S than in R. Ethylene production was followed by epinasty and chlorosis but appears to play only a small role in the resistance mechanism.

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James O. Denney and George C. Martin

Fruit removal force (FRF) and percent leaf drop (LD) of fruit-bearing olive (Olea europaea L.) shoots were examined 120 hours after being sprayed with ethephon at 600 mg·liter-1 and held under controlled-environmental conditions analogous to those found in the field in California at harvest time in mid-October. FRF was not significantly affected by solution pH, but FRF of all treated shoots was significantly lower than that of the untreated controls. Only at pH 5 was percent LD significantly greater than that of the controls, but, of the shoots treated with ethephon, the lowest percent LD occurred at pH 3. Percent LD after treatment with ethephon at pH 3 was not affected by application time, but FRF was significantly less than the controls' when shoots were treated at 7 am or 12 pm but not at 5 pm or 10 pm. Adding NAA to the ethephon solution raised FRF and adding BA lowered FRF compared to ethephon alone. Adding NAA or BA did not mitigate percent LD significantly. Adding BA advanced anthocyanin production in fruit. Ethephon penetration of rachides was ≈70% that of petioles. Correlation between ethephon penetration of petioles and percent LD was greater than that between penetration of rachides and FRF. Correlation was significant for both tissues only in the 12 pm pH 3 treatment; correlation was also significant for petiole penetration and percent LD at pH 5. Autoradiographic studies of the 14C-ethephon penetration showed no pH effect, greater penetration into petioles than rachides, and that radioactivity was limited largely to intercellular spaces, with accumulation in vascular bundles, especially xylem. Regardless of treatment, FRF and percent LD are negatively correlated (r 2 = 0.615). Mean results to be expected using ethephon as an olive harvest aid under these conditions are an FRF of ≈3 N and a percent LD of ≈15%. The desired low FRF and percent LD were obtained by applying ethephon alone at pH 3 at 7 am. Raising ethephon solution pH does not increase harvest effectiveness. Chemical names used: (2-chloroethyl)phosphonic acid (ethephon), naphthalene acetic acid (NAA), 6-benzylaminopurine (BA).

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James S. Busse, Senay Ozgen, and Jiwan P. Palta

were cut into three equal segments to analyze the radioactivity present in top, middle, and bottom parts of the shoot. Statistical analysis. Data were analyzed using a model for a completely randomized design in PROC GLM procedure of SAS