from these different provenances have shown varying tolerances to salt, drought, and pH extremes, which can be useful when selecting genotypes for planting in different geographical regions ( Denny, 2007 ). Cercosporidium blight caused by the pathogen
Garry Vernon McDonald, Geoffrey C. Denny, Michael A. Arnold, Donita L. Bryan, and Larry Barnes
Ulrik Bräuner Nielsen and Gary A. Chastagner
Kathy Riley and Gerner Frederiksen during harvest and data collection is gratefully acknowledged. Special thanks to Langesø Estate for hosting the provenance trial and for allowing harvesting of branch samples.
Bruce W. Wood, Larry. J. Grauke, and Jerry A. Payne
An assessment of vegetative traits of pecan [Carya illinoinensis (Wangenh.) K. Koch] from a range-wide provenance collection indicated the existence of at least two distinct populations within the native range (i.e., provenances north of Texas vs. provenances in Texas and Mexico). Southern most provenances generally broke bud earlier, retained foliage later in the fall, grew larger in height and trunk diameter, had narrower leaflet droop angles, had greater leaflet tilt angles, wider limb angles, greater Zn deficiency, less black pecan aphid susceptibility, and less red coloration to foliage than did northern most provenances. Trees originating from Jaumaua, in northern Mexico, were especially noteworthy insomuch that they were by far the tallest, possessed the largest trunk diameters, the longest foliation period, and lowest Zn deficiency ratings of all provenances. One family within this Jaumaua population also exhibited a high level of cold hardiness. Family heritability (hf 2) estimates were ≥0.48 for trunk cross sectional area, date of budbreak, leaf redness, cold injury, leaflet droop angle, and leaflet tilt angle, and ≤0.39 for late season leaf fall, black pecan aphid susceptibility, zinc deficiency, and branch angle.
Ling Ma, Xingquan Rao, and Xiaoyang Chen
this study, we examined the growth rates and leaf phenotypes of 57 plant species with several different provenances grown hydroponically for more than 10 weeks. We hypothesized the following: 1) length of survival under waterlogging stress should be a
Emad Bsoul, Rolston St. Hilaire, and Dawn M. VanLeeuwen
strategy has revealed that in beech ( Fagus sylvatica L.) plants selected over 11 provenances, drought had a lesser impact on the greenhouse performance of ecotypes selected from relatively dry habitats compared with plants chosen from mesic provenances
Bryan J. Peterson and William R. Graves
wide range of soil pH or that ecotypic adaptation to localized soil pH has occurred ( Dawson et al., 2007 ; Rajakaruna, 2004 ; Snaydon, 1970 ). If the latter is true, genotypes of D. palustris from disparate provenances may respond differently to
Laura G. Jull, Thomas G. Ranney, and Frank A. Blazich
Seedlings of six provenances of Atlantic white cedar [Chamaecyparis thyoides (L.) B.S.P.] (Escambia Co., Ala., Santa Rosa Co., Fla., Wayne Co., N.C., Burlington Co., N.J., New London Co., Conn., and Barnstable Co., Mass.) were grown in controlled-environment chambers for 12 weeks under 16-hour photoperiods with 16-hour days/8-hour nights of 22/18 °C, 26/22 °C, 30/26 °C, 34/30 °C or 38/34 °C. Considerable variation in height, foliage color, and overall plant size was observed among plants from the various provenances. Seedlings from the two most northern provenances (Massachusetts and Connecticut) were most heat sensitive as indicated by decreasing growth rates at temperature regimes >22/18 °C. In contrast, plants from New Jersey and the three southern provenances (North Carolina, Florida, and Alabama) exhibited greater heat tolerance as indicated by steady or increasing growth rates and greater top and root dry weights as temperature regimes increased above 22/18 °C. Growth rates of seedlings from the four aforementioned provenances decreased rapidly at temperature regimes >30/26 °C suggesting low species tolerance to high temperatures. There were no significant differences in seedling dry matter production among provenances when temperature regimes were ≥34/30 °C. Net shoot photosynthesis and dark respiration of plants did not vary by provenance; however, net photosynthesis was temperature sensitive and decreased at temperature regimes >26/22 °C. Foliar respiration rates increased as temperature increased from 22/18 °C to 26/22 °C, but then remained relatively constant or decreased at higher temperature regimes. Plants at temperatures ≥34/30 °C exhibited severe stunting, chlorosis, and necrosis on branch tips. However, tissue concentrations of N, P, K, Ca, Mg, Fe, Zn, Cu, and Mn generally increased with temperature regimes >30/26 °C indicating that mineral nutrient concentration was not a limiting factor at high temperatures.
Larry J. Shoemake, Michael A. Arnold, and Fred T. Davies Jr.
A series of six experiments was conducted over eight years to investigate impacts of provenance on transplant establishment in landscapes and the role of adventitious root regeneration in differential genotypic responses during establishment of Platanus occidentalis L. Fall, spring, and summer transplants of container-grown half-sib families (HSF = seedlings derived from a single mother tree with unknown male parentage), including two selections native to Brazos County, Texas (Brazos-C, Brazos-D), one native to Cookeville, Tenn. (Cookeville), two Kentucky/Tennessee HSF from the Westvaco Corp. (WV-10, WV-14), and two Texas HSF from the Texas Forest Service tree improvement program (TFS-09, TFS-24), were established to determine field/landscape growth responses. Subsequent studies were conducted to investigate differential leaf gas exchange responses of TFS-09 and Cookeville during moderate water deficits and to determine root regeneration potential (RRP) responses of TFS-09, Brazos-C, WV-14, and Cookeville HSF following fall, spring, and summer transplant. To investigate consistency of within-family genotypic responses and to determine relationships among adventitious root initiation from shoot cuttings, RRP, and landscape establishment, five seedlings of TFS-09 and five from Cookeville HSF were clonally propagated and ramets tested under field and RRP conditions similar to those with seedling-derived plants. Regionally native HSF consistently grew taller, had larger trunk diameters, and often had greater survival during the first 3 years in the landscape than HSF not native to the region in which the studies were conducted. Rapidity of root regeneration among HFS at the time of transplant was the best root growth related predictor of successful landscape establishment. Some growth advantages were found using genetically improved HSF, but not as consistent an improvement as with the use of seedlings from regional provenances. Within-family variation in landscape performance was greater with nonregional Cookeville clones than with regional TFS-09 clones, however there was overlap among the more vigorous Cookeville clones and the least vigorous TFS-09 clones. Increased rapidity of root regeneration and drought adaptations related to leaf morphology and gas exchange characteristics may be involved in enhanced growth responses of Texas regional genotypes. No consistent relationships were found among adventitious rooting responses from shoot cuttings and subsequent RRP of the same genotypes from root tissues or their growth during the first 3 years in landscapes.
Geoffrey C. Denny, Michael A. Arnold, and Wayne A. Mackay
(natural) selection by a distinct ecological condition.” It is the entire basis for provenance studies ( Arnold, 2002 ). Zobel and Talbert (1984) define a provenance as “the original geographic area from which seed or other propagules were obtained” and
Laura G. Jull and Frank A. Blazich
Cones of six provenances (Escambia Co., Ala., Santa Rosa Co., Fla., Wayne Co., N.C., Burlington Co., N.J., New London Co., Conn., and Barnstable Co., Mass.) of Atlantic white cedar [Chamaecyparis thyoides (L.) B. S. P.], were collected Fall 1994 (Alabama, North Carolina, New Jersey, and Connecticut), Winter 1995 (Massachusetts), or Fall 1995 (Florida). Cones were dried for 2 months, followed by seed extraction and storage at 4°C. Seeds were then graded and stratified (moist-prechilled) for 0, 30, 60, or 90 days. Following stratification, seeds were placed at 25°C or an 8/16-hr thermoperiod of 30°/20°C with daily photoperiods of 0, 1, or 24 hr. Germination was recorded every 3 days for 30 days. Temperature, stratification, and light had significant effects on germination. However, responses to these factors varied according to provenance. Averaged over all treatments, the Alabama provenance exhibited the greatest germination (61%), followed by the Florida provenance (45%), with the remaining provenances ranging from 20% to 38%. However, there were specific treatments for each provenance that resulted in germination > 50%. The three southern provenances (Alabama, Florida, and North Carolina) required 30 days of stratification for maximum germination. They did not exhibit an obligate light requirement, but photoperiods ≥ 1 hr increased germination greatly over seeds in darkness. In contrast, the northern provenances (New Jersey, Connecticut, and Massachusetts) had an obligate light requirement. These provenances only required 30 days stratification with continuous light for maximum germination. When subjected to a 1-hr photoperiod, seeds from the northern provenances required longer durations of stratification for maximum germination. Regardless of the length of stratification, the New Jersey provenance required a 24-hr photoperiod to maximize germination. When averaged over all treatments, total germination for each provenance was greater at 30°/20°C than 25°C (43% vs. 31%).