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Zhi-Rong Li, Kang-Di Hu, Fen-Qin Zhang, Shi-Ping Li, Lan-Ying Hu, Yan-Hong Li, Song-Hua Wang, and Hua Zhang

– 2.69A 645 ) V/W; Chl b (mg·g −1 ) = (22.9A 645 – 4.68A 663 ) V/W. Each analysis was repeated three times and the results were expressed as mg·g −1 fresh weight. Determination of endoprotease and Rubisco. Protease activity was assayed according to

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Ji-Lian Zheng, Lan-Ying Hu, Kang-Di Hu, Jun Wu, Feng Yang, and Hua Zhang

assays of APX, CAT, GR, LOX, PAL, POD, PPO, SOD, and protease in apple slices. APX (EC 1.11.1.11), CAT (EC 1.11.1.6), POD (EC 1.11.1.7), and SOD (EC 1.15.1.1) were assayed according to the method by Garcìa-Limones et al. (2002) . Apple tissues (5.00 ± 0

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Mahalaxmi Veerasamy, Yali He, and Bingru Huang

, protein loss is accelerated as a result of increased protease activity and this, in turn, leads to accelerated leaf senescence ( Al-Khatib and Paulsen, 1984 ; Ueda et al., 2000 ; Zavaleta-Mancera et al., 1999 ). Plant adaptation to high temperatures also

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Paul H. Jennings and Ann Fitzpatrick

Heat shock induction of chilling tolerance in cucumber seedlings is not blocked by inhibitors of protein synthesis. Treatment of germinating seeds with cycloheximide and actinomycin-D, prior to heat shock and chilling, does not block the heat shock induction of chilling tolerance, while the inhibitors alone promote chilling tolerance of seedling roots. To test whether the heat shock effect might be acting on proteases, two protease inhibitors (bestatin and PMSF) were tested for their ability to induce chilling tolerance. Although PMSF slowed germination, it still provided protection against chilling, but bestatin was much more effective.

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Yali He, Xiaozhong Liu, and Bingru Huang

Various physiological processes may deteriorate in response to increasing temperatures, contributing to the decline in turf quality for cool-season turfgrasses during heat stress. This study was performed to investigate metabolic changes (membrane lipid peroxidation, total protein content, amino acid content, and protease activity) associated with turf quality decline for creeping bentgrass (Agrostis stolonifera Huds.) in response to gradually increasing temperatures for a short duration and prolonged exposure to lethally high temperature. Plants were subjected to increasing temperatures of 20, 25, 30, 35, and 40 °C for 7 days at each level of temperature [gradual heat stress (GHS)] or exposed to high temperature of 40 °C for 28 days [prolonged heat stress (PHS)] in growth chambers. During the GHS treatment, significant decline in turf quality occurred when plants were exposed to 30 °C for 7 days; simultaneously, malondialdehyde (MDA) content increased and total protein content in shoots decreased significantly compared to those at 20 °C. Protease activity increased at 25 °C and then decreased as temperature was elevated from 30 to 40 °C during the GHS treatment. Amino acid content decreased under GHS, beginning at 25 °C. Under the PHS treatment, turf quality declined and MDA content increased significantly, beginning at 14 days of PHS, while total protein content decreased at 7 days of PHS. Protease activity and amino acid content increased at 7 days of PHS, and then declined with longer stress duration. Our results indicated that protease activity, and amino acid and total protein content were more responsive to GHS or PHS than that of lipid peroxidation and turf quality. Changes in metabolic parameters of protease activity, amino acid and total protein content, and lipid peroxidation may contribute to leaf senescence and poor turf performance under severe or prolonged heat stress conditions for creeping bentgrass.

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Lusheng Zeng, Jiayang Liu, Robert N. Carrow, Paul L. Raymer, and Qingguo Huang

treatment for 3 d followed by drying at 40 °C for 36 h, only the high dosage of chitinase, laccase, pectinase, and protease was effective in reducing WDPT less than 60 s for the very strongly hydrophobic LDS Soil 7. For the strongly hydrophobic fairy ring

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Laura J. Chapin, Youyoun Moon, and Michelle L. Jones

plant caspase-like enzymes led to the identification of a structurally related family of cysteine proteases called the metacaspases, which are also present in protozoans and fungi ( Uren et al., 2000 ). Metacaspases have been shown to play a critical

Open access

Li Liu, Lin Jin, and Qiaosheng Guo

represented as an unweighted pair group method with arithmetic mean clustering tree. Measurements of soil enzyme activities. The activities of soil sucrase, urease, protease, neutral phosphatase, catalase, and polyphenol oxidase were measured by colorimetric

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Yiran Li, Akiha Abe, Takuichi Fuse, Tomonari Hirano, Yoichiro Hoshino, and Hisato Kunitake

significant difference from 2 to 4 h. Fig. 2. Gene expression of candidates, copper/zinc superoxide dismutase ( Cu/Zn SOD ), iron SOD ( Fe SOD ), manganese SOD ( Mn SOD ), catalase ( CAT ), cysteine protease ( CYP ), l -galactose-1-phosphate

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Ting-Ting Li, Zhi-Rong Li, Kang-Di Hu, Lan-Ying Hu, Xiao-Yan Chen, Yan-Hong Li, Ying Yang, Feng Yang, and Hua Zhang

by ethylene production and protein degradation. To study whether H 2 S has an effect on ethylene synthesis pathway and protein degradation, expressions of AdSAM , ACS genes AdACS1–3 and ACO genes AdACO1–3 , and cysteine protease genes AdCP1