photoperiod at 16/8 h light/dark with the light intensity of 100 μmol·m −2 ·s −1 . After 30 d, newly formed shoots were used for subsequent experiments. Microshoot proliferation. Established microshoots of P. coccinea were dissected under a laminar hood and
used to propagate this species. The aim of this study was to develop in vitro propagation of C. erectus through axillary shoot proliferation. Materials and Methods Plant material, surface disinfection, and culture establishment. Shoot tips, 3–5 cm
., 2003 ; Wang et al., 2005 ), inhibited proliferation of the human lung epithelial cancer cell line A549, and decreased tetradecanoylphorbol-13-acetate (TPA)-induced neoplastic transformation of JB6 P + mouse epidermal cells ( Wang et al., 2005
Station, Tabriz, Iran. Nodal segments of 0.5 to 1 cm lengths, including axillary buds, were used as explants. Establishment and proliferation of HS314 rootstock shoots in vitro. Explants were surface-sterilized by immersing them in 70% (v/v) ethanol for 1
commercial production. Tissue culture is a rapid and efficient way to propagate C. goeringii . During propagation of C. goeringii , mature seeds are often used as the material to obtain sterile rhizomes for further proliferation and differentiation. The
axillary shoot proliferation. The latter method was used because it yields a high level of genetic stability ( Lloyd and McCown, 1980 ) and, during proliferation, stimulation of areas of the axillary buds in contact with the medium are synchronized
rhizome proliferation, differentiation, and rooting. Among numerous factors, plant growth modulators such as auxins and cytokinins play key roles in vitro ( Shimasaki and Uemoto, 1990 ). Auxins such as 2,4-dichlorophenoxyacetic acid (2,4-D) and
/PLB; however, their slow growth and proliferation necessitate improvement of the culture procedure ( Teixeira da Silva, 2013a ). Therefore, several plant growth regulators PGRs) such as benzyladenine (BA), adenine sulphate (AS), chitosan (CS), thidiazuron (TDZ
shoots proliferation ( Kapoor and Rao, 2006 ; Nadgauda et al., 1990 ; Sood et al., 2002 ). However, it is very difficult to obtain bamboo reproductive tissues in the field. Lin and Chang (1998) used field-grown vegetative shoot meristems to induce
traits were measured again in addition to the aerial root numbers. Shoot proliferation was also scored based on the height of the growth in the culture tube [1 = 1–10 mm, 2 = 11–20 mm, 3 = 21–30 mm, 4 = 31–40 mm, and 5 = 41–50 mm ( Figs. 5 and 6 )]. The