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Toshiki Asao, Hiroaki Kitazawa, Kazuyori Ushio, Yukio Sueda, Takuya Ban, and M. Habibur Rahman Pramanik

cultivated with prairie gentian for over 10 years in Nagano prefecture, Japan, and was used as medium of growth for the bioassay. Three kilograms of the soil was pulverized and placed in each plastic container (17 cm × 29 cm × 9.5 cm) after amending with AC

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David B. Rubino

Segregating lisianthus [Eustoma grandiflorum (Griseb.) Shinn.] progeny were evaluated to determine the inheritance of esterase (EST), diaphorase (DIA), and glucose-6-phosphate isomerase (GPI) isozymes. Phenotypic data supported the hypotheses that EST is monomeric and controlled by one locus (Est1) with at least three alleles, DIA is tetrameric and controlled by one locus (Dia2) with at least two alleles, and GPI is controlled by one locus (Gpil) with at least two alleles. The structure of the GPI isozyme could not be inferred from banding patterns. Joint segregation analyses indicated that the three loci segregate independently. These three isozymes are the first simply inherited, unlinked biochemical markers identified in lisianthus. These marker loci will be useful for genetic studies, breeding, and germplasm characterization.

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Terri Woods Starman

One and two foliar spray and single-drench applications of uniconazole were applied to Eustoma grandiflorum (Raf.) Shinn (lisianthus) `Yodel Blue' to determine optimal concentrations for potted plant height control. A single uniconazole spray at 10.0 mg·liter-1 applied 2 weeks after pinching, two uniconazole applications at 5.0 mg·liter -1 applied 2 and 3 weeks after pinching, or a drench at 1.60 mg a.i. per pot applied 2 weeks after pinching gave equally good height control. At these concentrations, uniconazole was similar in its effect on plant height to daminozide foliar sprays at 7500 and 2500 mg·liter-l applied once and twice, respectively. Drenching with uniconazole at 1.60 mg a.i. per pot did not increase days to flower (DTF), whereas foliar spray applications did. Drenching did not reduce flower size, but increased flower number at time of harvest. Chemical names used: α-cyclopropyl-α-(4-methoxyphenyl)-5-pyrimidinemethanol (ancymidol); butanedioic acid mono(2,2-dimethylhydrazide) (daminozide);(E)-(S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-pent-1-ene-3-01 (uniconazole).

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Xiaojie Zhao, Guihong Bi, Richard L. Harkess, and Eugene K. Blythe

, 2010 ; Niu et al., 2011 ). In prairie gentian ( Eustoma grandiflorum ), the dry weight of leaf, stem, and shoot increased linearly with increasing NH 4 :NO 3 ratios ( Mendoza-Villarreal et al., 2015 ). In addition, if available N form was not the one

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Reza Saeedi, Nematollah Etemadi, Ali Nikbakht, Amir H. Khoshgoftarmanesh, and Mohammad R. Sabzalian

The genus Eustoma belongs to Gentianaceae family and includes two species, i.e., E. exaltatum L. and E. grandiflorum (Raf.) Shinn, commonly known as Eustoma, lisianthus, prairie gentian, Texas bluebell ( Dole and Wilkins, 2004 ). Lisianthus is