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Elisabeth M. Meyer, Darren H. Touchell and Thomas G. Ranney

ornamental characteristics such as larger, longer-lasting flowers, thicker petals, and larger, thicker leaves ( Kehr, 1996 ). In addition, polyploid plants, particularly allopolyploids, can have other advantageous traits such as enhanced vigor, improved pest

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Dave I. Thompson, Neil O. Anderson and Johannes Van Staden

; Sparnaaij, 1979 ). Because naturally occurring polyploid genotypes are usually unavailable for breeding programs, polyploidy is typically induced through mitotic spindle inhibition or microtubule polymerization, often by exposure to colchicine ( Caperta et

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Cary J. Hebert, Darren H. Touchell, Thomas G. Ranney and Anthony V. LeBude

) develop an efficient methodology for in vitro shoot regeneration from leaves of R . ‘Fragrantissimum Improved’; and 2) develop an oryzalin-mediated protocol for polyploid induction in ‘Fragrantissimum Improved’. Materials and Methods Plant

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David M. Czarnecki II and Zhanao Deng

( Sharma et al., 2005 ). Polyploid manipulation, particularly triploid production, has been proposed as a genetic approach to develop sterile, noninvasive lantana cultivars ( Czarnecki et al., 2008 ). Similar genetic approaches (polyploid production and

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Guo-Gui Ning, Xue-Ping Shi, Hui-Rong Hu, Yan Yan and Man-Zhu Bao

and Whitton, 2000 ). The manipulation of ploidy can also be a valuable tool for plant breeding programs. Polyploids often generate variants that may contain useful characteristics and provide a wider germplasm base for breeding studies ( Ramanna and

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Zhitong Li and John M. Ruter

blooming vigor and longevity. Induction of polyploid genotypes is an effective means of producing enhanced characteristics and increased sterility. In breeding ornamental plants, polyploid induction has been widely applied to enlarge plant organs ( Caporali

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Jonathan D. Mahoney, Thao M. Hau, Bryan A. Connolly and Mark H. Brand

( Dirr, 2009 ; McKay, 2001 ). Aronia flowers are thought to be protogynous and self-compatible ( Connolly, 2014 ). Polyploid Aronia species have been reported to reproduce apomictically, via gametophytic apomixis, resulting in embryos that are

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Jin-Hu Wu, A. Ross Ferguson, Brian G. Murray, Alison M. Duffy, Yilin Jia, Canhong Cheng and Philip J. Martin

ripening earlier ( Li et al., 2002 ). The reduction in fruit firmness and DM could be the result of the fruit of autotetraploids having larger cells with larger intercellular spaces; perceived flesh texture could likewise be affected. Polyploids generally

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David M. Czarnecki II, Amanda J. Hershberger, Carol D. Robacker, David G. Clark and Zhanao Deng

Caprice’. Most of the meiotic work was conducted at the University of Georgia (Athens and Griffin, GA). Results Ploidy analysis. A polyploid series was observed among the 26 commercial cultivars and six UF breeding lines analyzed for ploidy level ( Table 1

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Mario I. Buteler, Don R. LaBonte and James H. Oard

RAPD and the single dose polymorphic band (SDPB) are powerful tools for genome map construction of higher polyploids, such as hexaploid sweetpotato. Duplication in the genome of higher polyploids results in fewer polymorphisms per primer screened than one would expect in diploids. The Stoffel fragment (Sf) is suggested as an alternative to the most commonly used Taq DNA polymerase to maximize the number of polymorphisms. Genomic DNA from two sweetpotato varieties, `Excel' and `Beauregard', and F1 progeny was isolated using a modified CTAB procedure. The DNA was assayed with twelve primers from Operon Technologies groups A and F. Each enzyme was tested with and without a ramp temperature treatment between the annealing and the extension temperatures. Results are based on three separate amplifications and electrophoretic runs. Band reproducibility was better using Sf than Taq; unfortunately, resolution was lower making bands difficult to score. 8.4% more scorable bands and 20.3% more storable polymorphisms were obtained with Taq. The ramp treatment did not alter results using Sf, but did improve the reproducibility of Taq and ease scoring. The number of bands and their location were the same.