Search Results

You are looking at 1 - 10 of 556 items for :

  • "polymerase chain reaction (PCR)" x
  • All content x
Clear All
Free access

Hatsuhiko Okada, Yoshitaka Ohashi, Mamoru Sato, Hideyuki Matsuno, Toshiya Yamamoto, Hoytaek Kim, Tatsuro Tukuni, and Sadao Komori

were to confirm the ploidy level of the androgenic genotypes by flow cytometry; to confirm the zygosity state of the androgenic genotypes by SSR markers; to analyze S -alleles of the androgenic genotypes by the specific polymerase chain reaction (PCR

Full access

Yusuke Ii, Yuichi Uno, Michio Kanechi, and Noboru Inagaki

that endogenous ribosomal RNA fragments were observed in all samples. This result indicates that the multiplex PCR used in this study is consistent between the two cultivars tested. Fig. 1. Fragments amplified by polymerase chain reaction (PCR) using

Free access

Xiaoling He, Susan C. Miyasaka, Yi Zou, Maureen M.M. Fitch, and Yun J. Zhu

chain reaction analysis. To determine the presence of the transgene, genomic DNA was extracted from ≈10 mg of callus or leaf tissues and analyzed using polymerase chain reaction (PCR) based on the method described by He et al. (2008

Free access

Timothy W. Coolong, Ronald R. Walcott, and William M. Randle

, alternative methods have been developed to detect B. aclada in onion bulbs. These include culturing samples on semiselective media, enzyme-linked immunosorbent assay tests, and conventional polymerase chain reaction (PCR) detection ( Kritzman and Netzer

Free access

Jollanda Effendy, Don R. La Bonte, and Niranjan Baisakh

reverse transcription polymerase chain reaction (PCR) analysis of differentially expressed genes in storage root of sweetpotato at 0, 2, 4, 8, and 12 h after skinning injury. Elongation factor gene (EF) was used as the endogenous reference for cDNA loading

Free access

Douglas C. Whitaker, Mihai C. Giurcanu, Linda J. Young, Pedro Gonzalez, Ed Etxeberria, Pamela Roberts, Katherine Hendricks, and Felix Roman

samples throughout the state. Figure 1 shows the classification of samples according to their starch content and their PCR analysis during the two time periods. Fig. 1. The polymerase chain reaction (PCR)-based classification—positive or negative—and log

Free access

Wenhao Dai, Victoria Magnusson, and Chris Johnson

transformed and nontransformed chokecherry plants based on the method of Lodhi et al. (1994) . Polymerase chain reactions (PCRs) were carried out in 25 μL volume containing 200 μ m dNTPs, 1 μ m each of oligonucleotide primer, 2.5 units DNA Taq Polymerase

Free access

Wenhao Dai, Yuanjie Su, Hongxia Wang, and Ceilo Castillo

plants based on the method of Lodhi et al. (1994) . Reactions of polymerase chain reaction (PCR) were carried out in 25 μL volume containing 200 μM dNTPs, 1 μM of each oligonucleotide primer, 2.5 units Taq DNA Polymerase (Promega, Madison, WI), and 25

Free access

Júlia Halász, Andrzej Pedryc, Sezai Ercisli, Kadir Ugurtan Yilmaz, and Attila Hegedűs

growth analyses have led to uncertainty of the self-incompatibility genotypes for many Turkish cultivars ( Misirli et al., 2006 ). In this study, we used polymerase chain reaction (PCR) amplification of the S-RNase intron regions to determine their

Free access

Fatemeh Khodadadi, Masoud Tohidfar, Mehdi Mohayeji, Abhaya M. Dandekar, Charles A. Leslie, Daniel A. Kluepfel, Timothy Butterfield, and Kourosh Vahdati

. 2. Gel electrophoresis separation of polymerase chain reaction (PCR) products obtained using P14a and JrPPO primers and DNA extracted from two walnut cultivars, Serr and Chandler. PCR products sizes were 500 and 598 bp, respectively. Results showed