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David M. Czarnecki II, Amanda J. Hershberger, Carol D. Robacker, David G. Clark, and Zhanao Deng

pollen that can be transferred onto native lantana’s flowers by pollinators. Dehgan (2006) indicated the existence of a wide range of pollen stainability (from less than 5% in Patriot™ ‘Sunburst’ to more than 80% in ‘Professor Raoux’) in L . camara

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M. L. Weaver, H. Timm, M. J. Silbernagel, and D. W. Burke


Viability of pollen grains of isogenic sibling bean (Phaseolus vulgaris L.) selections of known tolerance of sensitivity to high temperatures (HT), as previously determined by pod retention and seed yield, was compared to that of a common parent bean selection and a cowpea [Vigna unguiculata (L.) Walp.] cultivar. Exposure of newly opened flowers to temperatures of 35° or 41°C reduced the viability of pollen grains in all bean selections. Pollen of all sibling selections was less affected by HT than pollen of their common parent suggesting transgressive segregation of factors for HT tolerance. At 41°, most pollen grains were destroyed in the parent bean selection and the 2 HT-sensitive siblings, whereas 44% to 55% of the pollen grains appeared to be viable in the 2 HT-tolerant siblings. Pollen viability of the HT-tolerant cowpea cultivar was not reduced by temperatures to 41°. Pollen staining indicated an interrelationship between pollen viability and tolerance to HT stress among the bean selections. The technique described has the potential for rapid selection of HT-tolerant genotypes in hybrid populations.

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Katsumi Suzuki, Tadashi Tsukaguchi, Hiroyuki Takeda, and Yoshinobu Egawa

Pod yield of `Kentucky Wonder' green bean (Phaseolus vulgaris L.) decreased at high temperatures due to a reduction of pod set. A highly positive correlation was observed between pod set and pollen stainability in flowers that were affected by heat stress about 10 days before anthesis. Pollen stainability was decreased by heat stress applied 8 to 11 days before flowering under controlled environment conditions. When mean air temperature during this period exceeded 28 °C, pollen stainability decreased under field conditions. Low pollen stainability indicated sensitivity to high temperatures about 10 days before flowering. A heat-tolerant cultivar showed higher pollen stainability than did heat-sensitive cultivars under high temperatures. These results demonstrated that heat tolerance at an early reproductive stage could be evaluated by analyzing pollen stainability using flowers developed under high temperatures.

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Carlee Steppe, Sandra B. Wilson, Zhanao Deng, Keri Druffel, and Gary W. Knox

lantana in Australia also differed dramatically in pollen viability and ploidy level/chromosome number. The weedy form had ≈65% pollen stainability and was a tetraploid with 2 n = 4 x = 48 chromosomes, whereas the garden form had extremely low pollen

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Renjuan Qian, S. Brooks Parrish, Sandra B. Wilson, Gary W. Knox, and Zhanao Deng

stainability on these porterweeds, which would be useful data in the breeding of noninvasive plants. Pollen staining has become a reliable method of determining pollen viability in hybridization studies ( Czarnecki et al., 2014 ). Other porterweed studies have

Open access

S. Brooks Parrish and Zhanao Deng

, we examined their leaf morphology, stomata size and density, pollen stainability, chromosome number and nuclear DNA content, and SSR banding pattern. These findings will have major implications on caladium breeding, their possible origins, and

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Hirotoshi Tsuda, Hisato Kunitake, Mai Yamasaki, Haruki Komatsu, and Katsunori Yoshioka

compare plant morphological characteristics and pollen stainability of these plants and their parents. Materials and Methods Plant materials. The seeds collected from wild-type shashanbo at Yame city, Fukuoka prefecture, Japan, were sterilized with sodium

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Sarah M. Smith and Zhanao Deng

their F 1 hybrids showed reduced pollen stainability. Smith (1976) indicated the existence of several structural differences, including reciprocal translocations, between the chromosomes of COLE and COTI. Thus, some levels of male and/or female

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Hsiang-I Lee and Michael J. Havey

pollen stainability in onion families possessing S cytoplasm and segregating for the dominant allele at Ms from three different sources. We observed that male fertility restoration conditioned by one source of the dominant Ms allele was incomplete and

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Boniface B. Dumpe and Rodomiro Ortiz

Current efforts to produce improved genotypes of plantain and banana (Musa spp.) depend on crossing female-fertile clones with accessions that produce viable pollen. Musa accessions (168) were screened for production of viable pollen based on staining with acetocarmine glycerol jelly. Diploid hybrids and landraces produced significantly more pollen than triploids and tetraploids, suggesting more successful crosses when using diploid accessions as male parents. There was a positive correlation between the amount of pollen produced and the level of viability in both hybrids (r = 0.65, P≤ 0.01) and landraces (r = 0.61, P≤ 0.01). This finding suggests that closely associated genetic factors determine these characteristics in Musa, while environmental conditions also may influence the quality and quantity of pollen produced. Pollen production at anthesis was absent in 28 accessions. Of the 140 accessions with pollen, 67 were sufficiently fertile for use as male parents in the breeding program.