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Yiran Li, Asuka Uchida, Akiha Abe, Akihiro Yamamoto, Tomonari Hirano and Hisato Kunitake

pollen culture system to provide a stable environment for pollen grain growth and succeeded in simulating SI-like responses in vitro using stylar crude protein extracts ( Uchida et al., 2012 ). A high inhibition rate was observed after incompatible

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Yiran Li, Akiha Abe, Takuichi Fuse, Tomonari Hirano, Yoichiro Hoshino and Hisato Kunitake

-compatible to SI 3 to 5 d before anthesis ( Uchida et al., 2012b ). Moreover, for investigation of Citrus pollen tubes, we have established an in vitro pollen culture system ( Uchida et al., 2012a ) and an in vitro SI-like reaction system using the stylar