Effects of different plant oils (soybean oil, corn oil, olive oil, peanut oil, linseed oil, and cotton seed oil) and oil component emulsions on scald development in `Delicious' apples were studied. Prestorage treatment with commercial plant oils reduced scald development, but was not as effective as 2000 mg•L-1 diphenylamine (DPA) after 6 months of cold storage. Different oil components played different roles in affecting scald. At 6% or 9% concentrations, neutral lipids (mono-, di-, and tri-acylglycerols), and phospholipids inhibited scald to the same level of 2000 mg•L-1 DPA treatment. Free fatty acids partially reduced scald, while α-tocopherol at 3% or higher concentrations accelerated scald development. There were no differences in scald inhibition between unsaturated neutral lipids and saturated neutral lipids or among the different acylated neutral lipids. When α-tocopherol was stripped from plant oils, the stripped plant oils at 6% or 9% controlled scald to the same level of 2000 mg•L-1 DPA treatment. Emulsions of 6% or 9% neutral lipids, phospholipids, or stripped plant oils did not induce greasiness on fruit skin. Fruit treated with lipids, phospholipids, or stripped plant oils looked greener and fresher compared with the control by the end of storage.
Zhiguo Ju, Yousheng Duan, and Zhiqiang Ju
Cheng-lie Zhang, Paul H. Li, and Charles C. Shin
Twenty-day-old `Bush Blue Lake 47' common bean plants grown in a growth chamber at 25 days/22C night and a 12-hour photoperiod regime were foliar sprayed with 0.5% GLK-8903 including 0.05% Tween-20. After 24 hours of treatment, plants were chilled in a cold room (4C day/night, 12 hours of light). After 3 days of chilling, leaves of untreated controls were injured, as visually characterized by leaf wilting, whereas leaves of the GLK-8903-treated plants still retained turgor. During chilling, the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) decreased. GLK-8903 treatment had no effect on SOD and POD activities; however, the CAT activity was reduced significantly after GLK-8903 treatment either at 25 or at 4C. During chilling, the content of malondialdehyde, a decomposition product of phospholipid peroxidation, increased in treated plants and untreated controls, with increased content significantly lower in the former compared with the latter. The GLK-8903 per se and total lipid extracted from GLK-8903-treated plants were able to reduce the linoleic acid oxidation in vitro. The mechanism by which GLK-8903 alleviates chilling injury in bean plants is discussed.
Shiow Y. Wang and Miklos Faust
Composition changes in galactolipids, phospholipids, and sterols in apple shoots (Malus domestica Borkh. cv. Red Delicious) from August to April were determined. The predominant fatty acids in the membrane lipids of apple shoots were palmitic acid (C16:0), linoleic acid (C18:2), and linolenic acid (C18:3). The major galactolipid components in apple shoots were monogalactosyl diglyceride (MGDG) and digalactosyl diglyceride (DGDG). The amount of MGDG and DGDG increased from autumn to spring. Galactolipids contained highly unsaturated fatty adds, mainly linoleic (18:2) and linolenic (18:3) acid. The major individual phospholipids were phosphatidylcholine (PC) and phosphatidylethaeolamine (PE). β -Sitosterol and sitosteryl ester were the predominant sterols. The phloem contained higher amounts of galactolipids, phospholipids, and sterols than did the xylem tissue. There was a significant increase in the content of galactolipids and phospholipids and onsaturation of their fatty acids during cold acclimation. A decrease in the ratio of free sterols to phospholipids also occurred in apple shoots toward cold winter months. Composition changes in galactolipids, phospholipids, and sterols that were associated with growth cessation, defoliation and cold acclimation from fall to winter, were mostly reversed following deacclimation in spring.
Shiow Y. Wang and Miklos Faust
The changes of membrane lipids in apple (Malus domestics Borkh. cv. Delicious) auxillary and terminal buds from August to April were determined. The predominant lipids were monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE). An increase in membrane polar lipids was associated with budbreak and bud growth from August to April. Linolenic acid was the predominant fatty acid in MGDG, DGDG, and PC, while linoleic acid was predominant in PE. Phosphatidylglycerol (PG) and phosphatidylinositol (PI) contained a high amount of palmitic acid. The ratio of (18:2 + 18:3) to 18:1 fatty acids in galactolipids in apple buds increased from August to April. ß-Sitosterol and sitosteryl ester were the predominant sterols in apple buds. An increase in sitosterol, a decrease in sitosteryl ester, and a decline in the ratio of free sterols to phospholipids occurred during budbreak in spring. A decrease in sitosterol was associated with bud expansion in spring.
Zienab F.R. Ahmed and Jiwan P. Palta
, 2006 ; Hong and Chung, 2006 ; Hong et al., 2007 ). There is additional evidence that phospholipids and lysophospholipids such as LPE are signaling molecules that can regulate plant growth and development ( Chapman, 1998 ; Cowan, 2006a , 2006b
Bruce D. Whitaker
Lipid composition and pigment content were determined in pericarp of `Pik Red' tomatoes (Lycopersicon esculentum Mill.) that were harvested when mature-green (MG) then ripened for 1 or 14 days at 20C, chilled for 11 or 21 days at 2C, or chilled for 21 days and transferred to 20C for 4 days (rewarmed). During ripening, chlorophyll fell below a detectable level, carotenes increased 100-fold, phospholipids (PLs) dropped ≈20%, and galactolipids (GLs) dropped ≈35%. Fatty-acid unsaturation decreased slightly. Steryl esters (SEs), more than free sterols (FSs) and steryl glycosides (SGs), increased at the expense of acylated steryl glycosides (ASGs), and in all four steryl lipids, the stigmasterol: sitosterol ratio rose dramatically, whereas the level of isofucosterol fell sharply. During chilling, chlorophyll declined ≈40% and carotenes ≈60%. PL content did not change, whereas GL fell ≈15%. Fatty-acid unsaturation increased slightly. FS, much more than SG and SE, increased at the expense of ASG. The stigmasterol: sitosterol ratio changed little in ASG, SG, and SE but declined in FS. Isofucosterol increased in FS and SE. Rewarming had little effect on the levels of chlorophyll, carotenes, or PL levels, but caused GL to fall another ≈15%. Fatty-acid unsaturation decreased slightly in GL and ASG. The distribution of total sterol in ASG, SG, FS, and SE changed dramatically, yielding proportions close to those in unchilled MG fruit. Also, 4 days after rewarming, the stigmasterol: sitosterol ratio had increased sharply, particularly in FS and SE, and there was a further rise in isofucosterol in all four steryl lipids. These results indicate that chloroplast damage occurs during chilling, but PL-rich cell membranes are not degraded, even upon rewarming. Changes in sterol composition and conjugation during chilling and after rewarming could result in membrane dysfunction.
Bruce D. Whitaker
, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PL, phospholipid; SG, steryl glycoside; TSL, total steryl lipids (FS + ASG + SG). Use of a company or product name by the U.S. Dept. of Agriculture (USDA) does not imply approval or
Timothy K. Hartz and Thomas G. Bottoms
mass of carbon mineralized by microbial respiration was calculated. At the end of 7 d, 50 g of moist soil was removed from each jar. These soil samples were subjected to phospholipid fatty acid (PLFA) analysis by gas chromatography, as described by
Shiow Y. Wang and Miklos Faust
The glycolipids, phospholipids, and sterols were determined in normal and watercore-affected apple (Malus domestica Borkh. cv. Delicious). Fruit with watercore contained higher amounts of glycolipids, phospholipids, and sterols. The ratios of unsaturated to saturated fatty acids and (18:3) to (18:1 + 18:2) were lower in watercore-affected tissue than in normal tissue. The ratio of free sterols to phospholipids was higher, whereas the ratio of phosphatidylcholine to phosphatidylethanolamine was lower in watercore-affected apple. Membrane lipids were altered in watercore-affected fruit.
Amanda J. Vance and Bernadine C. Strik
and patented by Oregon State University for use in sweet cherry ( Prunus avium ) to reduce fruit splitting (referred to as “cracking” in cherries) when applied preharvest ( Kaiser et al., 2014 ). The product is a wax-based, food-grade phospholipid