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M.G. DeWald, G.A. Moore, and W.B. Sherman

Abbreviations: F, fast band; FF, fast-migrating band; FS, fast- and slow-migrating bands; PER, peroxidase; PGM, phosphoglucomutase; PI, plant introduction; S, slow band; SOD, superoxide dismutase; SS, slow-migrating band. 1 To whom reprint requests

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Robert D. Marquard

Six phosphoglucomutase phenotypes were observed in pecan [Carya illinoensis (Wangenh.) K. Koch] progeny after controlled pollinations. At least one locus (Pgm-1) is present that controls polymorphism of phosphoglucomutase (PGM) isozymes in pecan. The inheritance appears simple with three observed alleles. However, progeny produced from two crosses resulted in significant deviation from the expected segregation ratios. Out of 65 named cultivars, 61 were of a single phenotype, and two of six possible phenotypes were not observed. Only one region of PGM activity was consistently expressed by gel electrophoresis from pecan tissue.

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A. Hashemi and A. Estilai

Leaf extracts of diploid guayule were analyzed for phosphoglucomutase (PGM, EC 2.7.5.1) and menadione reductase (MNR, EC 1.6.99.2) isozymes by starch gel electrophoresis. Banding patterns of hybrids indicated that PGM is monomeric and MNR tetrameric in structure. Two codominant alleles were identified at each of two Pgm loci, designated as Pgm-2 and Pgm-3. Two codominant alleles were observed at Mnr-2; MNR-1 was monomorphic in the Parthenium argentatum genotypes analyzed.

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A.G. Manganaris, F.H. Alston, N.F. Weeden, H.S. Aldwinckle, H.L. Gustafson, and S.K. Brown

Pgm-1, the gene responsible for variation in the most anodal isozyme of phosphoglucomutase in apple (Malus spp.), is shown to lie ≈8 centiMorgans from the gene Vf, which confers apple-scab resistance. The proximity of the marker and the ease by which allozymic forms can be resolved suggest that Pgm-1 will be useful for following the inheritance of scab resistance conferred by Vf.

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Mohammed A.M. Aly, Robert G. Fjellstrom, Gale H. McGranahan, and Dan E. Parfitt

Somatic embryos derived from walnut (Juglans regia L.) ovule tissues were evaluated to determine whether they were of zygotic or maternal origin. Molecular markers were used to permit evaluation at an early stage, before whole plant development. Somatic embryos developed from potentially apomictic `Sunland' and `Cisco' ovule tissue isolated from bagged putatively unpollinated flowers. Phosphoglucomutase (PGM) isozyme analysis showed that all of these embryos, except one from each cultivar, carry the same zymotype as the maternal tissue. However, restriction fragment length polymorphism (RPLP) analysis combined with isozyme evaluation demonstrated that the tested embryos originated from zygotic rather than maternal tissues. This study demonstrates the application of molecular marker analyses, particularly RFLPs, evaluation of somatic embryo origin.

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Luz Marina Reyes and Wanda W. Collins

Abbreviations: CAT, catalase; EST, esterase; GOT, glutamate oxaloacetate transaminase; MDH, malate dehydrogenase; MNR, menadione reductase; PGI, phosphoglucose isomerase; PGM, phosphoglucomutase; SAD, shikimate dehydrogenase. 1 Graduate Assistant. 2

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Johanne C. Cousineau and Danielle J. Donnelly

Abbreviations: IDH, isocitrate dehydrogenase; MDH, malate dehydrogenase; PGI, phosphoglucoisomerase; PGM, phosphoglucomutase; TPI, triose phosphate isomerase. Partial financial support from the Natural Sciences and Engineering Research Council of

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Jude W. Grosser, Frederick G. Gmitter Jr., Franca Sesto, Xiu Xin Deng, and J.L. Chandler

Abbreviations: PEG, polyethylene glycol; PER, peroxidase; PGI, phosphoglucose isomerase; PGM, phosphoglucomutase. Florida Agricultural Experiment Station Journal Series no. R-01587. We thank X.B. Ling, S. Huang, D.C. Chen, and N. Tusa for technical

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Cyrus Samimy and James N. Cummins

Isozymes of six enzyme systems extracted from 13 apple (Malus domestica Borkh.) rootstocks were separated electrophoretically on a horizontal starch gel. Each rootstock was clearly distinguished by its unique isozyme banding patterns. All the rootstocks were distinguishable using only two of the enzyme systems, phosphoglucomutase and 6-phosphogluconate dehydrogenase, both of which exhibited considerable isozyme polymorphism.

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Chemda Degani, Ruth El-Batsri, and Shmuel Gazit

Forty-one (Mangifera indica L.) cultivars were characterized electrophoretically using the isozyme systems aconitase, isocitrate dehydrogenase, leucine aminopeptidase, phosphoglucose isomerase, phosphoglucomutase, and triosephosphate isomerase. The outcross origin of some of the mango cultivars was supported by the isozymic banding patterns. Reported parentage of some other cultivars was not consistent with their isozymic banding patterns.