An F1 population, derived from an intraspecific cross between two Alstroemeria aurea accessions, was used to map quantitative trait loci (QTL) involved in ornamental and morphological characteristics. One QTL for leaf length was mapped on linkage group three of both parents near marker E+ACCT/M+CGCA-I165 explaining 20% and 14.8% phenotypic variation. Two putative QTL were detected on leaf width on A002-3 and A002-6. One QTL and three putative QTL, involved in the leaf length/width ratio were identified accounting for 46.7% of the phenotypic variance in total. Significant interaction was observed between two QTL, S+AC/M+ACT-I162 and S+AC/M+AGA-I465 in a two-way analysis of variance (ANOVA). For the main color of the flower one QTL and putative QTL accounted for up to 60% of phenotypic variance suggesting simple genetic control of flower color. A two-way ANOVA of these QTL suggested an epistatic interaction. A QTL was detected for color of the inner side of outer lateral tepal with 26.5% of the phenotypic variance explained. This QTL was also associated with main color of the flower just below the 95% threshold value. Two QTL were detected with the Kruskal-Wallis test for the tip color of inner lateral tepal near QTL for other flower color traits. Consequently flower color traits were significantly correlated. A QTL and a putative QTL for the flower size was mapped near marker E+ACCG/M+CGCT-I193 and E+ACCG/M+CGCG-197, respectively. One putative QTL was detected for the stripe width of the inner lateral tepal.
Tae-Ho Han, Herman J. van Eck, Marjo J. De Jeu and Evert Jacobsen
Julie Graham, Mary Woodhead, Kay Smith, Joanne Russell, Bruce Marshall, Gavin Ramsay and Geoff Squire
Scottish wild red raspberry (Rubus idaeus) plants at 12 sites were re-examined 10 years after initial studies had been carried out to examine the decline in population size and to address an earlier finding that demonstrated significant population differentiation over a small scale. Ten simple sequence repeat (SSR) loci were screened on the plants and a total of 80 alleles were detected, half of which were unique to a particular population, with all populations containing unique SSR alleles. Only 18 of the 80 alleles present in the wild were found in cultivated raspberries, highlighting the genetic diversity available for future breeding. This finding makes the decline in population number observed a concern, as this unique diversity is being eroded, primarily due to human impact. An additional 17 unique alleles were identified in the cultivars that were not present in the wild individuals studied. Gene flow into one lowland site was identified by the gain of one new allele into progeny at the site, but three alleles were also lost from parents to progeny. SSR markers were used to estimate the levels of outcrossing in wild red raspberry for the first time, and confirmed that the populations studied were outcrossing, intermating populations. The nonsignificant global F IS value indicates that red raspberry is an outbreeder (global F IS = −0.117), but significant population differentiation was observed [global F ST = 0.348 (P < 0.001)]. Diversity in this crop's wild relative and the population differentiation observed may have use in the future for breeding aimed at addressing climate change scenarios, and consideration should be given to means of conserving the diversity revealed by these studies.
Rui Sun, Hui Li, Qiong Zhang, Dongmei Chen, Fengqiu Yang, Yongbo Zhao, Yi Wang, Yuepeng Han, Xinzhong Zhang and Zhenhai Han
seedlings were suspected as outbreeders and were excluded) with heavy-browning phenotype, 10, nine, and 11 seedlings appeared as recombinants and the recombination rate of the markers Hi22f04, CH02a10, and CH02b07 was 21.7%, 19.6%, and 23.9%, respectively