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Beibei Li, Jianfu Jiang, Xiucai Fan, Ying Zhang, Haisheng Sun, Guohai Zhang, and Chonghuai Liu

) Standl.] landraces revealed by simple sequence repeat markers HortScience 51 120 126 Mihaljevic, M.Z. Simon, S. Pejic, I. Carka, F. Sevo, R. Kojic, A. Preiner, D. 2013 Molecular characterization of old local grapevine varieties from south east European

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B. Khadari, A. Oukabli, M. Ater, A. Mamouni, J.P. Roger, and F. Kjellberg

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.

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Àngel Fernández i Martí, José M. Alonso, María T. Espiau, María J. Rubio-Cabetas, and Rafel Socias i Company

fingerprinting have been developed in peach and sweet cherry ( Prunus avium L.) ( Cipriani et al., 1999 ; Clarke and Tobutt, 2003 ; Downey and Iezzoni, 2000 ; Testolin et al., 2000 ) and have been successfully used for molecular characterization and genetic

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Rohollah Karimi, Ahmad Ershadi, Kourosh Vahdati, and Keith Woeste

small number of individuals Genetics 89 583 590 Nicese, F.P. Hormaza, J.I. McGranahan, G.H. 1998 Molecular characterization and genetic relatedness among walnut ( Juglans regia L.) genotypes based on RAPD markers Euphytica 101 199 206 Potter, D. Gao, F

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Rayane Barcelos Bisi, Rafael Pio, Daniela da Hora Farias, Guilherme Locatelli, Caio Morais de Alcântara Barbosa, and Welison Andrade Pereira

incompatibility between cultivars. In addition to the physiological aspects evaluated, this strategy, which is associated with molecular characterization data of S-alleles, facilitates the understanding of the reproductive system and the choice of more compatible

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Maria Jose Gonzalo, Elisabet Claveria, Antonio J. Monforte, and Ramon Dolcet-Sanjuan

-induced parthenogenesis by pollination with γ-irradiated pollen followed by in vitro embryo rescue; second, to generate a DHL population from a selected hybrid; and third, to perform its molecular characterization. Materials and Methods Plant material and pollination with

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Rosanna Freyre and Erin Tripp

The potential for natural hybridization to occur between non-native, invasive species and closely related native species is of interest to biologists, conservationists, and land managers, particularly in regions such as the southeastern United States where numerous non-native species have become serious environmental pests. To explore this potential between the invasive plant species Ruellia simplex and the closely related, sympatric Ruellia caroliniensis, we conducted a study of reproductive crossability and hybrid viability. Results indicate that the production of interspecific hybrids is possible, but only in one direction (i.e., with R. caroliniensis as the maternal parent). Artificial hybrids were weak, slow-growing, and sterile. These data suggest that it is unlikely that R. caroliniensis × R. simplex hybrids could invade the gene pool of native R. caroliniensis. We also characterized hybrids at the molecular level by sequencing parents plus F1 progeny for the nuclear ribosomal internal transcribed spacer (ITS) + 5.8S region. All hybrid genotypes formed a strongly supported clade with the maternal parent, Ruellia caroliniensis. Within this clade, hybrid individuals were not differentiable from maternal genotypes. We then examined general plant morphology of hybrid individuals and the two parents. Unlike results from the molecular characterization, there was a strong signal of hybrid intermediacy from this morphological work. We conclude that morphology but not molecular sequence data (from nrITS) can be used to distinguish the two parents and their F1 hybrids.

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Zhong-Bin Wu, Hsin-Mei Ku, Yuh-Kun Chen, Chung-Jan Chang, and Fuh-Jyh Jan

etiology of this chlorotic spot disease of pear has not been clarified. The objective of this study was to identify and characterize the causal agent of this pear disease. We report here the isolation, serological and molecular characterizations of the

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Xiao-Fang Huang, Binh Nguyen-Quoc, and Serge Yelle

Sucrose synthase (SS) is one of the key enzymes in plant carbohydrate metabolism. In maize, this enzyme is encoded by two genes, Sh1 and Sus1. We have isolated and determined the 5'-upstream sequence of maize Sus1 gene and compared it with the corresponding sequence in Sh1 gene. Sequence analysis revealed that there was a weak homology between the two promoters and no common sequence elements were found. To understand the differential regulation of the expression of the two genes, we constructed chimeric GUS fusions using the two promoters of SS genes. By using the biolistic system, we delivered these constructs into various plant tissues, and their transient expression was studied. Our results showed that the two promoters of SS genes directed tissue-specific expression in the same way that the two genes are expressed in vivo. The effectiveness of the expression of the constructs was recorded by counting the total blue expression units (blue spots) per shot and by fluorometric assays. High levels of GUS activity were detected in the immature embryos, young coleoptiles, and heterotrophic young leaves bombarded with the Sus–GUS construct. More than 100 expression units were observed in these tissues. Compared with the transient expression of the 35S promoter in the same tissue, Sus promoter activity was twice as high. Strong Sus–GUS expression was also detected in the aleurone cells of developing kernels. In contrast, the Sh-GUS construct was expressed only in the endosperm with an activity twice as high as that of Sus–GUS and 35S–GUS in the same tissue. The results will be discussed in terms of the physiological roles of the two SS isozymes in plant tissues.

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Philip Stewart, Daniel Sargent, Thomas Davis, and Kevin Folta

The molecular mechanisms governing photoperiodic flowering has been well defined in the model systems of Arabidopsis thaliana(a facultative long-day plant) and rice (a short-day plant). Photoperiodic flowering control is of great interest to strawberry (Fragaria×ananassa) breeders and growers, and the genetics of photoperiodic flowering have been well studied, indicating that response to day-length is regulated by a small number of genetic loci. Cultivated strawberry is octoploid, so identification of these loci through forward genetic analyses is not practical. Since the componentry of the flowering response is generally conserved between monocots and dicots, we may assume that similar, if not identical, systems are functioning in strawberry as well. The goal of this work is to understand how cultivars likely containing identical photoperiod-sensing components are differentially sensitive to daylength. The expression patterns of genes relevant to the floraltransition were assessed under specific photoperiod conditions to assess similarities and/or differences to the model systems.