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Ruixiang Yan, Joshua B. Gurtler, James P. Mattheis, and Xuetong Fan

The objective of the study was to evaluate the effect of trichome (fuzz) removal on the efficacy of ultraviolet-C in inactivating Escherichia coli O157:H7 on peach fruit, and quality of peach [Prunus persica (L.) Batsch, cv. PF25] fruit as affected by fuzz removal and ultraviolet-C. Peach (cultivar PF25) fruit, with and without fuzz removal, were inoculated with a five-strain cocktail of E. coli O157:H7 and treated with ultraviolet-C at doses of 0, 221, and 442 mJ/cm2. Fuzz was rubbed off using damped cloths. Survival of E. coli populations was determined at days 1, 4, and 7 at 20 °C. To study fruit quality, noninoculated fruit with and without fuzz removal were treated with ultraviolet-C at the same doses. Results demonstrated that ultraviolet-C at 442 mJ/cm2 reduced the population of E. coli by 1.2 to 1.4 log colony-forming units (CFU)/fruit on peach with fuzz, and 0.9 to 1.1 log CFU/fruit on fruit without fuzz 1 day after ultraviolet-C treatment. However, E. coli populations of all samples were similar with additional storage time, resulting in no significant difference among the treatments after 7 days of storage at 20 °C. Ultraviolet-C at doses up to 442 mJ/cm2 did not have any significant effect on the surface color of peaches during 7 days of storage, although fruit with fuzz removal increased L*, hue, and chroma values. In addition, fuzz removal promoted the loss of firmness during storage. Furthermore, ultraviolet-C at 442 mJ/cm2 increased antioxidant capacity of peach skin with fuzz. Overall, our results suggested that fuzz removal had marginal effects on the efficacy of ultraviolet-C, and ultraviolet-C did not negatively affect the quality of peaches.

Free access

M. A. L. Smith, J. Reid, A. Hansen, Z. Li, and D. L. Madhavi

Industrial-scale cultivation of plant cells for valuable product recovery (e.g. natural pigments, pharmaceutical compounds) can only be considered commercially-feasible when a fully-automated, predictable bioprocess is achieved. Automation of cell selection, quantification, and sorting procedures, and pinpointing of optimal microenvironmental regimes can be approached via machine vision. Macroscopic staging of Ajuga reptans callus masses (ranging between 2-6 g FW) permitted simultaneous rapid capture of top and side views. Area data used in a linear regression model yielded a reliable, non-destructive estimate of fresh mass. Suspension culture images from the same cell line were microscopically imaged at 4x (with an inverted microscope). Using color machine vision, the HSI (hue-saturation-intensity) coordinates were used to successfully separate pigmented cells and aggregates from non-pigmented cells, aggregates, and background debris. Time-course sampling of a routine suspension culture consistently allowed pigmented cells to be detected, and intensity could be correlated with the degree of pigmentation as verified using spectrophotometer analysis of parallel samples.

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Kendra M. Blaker and James W. Olmstead

blue stages of development. z Fig. 1. Microscopic images of 12-μm sections from mature green fruit of standard texture southern highbush blueberry genotypes (100× magnification): ( A ) ‘Springhigh’, ( B ) ‘Windsor’, ( C ) ‘Star’, ( D ) FL06-245, ( E

Free access

Chalita Sriladda, Heidi A. Kratsch, Steven R. Larson, Thomas A. Monaco, FenAnn Shen, and Roger K. Kjelgren

fixed in formalin-aceto-alcohol solution in the field. The fixed leaf tissue was subjected to critical point drying using Samdri-PVT-3D (Tousimis, Rockville, MD). Microscopic images of leaf structure were collected from the SEM at the Nanoelectronics

Open access

Youngsuk Lee, Hun Joong Kweon, Moo-Yong Park, and Dongyong Lee

content. Fig. 6. Leaf morphological differences as shown by a microscopic image comparison among three apple cultivars: (A) Arisoo, (B) Fuji, and (C) Hongro. Leaf polarity thickness, extra palisade layer, differences in leaf thickness, cell density of

Free access

Yajun Chen, Jingjin Yu, and Bingru Huang

transpiration either under well-watered conditions or drought stress. However, CO 2 -induced stomatal closure may inhibit photosynthetic capacity under drought stress and photosynthetic recovery after rewatering, as discussed subsequently. Fig. 5. Microscopic

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Christine Yung-Ting Yen, Terri W. Starman, Yin-Tung Wang, Andreas Holzenburg, and Genhua Niu

methods and thorough observations are lacking. In this study, a histological protocol that accelerated tissue processing by microwave assistance and prepared exquisite microscopic images was developed. The protocol allowed excellent structural preservation

Open access

Tao Yuan, Qiuying Wei, and Gary Bauchan

). The exposed CO and hole that the AP-S will grow through ( D ). Bar = 0.5 mm. The seedcoat was observed using LT-SEM according to the methods described by Bolton et al. (2014) and Roh et al. (2012) . Microscopic images were obtained from two seeds

Full access

Shuresh Ghimire, Arnold M. Saxton, Annette L. Wszelaki, Jenny C. Moore, and Carol A. Miles

observed through physical strength testing, microscopic imaging, or sizable macroscopic alteration of morphology) during the growing season ( Anzalone et al., 2010 ; Cowan et al., 2014 ; Jenni et al., 2007 ; Kasirajan and Ngouajio, 2012 ; Li et al

Free access

Carol Miles, Russ Wallace, Annette Wszelaki, Jeffrey Martin, Jeremy Cowan, Tom Walters, and Debra Inglis

and biodegradation, “deterioration” is the loss of physical or mechanical strength as observed through physical testing, microscopic imaging, or visual assessment and may be the result of abiotic and/or biotic factors. The use of degradable or