Search Results

unresolved ( Li et al., 2007 ; Ren and Guan, 2008 ). The identification of molecular markers linked to important morphological traits will greatly facilitate maker-assisted breeding aimed at cultivar improvement ( Yu et al., 2000 ). Microsatellite or

., 2003 ). In this report, we describe the development of the first polymorphic microsatellite markers for ongoing molecular genetic research in kentucky bluegrass. Total genomic DNA was extracted from a single plant of the kentucky bluegrass cultivar

Jung, 2004 ) between genetic diversity assessed by RAPD markers and the PTM kentucky bluegrass classification system. In the current study we used microsatellite, or SSR, markers to study the genetic relationships of 247 kentucky bluegrass cultivars and

critical, and a set of molecular markers is also required. Microsatellites or simple sequence repeats (SSRs) are DNA segments containing short sequence repeats (1–6 nucleotides) and have been widely recognized as powerful and informative markers due to

variation in chromosome number and genome size among species of the Betulaceae, some degree of microsatellite marker transferability is expected based on results in other plant families. Microsatellites, also known as simple sequence repeats (SSR), are

reliable means for cultivar identification and to assess genetic relatedness and diversity in these collections. Microsatellite markers were only recently developed from an expressed sequence tag library of ‘Merton Thornless’ ( Lewers et al., 2008 ). A

Microsatellites, also known as single sequence repeats (SSRs), are a small array of one to six tandemly arranged bases spread throughout the genomes ( Dietrick et al., 1992 ). The development of SSR-based markers has become increasingly accessible

-based aneuploid ( Zhang et al., 2013 ), indicating a complicated genetic background. Therefore, the read and data analysis is a major problem for the SSR analysis of chrysanthemums. Fortunately, a powerful method called microsatellite DNA allele counting

. Molecular tools are increasingly used for crape myrtle cultivar identification, parentage comparison, and interspecific hybrid analysis ( Pooler, 2003 ; Pounders et al., 2007 ). We report the development of microsatellites from crape myrtle ‘Natchez’ ( L

length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and microsatellites or simple sequence repeat (SSR)]. RAPD and AFLP markers are dominant and present limited reproducibility ( Jones et al