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Kuaifei Xia, Xiulin Ye and Mingyong Zhang

Microsatellites, also known as single sequence repeats (SSRs), are a small array of one to six tandemly arranged bases spread throughout the genomes ( Dietrick et al., 1992 ). The development of SSR-based markers has become increasingly accessible

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Wei Zhou, Hong Wang, De-Zhu Li, Jun-Bo Yang and Wei Zhou

developed 13 microsatellite markers from L. pinceana and tested their use in another Luculia species, L. yunnanensis Hu, which is also a distylous species. Materials and Methods Genomic DNA samples of L. pinceana were extracted from silica gel

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Nian Wang, Zhang Chang Qin, Jun-bo Yang and Jing-li Zhang

. Simple sequence repeats (SSRs; microsatellites) are the favored type of molecular marker for identifying plant germplasm ( Dikshit et al., 2007 ). From a horticultural perspective, R. delavayi provides abundant genetic resources for breeding new

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Long-na Li, Songjun Zeng, Feng Zheng, Zhi-lin Chen, Kun-lin Wu, Jian-xia Zhang and June Duan

range of the wi1d populations. Determining the genetic diversity of the remaining wild plants is a critical step in developing effective conservation strategies for P. concolor as well as many other species. Microsatellites are ideal markers for

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Yuan Huang, Xue-qin Wang, Chun-yan Yang and Chun-lin Long

understood. Here, 11 polymorphic microsatellite loci of P. amethystina were developed as potential tools to investigate the genetic structures of these species. Genomic DNA was extracted from leaf tissues using the cetyltrimethyl ammonium bromide (CTAB

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Xue-qin Wang, Yuan Huang and Chun-lin Long

microsatellite markers for this species to investigate the genetic diversity and genetic structures among populations and provide a potential tool for studying molecular breeding in R. decorum . Genomic DNA was extracted from leaf tissues using the

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Shinichi Masuzaki, Naoyuki Araki, Naoki Yamauchi, Naoko Yamane, Tadayuki Wako, Akio Kojima and Masayoshi Shigyo

Bulb onion (Allium cepa L.) has a very large genome composed of a high proportion of repetitive DNAs. Genetic analyses of repetitive sequences may reveal microsatellites in order to increase the number of genetic markers in onion. Thirty microsatellites were previously isolated from an onion genomic library (Fischer and Bachmann, 2000). A complete set of Japanese bunching onion (A. fistulosum) – shallot (A. cepa Aggregatum group) monosomic addition lines were used to assign these microsatellites to the chromosomes of A. cepa. Simplified PCR conditions for each microsatellite were determined and 28 of the 30 primer pairs amplified DNA fragments, of which 21 microsatellite markers were assigned to chromosomes of A. cepa. Subsequent mapping of these microsatellites will enable us to establish the chromosomal distribution of these markers.

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Zhi-Hong Gao, Zhi-Jun Shen, Zhen-Hai Han, Jing-Gui Fang, Yu-Ming Zhang and Zhen Zhang

Sequencing amplification fragments produced using simple-sequence repeat (SSR) primer pairs pchgms2 and UDP96008 in `Dayezhugan' japanese apricot showed that SSRs obtained included a microsatellite locus originally identified in peach. The microsatellite sequence homogeneity between UDP96008 in japanese apricot in this study and UDP96008 in the peach in GenBank was 98%. Twenty-four japanese apricot genotypes originating in diverse geographic areas had been identified with 14 SSR primer pairs developed in different species of Prunus. In total, 129 alleles were obtained and per primer pairs detected 2.5 alleles on the average. The results from cluster analysis showed that the genetic distance between `Nanhong' and `Zhonghong' was the closest, and cultivars from China and from Japan could not be separated completely.

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Ying Wang, Ming Kang and Hongwen Huang

critical, and a set of molecular markers is also required. Microsatellites or simple sequence repeats (SSRs) are DNA segments containing short sequence repeats (1–6 nucleotides) and have been widely recognized as powerful and informative markers due to

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Kahraman Gürcan and Shawn A. Mehlenbacher

variation in chromosome number and genome size among species of the Betulaceae, some degree of microsatellite marker transferability is expected based on results in other plant families. Microsatellites, also known as simple sequence repeats (SSR), are