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L.F. Rosal, J.E.B.P. Pinto, S.K.V. Bertolucci, L.C.B. Costa and R.M. Corrêa

in vitro micropropagation. Micropropagation of wild plants, however, can be labor-intensive and time-consuming owing to the difficulties of removing microbial contamination and of avoiding extensive oxidation of the explants. To overcome such problems

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Meltem Bayraktar

period of time. In vitro propagation of S. rebaudiana has been studied by many researchers to develop an efficient and economic micropropagation protocol in semisolid medium ( Ahmed et al., 2007 ; Giridhar et al., 2010 ; Hwang, 2006 ; Lata et al

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Yung-I Lee

this study was to develop an efficient method for micropropagation of C. formosanum using explants from adult plants. The effects of timing for explant collection, BA and thidiazuron (TDZ) concentrations on shoot multiplication, and organic

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How-Chiun Wu and Chun-Chih Lin

area and shoot and root dry weight were, respectively, 5.2, 4.6, and 3.8 times higher than those cultured under a conventional micropropagation system. Moreover, plantlets produced photoautotrophically have been shown to acclimatize better in ex vitro

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Sullivan Lynch, Rachel K. Johnston, Ron O. Determann, Jennifer M. Cruse-Sanders and Gerald S. Pullman

study were to develop a reliable micropropagation protocol for threatened Georgia aster using seeds to start cultures and to investigate the feasibility of long-term seed storage through cryopreservation. Materials and Methods Plant materials

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Cameron Northcutt, Daniel Davies, Ron Gagliardo, Kylie Bucalo, Ron O. Determann, Jennifer M. Cruse-Sanders and Gerald S. Pullman

for sale, very little literature is available concerning the propagation of Sarracenia plants using micropropagation techniques. Several researchers have attempted to develop in vitro propagation methods for Sarracenia species ( Arnold, 1989

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Ying Chen, Xinlu Chen, Fei Hu, Hua Yang, Li Yue, Robert N. Trigiano and Zong-Ming (Max) Cheng

breeding ( Portillo et al., 2007 ). Therefore, the most effective means for genetic improvement is through biotechnology. Micropropagation systems and regeneration systems through either organogenesis or somatic embryogenesis have already been established

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Samir C. Debnath

In an attempt to improve the micropropagation protocol for lingonberry (Vaccinium vitis-idaea L.) developed at the Centre, two lingonberry clones were compared for in vitro shoot proliferation on two different media supplemented with varying levels of thidiazuron (TDZ). TDZ supported proliferation at low concentrations (0.1 to 1 μm) but inhibited shoot elongation. However, usable shoots were obtained within 4 weeks by transferring shoot cluster to medium containing 1 μm zeatin. Genotypes differed significantly with respect to multiplication rate with `EL1' producing the most shoots per explant. In both genotypes, shoot proliferation was greatly influenced by explant orientation. Changing the orientation of explants from vertically upright to horizontal increased axillary shoot number, but decreased shoot height and leaf number per shoot. Proliferated shoots were rooted on a 2 peat: 1 perlite (v/v) medium, and the plantlets were acclimatized and eventually established in the greenhouse.

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Andrew Riseman and Siva Chennareddy

Protocols for in vitro propagation are reported for interspecific hybrids of Exacum L., derived from Sri Lankan taxa. Four genotypes were used to evaluate the effects of MS (Murashige and Skoog) and WP (Woody Plant) media supplemented with 2-iP, BA, or KIN during establishment and multiplication phases. In addition, rhizogenesis and associated root characteristics were evaluated using MS medium supplemented with NAA or IBA. Overall, either 2-iP or BA was significantly more effective than kinetin in establishment and shoot proliferation with significant genotype × treatment interactions present. Maximum multiplication rates were achieved in the following genotype-hormone combinations: E-6, 2 mg·L-1 BA (4.5 per explant); E-23, E-32, and E-37, 2 mg·L-1 2-iP (3.5, 2.5, and 3.6 per explant, respectively). In vitro rhizogenesis was greatest in liquid MS medium supplemented with 1 mg·L-1 NAA while significantly reduced rooting was observed with IBA supplements. Our results demonstrate that micropropagation of Sri Lankan Exacum hybrids is possible and that adequate multiplication and rooting percentages can be achieved. However, the high level of genetic variation identified requires genotype-specific media modifications. Chemical names used: benzyladenine (BA); 2-isopentenyladenine (2-iP); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA); kinetin (KIN).

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Michael E. Kane and Charles Lane

159 ORAL SESSION 45 (Abstr. 687–693) Micropropagation–Floriculture/Ornamental Horticulture