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Donglin Zhang, Michael A. Dirr, and Robert A. Price

1 Department of Botany. Maine Agricultural and Forest Experiment Station Publication 2404. The marker portion of this research was conducted in the lab of Stephen Kresovich in Griffin, Georgia. We thank Stephen Kresovich, Sharon Mitchell, Doug Butts

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Minou Hemmat, Norman F. Weeden, Herb S. Aldwinckle, and Susan K. Brown

Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the Vf gene in apple (Malus sp.) that confers resistance to five races of apple scab [Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to Vf. The most tightly linked marker in the test population, S52500, was cloned and sequenced. A linkage map of the Vf region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1. An assay was developed for Vf by multiplexing the two markers closely flanking the Vf locus. This assay has a theoretical `escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.

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Maria Jose Gonzalo, Elisabet Claveria, Antonio J. Monforte, and Ramon Dolcet-Sanjuan

individuals are obtained in only one generation ( Morrison and Evans, 1988 ). Furthermore, the incorporation of other biotechnological tools to the breeding programs such as marker-assisted selection coupled with in vitro production of DH lines accelerates the

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Mariola Plazas, Santiago Vilanova, Pietro Gramazio, Adrián Rodríguez-Burruezo, Ana Fita, Francisco J. Herraiz, Rajakapasha Ranil, Ramya Fonseka, Lahiru Niran, Hemal Fonseka, Brice Kouassi, Abou Kouassi, Auguste Kouassi, and Jaime Prohens

(prickliness) and single nucleotide polypmorphism (SNP) molecular markers. For the prickliness characterization, young leaves (≈10 cm long) of hybrid plants were evaluated using the Leaf prickles descriptor of EGGNET [European Network for Eggplant Genetic

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Mary Woodhead, Ailsa Weir, Kay Smith, Susan McCallum, Katrin MacKenzie, and Julie Graham

generated in a crossing program. In red raspberry, it can take 15 years from the time of the first cross to the release of a new cultivar ( Graham and Jennings, 2009 ). Advances in molecular genetics, in particular, the development of molecular markers, have

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Paul Skroth, Jim Nienhuis, Geunhwa Jung, and Dermont Coyne

30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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V. Meglic and R.T. Chetelat

57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management

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Kahraman Gürcan and Shawn A. Mehlenbacher

variation in chromosome number and genome size among species of the Betulaceae, some degree of microsatellite marker transferability is expected based on results in other plant families. Microsatellites, also known as simple sequence repeats (SSR), are

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Kimberly J Walters, George L. Hosfield, and James D. Kelly

30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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Shanthanu Krishna Kumar, Nathan Wojtyna, Laura Dougherty, Kenong Xu, and Gregory Peck

) marker, named Q8 , was developed, which is physically located at 11.1 Mb between genes MdPP2CH (8.7 Mb) and MdSAUR37 (11.6 Mb) on chromosome 8 in the GDDH13 apple reference genome ( Daccord et al., 2017 ; Jia et al., 2018 ). Additionally, three more