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Soon O. Park, Kevin M. Crosby, Rongfeng Huang, and T. Erik Mirkov

Male sterility is an important trait of melon in F1 hybrid seed production. Molecular markers linked to a male-sterile gene would be useful in transferring male sterility into fertile melon cultivars and breeding lines. However, markers linked to the ms-3 gene for male sterility present in melon have not been reported. Our objectives were to identify randomly amplified polymorphic DNA (RAPD) markers linked to the ms-3 gene controlling male sterility using bulked segregant analysis in an F2 population from the melon cross of line ms-3 (male-sterile) × `TAM Dulce' (male-fertile), convert the most tightly linked RAPD marker to the ms-3 gene into a sequence characterized amplified region (SCAR) marker based on a specific forward and reverse 20-mer primer pair, and confirm the linkage of the RAPD and SCAR markers with the ms-3 gene in an F2 population from the cross of line ms-3 × `Mission' (male-fertile). A single recessive gene controlling male sterility was found in F2 individuals and confirmed in F3 families. Two RAPD markers that displayed an amplified DNA fragment in the male-sterile bulk were detected to be linked to the ms-3 gene in the F2 population from the cross of line ms-3 × `TAM Dulce'. RAPD marker OAM08.650 was closely linked to the ms-3 gene at 2.1 cM. SCAR marker SOAM08.644 was developed on the basis of the specific primer pair designed from the sequence of the RAPD marker OAM08.650. The linked RAPD and SCAR markers were confirmed in the F2 population from the cross of line ms-3 × `Mission' to be consistently linked to the ms-3 gene at 5.2 cM. These markers were also present in 22 heterozygous fertile F1 plants having the ms-3 gene. The RAPD and SCAR markers linked to the ms-3 gene identified, and confirmed here could be utilized for backcrossing of male sterility into elite melon cultivars and lines for use as parents for F1 hybrid seed production.

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Samuel F. Hutton, John W. Scott, and Joshua H. Freeman

. (2013) . Five rounds of marker-assisted backcrossing were used to advance Ty-1 from the RIL into Fla. 7781, a crimson line with resistance to fusarium crown rot ( Scott and Jones, 2000 ). The material that was developed is thus, in theory, nearly 97

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Samuel F. Hutton and John W. Scott

resistance. This RIL was used for building populations to fine-map Ty-1 , and its development is described by Verlaan et al. (2013) . Fig. 1. Pedigree of Fla. 7907C. A marker-assisted backcrossing approach was used to introgress Ty-1 into Fla. 7907B. The

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Eduardo Bernal and David M. Francis

Xanthomonas with multiple physiological races recognized for some species. To address the challenge of breeding for resistance, we developed four processing tomato near-isogenic lines (NILs) with improved disease resistance to bacterial spot using a marker-assisted

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Jack E. Staub and Isabelle Y. Delannay

developed by initially crossing an elite commercial European Long Greenhouse line and PI 432858 (China; long-fruited, Northern Chinese protected-culture type) followed by marker-assisted backcrossing and self-pollination ( Delannay, 2009 ; Delannay and

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Addenda

105th Annual Conference of the American Society for Horticultural Science, Orlando, FL, 21–24 July 2008: The following changes were made to the conference schedule after the Program and Abstracts issue [HortScience 43(4)] went to press.

-linked molecular markers for geno-typing, varietal protection, and expediting molecular breeding. Services include but are not limited to marker-assisted selection; DNA marker-assisted backcrossing; DNA fingerprinting and intellectual rights protection; DNA marker

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Ana Fita, Néstor Tarín, Jaime Prohens, and Adrián Rodríguez-Burruezo

development and will be mostly suitable for more advanced students as it is focused on the evaluation and prediction of the results of marker-assisted backcross programs for the introgression of quantitative trait loci. Retromelon is directed at students of

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Jack E. Staub and Isabelle Y. Delannay

germplasm, and for mapping traits, analysis of quantitative trait loci and epistatic interactions. Literature Cited Behera, T.K. Staub, J.E. Delannay, I.Y. Chen, J.F. 2011 Marker-assisted backcross selection in an interspecific Cucumis population broadens

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Allan F. Brown, Elizabeth H. Jeffery, and John A. Juvik

)-based markers could facilitate the development of new, superior broccoli inbreds and hybrid cultivars through such strategies as marker-assisted selection and marker-assisted backcrossing. This would reduce the time required to introgress favorable alleles into

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Derek W. Barchenger, Joseph I. Said, Yang Zhang, Mingzhou Song, Franchesca A. Ortega, Yeasong Ha, Byoung-Cheorl Kang, and Paul W. Bosland

.) PLoS One 8 6 e65209 Lin, S.W. Shieh, H.C. Wang, Y.W. Tan, C.W. Schafleitner, R. Yang, W.J. Kumar, S. 2015 Restorer breeding in sweet pepper: Introgressing Rf allele from hot pepper through marker–assisted backcrossing Scientia Hort. 197 170 175 Liu, J