increase the selection efficiency. Genetic linkage maps that are essential to the detection of QTL and other applications have been constructed for nearly all economically important plants. Although there are a number of genetic maps reported in B. rapa
Jian-Feng Geng, Cheng-Song Zhu, Xiao-Wei Zhang, Yan Cheng, Yuan-Ming Zhang and Xi-Lin Hou
Soon O. Park, Hye Y. Hwang and Kevin M. Crosby
bp ( Arumuganathan and Earle, 1991 ); genetic polymorphism ranges from 10% to 15% ( Staub et al., 1997 ) and genomic length is 2276 to 3250 cM ( Staub and Meglic, 1993 ). Pitrat (1991) developed a classical melon linkage map consisting of 28
Yiqun Weng, Shanna Johnson, Jack E. Staub and Sanwen Huang
availability of the whole genome sequence in cucumber provides a platform for the development of codominant markers ( Huang et al., 2009 ). A high-resolution simple sequence repeat (SSR)-based linkage map was developed using the recombinant inbred line (RIL
Yayeh Zewdie, Michael J. Havey, James P. Prince and Maria M. Jenderek*
Garlic has been propagated exclusively by asexual means since time immemorial. The recent discovery of male fertile garlic accessions allowed studies on genetics and garlic improvement. Single nucleotide polymorphism (SNP) and random amplified polymorphic DNA (RAPD) based genetic linkage map was developed for garlic using a segregating population derived from one plant of PI 540316. Progenies segregated for male fertility and other morphological characters. Distortion of segregation was observed for most of the markers. This was expected due to the segregation of recessive deleterious alleles present in the garlic genome. The map contained 23 loci distributed on five linkage groups. It covered 319 cM with the average of 18 cM between loci. Linkage with the male fertility (Mf) locus was established with SNP marker AOB155 (26.7 cM).
Bouchaib Khadari, Amal Zine El Aabidine, Cinderella Grout, Inès Ben Sadok, Agnès Doligez, Nathalie Moutier, Sylvain Santoni and Evelyne Costes
expensive procedure. The use of molecular breeding will allow saving time and increasing selection efficiency. Linkage maps of progenies segregating for important economic traits such as fruit quality and yield are required to develop marker
Ke Cao, Lirong Wang, Gengrui Zhu, Weichao Fang, Chenwen Chen and Pei Zhao
. kansuensis ‘Honggengansutao’. A genetic linkage map was constructed by using SSR, SRAP, and RGA-sequence-tagged site (STS) markers for mapping RKN resistance. This map is a valuable tool for locating the regions involved in RKN resistance and for the
Allan F. Brown, Elizabeth H. Jeffery and John A. Juvik
-stage broccoli maturity. Existing genetic linkage maps of B. oleracea have been generated using restriction fragment length polymorphic (RFLP) markers with populations developed by crossing parents from different subspecies such as broccoli × cabbage ( B
Norman Weeden and Gail Timmerman-Vaughan
A linkage map for a set of 51 F2-derived recombinant inbred lines has been constructed from the segregation data of ≈850 morphological, isozyme, RFLP, STS, RAPD, and AFLP markers. The final map consists of seven clear linkage groups with a total length of nearly 900 cM. The wide variety of loci placed on this map permits its comparison with partial maps that have been developed in other programs. For the most part, the arrangement of loci agrees with that in previous maps, and no evidence for translocation heterozygosity in this cross is apparent. Although some clustering of markers is observed, for the most part the markers are well-distributed, and few gaps greater than 5 cM are found in the coverage. The availability of this first “complete” and highly saturated map for pea should permit more efficient comparison of the partial maps that have been generated in a number of different crosses, as well as provide a firm basis for future mapping and molecular studies in this species.
A. Levi, C. E. Thomas, A. Davis, O.U.K. Reddy, Y. Xu, X. Zhang, S. King, A. Hernandez, G. Gusmini and T. Wehner
Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. The testcross map comprises 262 markers (RAPD, ISSR, AFLP, SSR and ASRP markers) and covers 1,350 cM. The map comprises 11 large linkage groups (50.7–155.2 cM), 5 medium-size linkage groups (37.5–46.2 cM), and 16 small linkage groups (4.2–31.4 cM). Most AFLP markers are clustered on two linkage regions, while all other marker types are randomly dispersed on the genome. Many of the markers in this study are skewed from the classical (Mendelian) segregation ratio of1:1 in the testcross or the 3:1 ratio in the F2 population. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and F2 population. A cDNA library was constructed using RNA isolated from watermelon flesh 1 week (rapid cell division stage), 2 weeks (cell growth and storage deposition stage, 4 weeks (maturation stage), and 5 weeks (postmaturation stage) post pollination. Over 1,020 cDNA clones were sequenced, and were analyzed using the Basic Local Alignment Search Tool (BLAST). The sequenced cDNA clones were designated as expressed sequenced tag (EST) markers and will be used in mapping analysis of watermelon genome.
Jack E. Staub, Zhanyong Sun, Sang-Min Chung and Richard L. Lower
Genetic linkage mapping has historically been the basis for genomic investigation and the analysis of quantitative trait loci (QTL) ( Doerge, 2002 ). The construction of a detailed linkage map is, in fact, the initial step for the use of genetic